不同光照条件对脱镁叶绿酸钠光敏抑菌活性的影响
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摘要
脱镁叶绿酸钠是叶绿素代谢中间产物脱镁叶绿酸a的水溶性钠盐,具有良好的光敏抑菌活性。为探索光照条件对其抑菌活性的影响,采用菌丝生长速率法,研究在不同光照度、光波长及光照时间条件下,脱镁叶绿酸钠对忽视拟盘多毛孢Pestalotiopsis neglecta的抑制作用。结果表明:20 mg/mL的脱镁叶绿酸钠在照度为2 000 lx的白光照射下对菌丝生长的抑制率为83.7%,在黑暗条件下抑制率为77.0%,表明光照对脱镁叶绿酸钠抑菌活性有显著影响(P <0.05);此外,白光的效果好于单色光;光照度及光照时间对抑菌活性也有显著影响,其中当照度为16 000 lx且光照时间为24 h时抑菌作用最强。
Sodium pheophorbide a(SPA) is a water-soluble sodium salt of pheophorbide a, an intermediate product of chlorophyll metabolism, which has good photoactivated antimicrobial activity.To investigate the effects of illumination conditions on its antifungal activity, the mycelial growth rate method was used to study the photoactivated inhibitory effect of SPA on Pestalotiopsis neglecta under different light intensities, light wavelengths and light durations. The results showed that, when treated by 20 mg/mL SPA, the inhibition rate of mycelial growth was 83.7% under white light with an illuminance of 2 000 lx, and the inhibition rate was 77.0% under dark conditions, which indicated that light had a significant effect on the antifungal activity of SPA(P < 0.05), and the effect of white light was better than that of the monochromatic light. In addition, light intensity and duration also had significant effects on the antifungal activity of SPA, and the strongest effect was observed when the light intensity was 16 000 lx and the light duration was 24 h.
Sodium pheophorbide a(SPA) is a water-soluble sodium salt of pheophorbide a, an intermediate product of chlorophyll metabolism, which has good photoactivated antimicrobial activity.To investigate the effects of illumination conditions on its antifungal activity, the mycelial growth rate method was used to study the photoactivated inhibitory effect of SPA on Pestalotiopsis neglecta under different light intensities, light wavelengths and light durations. The results showed that, when treated by 20 mg/mL SPA, the inhibition rate of mycelial growth was 83.7% under white light with an illuminance of 2 000 lx, and the inhibition rate was 77.0% under dark conditions, which indicated that light had a significant effect on the antifungal activity of SPA(P < 0.05), and the effect of white light was better than that of the monochromatic light. In addition, light intensity and duration also had significant effects on the antifungal activity of SPA, and the strongest effect was observed when the light intensity was 16 000 lx and the light duration was 24 h.
引文
[1]RYSKOVA L, BUCHTA V, SLEZAK R. Photodynamic antimicrobial therapy[J]. Cent Eur J Biol, 2010, 5(4):400-406.
[2]汪凌云,曹德榕.卟啉类光敏剂在光动力治疗中的应用研究[J].有机化学, 2012, 32(12):2248-2264.WANG L Y, CAO D R. Research advances of porphyrin photosensitizers in photodynamic therapy[J]. Chin J Org Chem, 2012,32(12):2248-2264.
[3]雷万华,王雪松.光动力抗菌光敏剂的研究进展[J].影像科学与光化学, 2013, 31(5):321-334.LEI W H, WANG X S. Research progress of photodynamic antimicrobial photosensitizers[J]. Imaging Sci Photochem, 2013,31(5):321-334.
[4]杨静,廖美德.光活化农药的研究进展[J].现代农药, 2014, 13(3):11-16.YANG J, LIAO M D. Progress in the research of photoactivated pesticides[J]. Mod Agrochem, 2014, 13(3):11-16.
[5]张喆,叶非.光活化农药的研究及进展[J].农药科学与管理, 2008,29(3):52-55.ZHANG Z, YE F. Study and development of photoactivated pesticides[J]. Pestic Sci Admin, 2008, 29(3):52-55.
[6]杨璟,张国财,胡春平,等.叶绿素降解产物二氢卟吩e6的合成及对尖孢镰刀菌的光敏抑制作用[J].吉林农业大学学报, 2018, 40(3):270-275.YANG J, ZHANG G C, HU C P, et al. Synthesis of chlorin e6 and its photosensitive antifungal activity against fusarium oxysporum[J]. J Jilin Agric Univ, 2018, 40(3):270-275.
