基于循环水养殖系统的长鳍吻鮈亲鱼培育、催产和孵化技术初探
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摘要
2012—2015年,采用室内循环水养殖系统开展了长鳍吻鮈(Rhinogobio ventralis)亲鱼培育、人工催产和孵化技术研究。结果显示:3年间长鳍吻鮈均能在循环水养殖系统中培育成熟,2014年5月—2015年4月间培育存活率和成熟率最高,分别为85.3%~100%和77.3%~100%;对培育成熟的亲鱼进行了4种不同外源激素(PG、HCG、LRH-A2、LRH-A2+DOM)催产实验,结果为LRH-A26μg/kg+DOM 5 mg/kg合剂催产率最高,达88.3%;14、16、18、20和22℃5个温度组的受精卵孵化实验结果显示,孵化时间(H)与温度(T)呈显著负相关关系(H=0.573T2-30.393T+418.178,R2=0.982,P<0.01),18℃和20℃组的孵化率分别为54.27%和56.07%,显著高于其他各组,表明长鳍吻受精卵适宜孵化温度范围为18~20℃。
Broodstock culture,induced spawning and artificial incubation of Rhinogobio ventralis were studied in recirculating aquaculture system from 2012 to 2015. The results showed that the R. ventralis being cultured in recirculating aquaculture system could reach sexual maturity. The highest survival and maturity rate being observed during May 2014 to Apr. 2015 were 85. 3% ~ 100% and 77. 3% ~ 100%,respectively. The testing group of LRH-A26 μg/kg + DOM 5 mg/kg was the best with the highest induced spawning rate 88. 3% among four different treatments with different exogenous hormones and their combination( PG,HCG, LRH-A2,LRH-A2+ DOM) in the induced spawning experiment of R. ventralis. The effect of different water temperature( 14,16,18,20 and 22 ℃) on artificial incubation showed that there was a very significant negative correlation between hatching time( H) and water temperature( T)( H = 0. 573 T2-30. 393 T + 418. 178,R2= 0. 982,P < 0. 01). The hatching rates at 18 ℃ and 20 ℃ were 54. 27% and 56. 07%,respectively,which were significantly higher than other temperature groups. It indicated that the optimal temperature range for artificial incubation of fertilized eggs was from 18 ℃ to 20 ℃.
Broodstock culture,induced spawning and artificial incubation of Rhinogobio ventralis were studied in recirculating aquaculture system from 2012 to 2015. The results showed that the R. ventralis being cultured in recirculating aquaculture system could reach sexual maturity. The highest survival and maturity rate being observed during May 2014 to Apr. 2015 were 85. 3% ~ 100% and 77. 3% ~ 100%,respectively. The testing group of LRH-A26 μg/kg + DOM 5 mg/kg was the best with the highest induced spawning rate 88. 3% among four different treatments with different exogenous hormones and their combination( PG,HCG, LRH-A2,LRH-A2+ DOM) in the induced spawning experiment of R. ventralis. The effect of different water temperature( 14,16,18,20 and 22 ℃) on artificial incubation showed that there was a very significant negative correlation between hatching time( H) and water temperature( T)( H = 0. 573 T2-30. 393 T + 418. 178,R2= 0. 982,P < 0. 01). The hatching rates at 18 ℃ and 20 ℃ were 54. 27% and 56. 07%,respectively,which were significantly higher than other temperature groups. It indicated that the optimal temperature range for artificial incubation of fertilized eggs was from 18 ℃ to 20 ℃.
引文
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[2]曹文宣.长江上游特有鱼类自然保护区的建设及相关问题的思考[J].长江流域资源与环境,2000,9(2):131-132.
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[9]辛建峰,杨宇峰,刘焕章.长江上游长鳍吻年龄与生长的研究[J].四川动物,2010,29(3):352-356.
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[11]管敏,肖衎,胡美宏,等.长鳍吻(Rhinogobio ventralis)胚胎发育和仔鱼发育[J].渔业科学进展,2015,36(4):57-64.
[12]李菡君,邵文友,姚艳红,等.长鳍吻消化系统组织学初步研究[J].重庆师范大学学报(自然科学版),2007,24(3):13-16,页2.
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[14]洪云汉.长鳍吻的核型[J].淡水渔业,1987,17(6):17-18.