[7]吴真,康玲,丛媛媛.蚕砂叶绿素提取及合成脱镁叶绿酸工艺研究[J].中国中医药信息杂志, 2012, 19(9):52-54.WU Z, KANG L, CONG Y Y. Improvement on extraction of chlorophyll and synthesis of pheophorbide from silkworm excrement by orthogonal experiment[J]. Chin J Inf Tradit Chin Med, 2012,19(9):52-54.
[8]圣倩倩,祝遵凌.天然脱镁叶绿酸a提取技术和测定方法的研究进展[J].应用化工, 2014, 43(7):1324-1327.SHENG Q Q, ZHU Z L. The progress of extraction technology and determination method on natural pheophorbide A[J]. Appl Chem Ind,2014, 43(7):1324-1327.
[9]姚亚红.卟啉类光敏剂的合成、表征及体外抗癌活性的研究[D].西安:西北大学, 2017.YAO Y H. Porphyrin compounds:synthesis, characterization and their anticancer activity evaluation in vitro[D]. Xi'an:Northwest University, 2017.
[10]杨君.叶绿素衍生物的合成及其初步光动力抗肿瘤作用研究[D].上海:东华大学, 2008.YANG J. Studies on synthesis and preliminary photodynamic antitumor effects of chlorophyll derivatives[D]. Shanghai:Donghua University, 2008.
[11]TANG P, ZHANG D M, XUAN N H, et al. Photodynamic therapy inhibits P-glycoprotein mediated multidrug resistance via JNK activation in human hepatocellular carcinoma using the photosensitizer pheophorbide a[J]. Mol Cancer, 2009, 8(1):56.
[12]赵占娟.新型光敏抗菌药物的研究[D].北京:北京协和医学院, 2014.ZHAO Z J. Novel photosensitizers as antimicrobial agents for photodynamic antimicrobial chemotherapy[D]. Beijing:Peking Union Medical College, 2014.
[13]WONGSINKONGMAN P, BROSSI A, WANG H K, et al. Antitumor agents. Part 209:pheophorbide-a derivatives as photo-independent cytotoxic agents[J]. Bioorg Med Chem, 2002, 10(3):583-591.
[14]赵占娟,耿文一,樊紫瑜,等.新型水溶性卟啉类光敏剂的体外抗菌作用[J].河北大学学报(自然科学版), 2017, 37(4):393-399.ZHAO Z J, GENG W Y, FAN Z Y, et al. In vitrostudy on antimicrobial activity of new water-soluble porphyrin photosensitizers[J]. J Hebei Univ(Nat Sci Ed), 2017, 37(4):393-399.
[15]李慧,李莉娜,王琪.脱镁叶绿素类化合物及其生物活性研究进展[J].时珍国医国药, 2012, 23(8):2006-2007.LI H, LI L N, WANG Q. Research progress of chlorophyll derivatives and their bioactivities[J]. Lishizhen Med Materia Medica Res, 2012,23(8):2006-2007.
[16]RATNOGLIK S L, AOKI C, SUDARMONO P, et al. Antiviral activity of extracts from Morinda citrifolia leaves and chlorophyll catabolites, pheophorbide a and pyropheophorbide a, against hepatitis C virus[J]. Microbiology&Immunology, 2014, 58(3):188-194.
[17]BOUSLAMA L, HAYASHI K, LEE J B, et al. Potent virucidal effect of pheophorbide a and pyropheophorbide a on enveloped viruses[J]. J Nat Med, 2011, 65(1):229-233.
[18]马士成.叶绿素衍生物光敏助氧化作用的生物学效应研究[D].杭州:浙江大学, 2007.MA S C. Study on biological effects of chlorophyll-a derivatives as photosensitizers and proxidants[D]. Hangzhou:Zhejiang University,2007.
[19]刘淑萍,刘岩.金属叶绿素衍生物的制备及光谱研究进展[J].河北联合大学学报(自然科学版), 2014, 36(2):84-89.LIU S P, LIU Y. Preparation and spectrum progress in the study of metal chlorophyll derivatives[J]. J Hebei United Univ(Nat Sci Ed),2014, 36(2):84-89.
[20]YAMAMOTO T, IRIUCHISHIMA T, AIZAWA S, et al. Bactericidal effect of photodynamic therapy using Na-pheophorbide a:evaluation of adequate light source[J]. Photomed Laser Surg, 2009, 27(6):849-853.
[21]王桂珍.樟子松苗木上的一种新病害:樟子松黑点针枯病[J].辽宁林业科技, 1983(Z1):41.WANG G Z. A new disease on the seedlings of Pinus sylvestris var.mongolica:black spot needle blight[J]. Liaoning For Sci Technol,1983(Z1):41.
[22]王宏.呫吨类化合物的光敏生物活性、光降解及应用研究[D].上海:上海交通大学, 2009.WANG H. Study on the phototoxicity, photodegradation and application of xanthene derivatives[D]. Shanghai:Shanghai Jiaotong University, 2009.
[23]王静,叶敏,范黎明,等.菌丝生长速率法筛选纤维素降解菌的研究[J].中国农学通报, 2013, 29(33):323-326.WANG J, YE M, FAN L M, et al. Screening of cellulose degrading fungi by mycelium growth rate method[J]. Chin Agric Sci Bull, 2013,29(33):323-326.
[24]吴铁一,屠铁城,赵红卫,等.焦脱镁叶绿酸-a作为光活化农药的光活化机理研究[J].化学学报, 2006, 64(1):17-21.WU T Y, TU T C, ZHAO H W, et al. Study on the photoactivated mechanism of a kind of photoactivated pesticide, pyrophaeophorbidea[J]. Acta Chimica sin, 2006, 64(1):17-21.
[25]丁良.两种卟啉衍生物的光谱研究[D].长春:吉林大学, 2012.DING L. The spectroscopy study of two kinds of porphyrin derivatives[D]. Changchun:Jilin University, 2012.
[2]汪凌云,曹德榕.卟啉类光敏剂在光动力治疗中的应用研究[J].有机化学, 2012, 32(12):2248-2264.WANG L Y, CAO D R. Research advances of porphyrin photosensitizers in photodynamic therapy[J]. Chin J Org Chem, 2012,32(12):2248-2264.
[3]雷万华,王雪松.光动力抗菌光敏剂的研究进展[J].影像科学与光化学, 2013, 31(5):321-334.LEI W H, WANG X S. Research progress of photodynamic antimicrobial photosensitizers[J]. Imaging Sci Photochem, 2013,31(5):321-334.
[4]杨静,廖美德.光活化农药的研究进展[J].现代农药, 2014, 13(3):11-16.YANG J, LIAO M D. Progress in the research of photoactivated pesticides[J]. Mod Agrochem, 2014, 13(3):11-16.
[5]张喆,叶非.光活化农药的研究及进展[J].农药科学与管理, 2008,29(3):52-55.ZHANG Z, YE F. Study and development of photoactivated pesticides[J]. Pestic Sci Admin, 2008, 29(3):52-55.
[6]杨璟,张国财,胡春平,等.叶绿素降解产物二氢卟吩e6的合成及对尖孢镰刀菌的光敏抑制作用[J].吉林农业大学学报, 2018, 40(3):270-275.YANG J, ZHANG G C, HU C P, et al. Synthesis of chlorin e6 and its photosensitive antifungal activity against fusarium oxysporum[J]. J Jilin Agric Univ, 2018, 40(3):270-275.
[7]吴真,康玲,丛媛媛.蚕砂叶绿素提取及合成脱镁叶绿酸工艺研究[J].中国中医药信息杂志, 2012, 19(9):52-54.WU Z, KANG L, CONG Y Y. Improvement on extraction of chlorophyll and synthesis of pheophorbide from silkworm excrement by orthogonal experiment[J]. Chin J Inf Tradit Chin Med, 2012,19(9):52-54.
[8]圣倩倩,祝遵凌.天然脱镁叶绿酸a提取技术和测定方法的研究进展[J].应用化工, 2014, 43(7):1324-1327.SHENG Q Q, ZHU Z L. The progress of extraction technology and determination method on natural pheophorbide A[J]. Appl Chem Ind,2014, 43(7):1324-1327.
[9]姚亚红.卟啉类光敏剂的合成、表征及体外抗癌活性的研究[D].西安:西北大学, 2017.YAO Y H. Porphyrin compounds:synthesis, characterization and their anticancer activity evaluation in vitro[D]. Xi'an:Northwest University, 2017.
[10]杨君.叶绿素衍生物的合成及其初步光动力抗肿瘤作用研究[D].上海:东华大学, 2008.YANG J. Studies on synthesis and preliminary photodynamic antitumor effects of chlorophyll derivatives[D]. Shanghai:Donghua University, 2008.