[15]Teletchea F,Fostier A,Kamler E,et al.Comparative analysis of reproductive traits in 65 freshwater fish species:application to the domestication of new fish species[J].Rev Fish Biol Fisheries,2009,19(4):403-430.
[16]刘筠.中国养殖鱼类繁殖生理学[M].北京:农业出版社,1993.
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[19]胡振禧,黄洪贵,吴妹英,等.温度对斑鳜胚胎发育的影响[J].淡水渔业,2014,44(3):104-107.
[20]谢仰杰,孙帼英.河川沙塘鳢的胚胎和胚后发育以及温度对胚胎发育的影响[J].厦门水产学院学报,1996,18(1):55-62.
[21]朱成德.不同温度对太湖短吻银鱼秋季孵化的试验研究[J].生态学报,1984,4(1):65-72.
[22]Mihelakakis A,Yoshimatsu T.Effects of salinity and temperature on incubation period,hatching rate and morphogenesis of the red sea bream[J].Aquacult Int,1998,6(2):171-177.
[2]曹文宣.长江上游特有鱼类自然保护区的建设及相关问题的思考[J].长江流域资源与环境,2000,9(2):131-132.
[3]刘军.长江上游特有鱼类受威胁及优先保护顺序的定量分析[J].中国环境科学,2004,24(4):395-399.
[4]吴强,段辛斌,徐树英,等.长江三峡库区蓄水后鱼类资源现状[J].淡水渔业,2007,37(2):70-75.
[5]鲍新国,谢文星,黄道明,等.金沙江长鳍吻的含肉率及营养成分分析[J].水生态学杂志,2011,32(5):142-148.
[6]吴兴兵,郭威,朱永久,等.长鳍吻胚胎发育特征观察[J].四川动物,2015,34(6):889-894.
[7]刘军,王剑伟,苗志国,等.长江上游宜宾江段长鳍吻种群资源量的估算[J].长江流域资源与环境,2010,19(3):276-280.
[8]辛建峰,杨宇峰,段中华,等.长江上游长鳍吻的种群特征及其物种保护[J].生态学杂志,2010,29(7):1377-1381.
[9]辛建峰,杨宇峰,刘焕章.长江上游长鳍吻年龄与生长的研究[J].四川动物,2010,29(3):352-356.
[10]徐念,史方,熊美华,等.三峡库区长鳍吻种群遗传多样性的初步研究[J].水生态学杂志,2009,2(2):113-116.
[11]管敏,肖衎,胡美宏,等.长鳍吻(Rhinogobio ventralis)胚胎发育和仔鱼发育[J].渔业科学进展,2015,36(4):57-64.
[12]李菡君,邵文友,姚艳红,等.长鳍吻消化系统组织学初步研究[J].重庆师范大学学报(自然科学版),2007,24(3):13-16,页2.
[13]邓辉胜,何学福.长江干流长鳍吻的生物学研究[J].西南农业大学学报(自然科学版),2005,27(5):704-708.
[14]洪云汉.长鳍吻的核型[J].淡水渔业,1987,17(6):17-18.
[15]Teletchea F,Fostier A,Kamler E,et al.Comparative analysis of reproductive traits in 65 freshwater fish species:application to the domestication of new fish species[J].Rev Fish Biol Fisheries,2009,19(4):403-430.
[16]刘筠.中国养殖鱼类繁殖生理学[M].北京:农业出版社,1993.
[17]欧阳海,张杨宗,谭玉均.中国池塘养鱼学[M].北京:科学出版社,1989.
[18]Bondarenko V,Drozd B,Policar T.Effect of water temperature on egg incubation time and quality of newly hatched larvae of northern pike(Esox lucius L.,1758)[J].J Appl Ichthyol,2015,31(Suppl 2):45-50.
[19]胡振禧,黄洪贵,吴妹英,等.温度对斑鳜胚胎发育的影响[J].淡水渔业,2014,44(3):104-107.
[20]谢仰杰,孙帼英.河川沙塘鳢的胚胎和胚后发育以及温度对胚胎发育的影响[J].厦门水产学院学报,1996,18(1):55-62.
[21]朱成德.不同温度对太湖短吻银鱼秋季孵化的试验研究[J].生态学报,1984,4(1):65-72.
[22]Mihelakakis A,Yoshimatsu T.Effects of salinity and temperature on incubation period,hatching rate and morphogenesis of the red sea bream[J].Aquacult Int,1998,6(2):171-177.