[11]TANG P, ZHANG D M, XUAN N H, et al. Photodynamic therapy inhibits P-glycoprotein mediated multidrug resistance via JNK activation in human hepatocellular carcinoma using the photosensitizer pheophorbide a[J]. Mol Cancer, 2009, 8(1):56.
[12]赵占娟.新型光敏抗菌药物的研究[D].北京:北京协和医学院, 2014.ZHAO Z J. Novel photosensitizers as antimicrobial agents for photodynamic antimicrobial chemotherapy[D]. Beijing:Peking Union Medical College, 2014.
[13]WONGSINKONGMAN P, BROSSI A, WANG H K, et al. Antitumor agents. Part 209:pheophorbide-a derivatives as photo-independent cytotoxic agents[J]. Bioorg Med Chem, 2002, 10(3):583-591.
[14]赵占娟,耿文一,樊紫瑜,等.新型水溶性卟啉类光敏剂的体外抗菌作用[J].河北大学学报(自然科学版), 2017, 37(4):393-399.ZHAO Z J, GENG W Y, FAN Z Y, et al. In vitrostudy on antimicrobial activity of new water-soluble porphyrin photosensitizers[J]. J Hebei Univ(Nat Sci Ed), 2017, 37(4):393-399.
[15]李慧,李莉娜,王琪.脱镁叶绿素类化合物及其生物活性研究进展[J].时珍国医国药, 2012, 23(8):2006-2007.LI H, LI L N, WANG Q. Research progress of chlorophyll derivatives and their bioactivities[J]. Lishizhen Med Materia Medica Res, 2012,23(8):2006-2007.
[16]RATNOGLIK S L, AOKI C, SUDARMONO P, et al. Antiviral activity of extracts from Morinda citrifolia leaves and chlorophyll catabolites, pheophorbide a and pyropheophorbide a, against hepatitis C virus[J]. Microbiology&Immunology, 2014, 58(3):188-194.
[17]BOUSLAMA L, HAYASHI K, LEE J B, et al. Potent virucidal effect of pheophorbide a and pyropheophorbide a on enveloped viruses[J]. J Nat Med, 2011, 65(1):229-233.
[18]马士成.叶绿素衍生物光敏助氧化作用的生物学效应研究[D].杭州:浙江大学, 2007.MA S C. Study on biological effects of chlorophyll-a derivatives as photosensitizers and proxidants[D]. Hangzhou:Zhejiang University,2007.
[19]刘淑萍,刘岩.金属叶绿素衍生物的制备及光谱研究进展[J].河北联合大学学报(自然科学版), 2014, 36(2):84-89.LIU S P, LIU Y. Preparation and spectrum progress in the study of metal chlorophyll derivatives[J]. J Hebei United Univ(Nat Sci Ed),2014, 36(2):84-89.
[20]YAMAMOTO T, IRIUCHISHIMA T, AIZAWA S, et al. Bactericidal effect of photodynamic therapy using Na-pheophorbide a:evaluation of adequate light source[J]. Photomed Laser Surg, 2009, 27(6):849-853.
[21]王桂珍.樟子松苗木上的一种新病害:樟子松黑点针枯病[J].辽宁林业科技, 1983(Z1):41.WANG G Z. A new disease on the seedlings of Pinus sylvestris var.mongolica:black spot needle blight[J]. Liaoning For Sci Technol,1983(Z1):41.
[22]王宏.呫吨类化合物的光敏生物活性、光降解及应用研究[D].上海:上海交通大学, 2009.WANG H. Study on the phototoxicity, photodegradation and application of xanthene derivatives[D]. Shanghai:Shanghai Jiaotong University, 2009.
[23]王静,叶敏,范黎明,等.菌丝生长速率法筛选纤维素降解菌的研究[J].中国农学通报, 2013, 29(33):323-326.WANG J, YE M, FAN L M, et al. Screening of cellulose degrading fungi by mycelium growth rate method[J]. Chin Agric Sci Bull, 2013,29(33):323-326.
[24]吴铁一,屠铁城,赵红卫,等.焦脱镁叶绿酸-a作为光活化农药的光活化机理研究[J].化学学报, 2006, 64(1):17-21.WU T Y, TU T C, ZHAO H W, et al. Study on the photoactivated mechanism of a kind of photoactivated pesticide, pyrophaeophorbidea[J]. Acta Chimica sin, 2006, 64(1):17-21.
[25]丁良.两种卟啉衍生物的光谱研究[D].长春:吉林大学, 2012.DING L. The spectroscopy study of two kinds of porphyrin derivatives[D]. Changchun:Jilin University, 2012.
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