西番莲颗粒的急性和亚慢性毒性试验

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英文篇名：Acute and Sub-chronic Toxicity Tests of Passiflora Granules 作者：龙琰 ; 蓝毅 ; 陆明忠 ; 陈艳艳 ; 杨善忠 ; 何家康 ; 何秀苗 英文作者：LONG Yan;LAN Yi;LU Ming-zhong;CHEN Yan-yan;YANG Shan-zhong;HE Jia-kang;HE Xiu-miao;College of Marine and Biotechnology,Guangxi University For Nationalities/Key Laboratory of Microbial and Plant Resource Utilization in Guangxi Colleges and Universities;Guangxi Beidouxing Animal Health Products Limited Company;College of Animal Science and Technology,Guangxi University;Guangxi Key Laboratory Cultivation Base for Polysaccharide Materials and Modifications,Guangxi University for Nationalities; 关键词：西番莲颗粒 ; 急性毒性 ; 亚慢性毒性 ; 小鼠 ; 大鼠 英文关键词：Passiflora granule;;acute toxicity;;sub-chronic toxicity;;mouse;;rat 中文刊名：DYJZ 英文刊名：Progress in Veterinary Medicine 机构：广西民族大学海洋与生物技术学院/广西高校微生物与植物资源利用重点实验室;广西北斗星动物保健品有限公司;广西大学动物科学技术学院;广西多糖材料与改性重点实验室培育基地/广西民族大学; 出版日期：2019-01-20 出版单位：动物医学进展 年：2019 期：v.40;No.307 基金：广西自然科学基金项目(2017GXNSFAA198033);; 南宁市科学研究与技术开发计划项目(20163163) 语种：中文; 页：DYJZ201901014 页数：6 CN：01 ISSN：61-1306/S 分类号：67-72

摘要

旨在考察西潘莲颗粒对小鼠的急性毒性和大鼠的亚慢毒性作用,以评价其安全性,为临床用药提供理论依据。在急性毒性试验中,分不同浓度给小鼠灌胃给药,测试西番莲颗粒的半数致死量(LD50)和最大耐受剂量,在亚慢性毒性试验中,西番莲颗粒以3.3、1.65、0.825g/kg的剂量对SD大鼠连续灌胃4周,并观察其生理状况及其体重变化情况,在第4周周末对大鼠组织病理学、血液生化指标和血常规进行检测。结果表明,西番莲颗粒各剂量组均不引起小鼠死亡,无法测出LD50,最大耐受剂量为16.5g/kg体重;在对大鼠的亚慢毒性试验中,连续给药期间未见大鼠有不良反应,试验组大鼠在增重、血常规和血液生化指标上与对照组无显著差异或均在95%正常值范围内波动,在各脏器未发现异常组织病理学变化。说明受试药物西番莲颗粒实际无毒,安全可靠。
        This study aimed to evaluate the safety of Passifloragranules in mice and rats by acute toxicity and sub-chronic toxicity experiments,respectively,for providing theoretical basis in clinical use of Passifloragranules.In the acute toxicity study,the granules were administrated in different concentrations by intragastric method of in mice,the LD50 value and its maximum tolerance dose were evaluated.In the subchronic toxicity experiment,the granules were administrated in three concentration levels,3.3,1.65,0.825g/kg,respectively in SD rats by intragastric method for 4weeks.The physiological status and the weight gain of the rats were recorded daily or weekly during the experiment.The hematological and bio-chemical parameters were measured and the histopathological examination was performed at the end of the fourth week.As a result,there was no death or any fitness problem in mice of each group received the Passiflora granules,and the maximum tolerated dose was 16.5g/kg body weight.In the sub-chronic experiment in SD rats,there were no side effects in all the rats during the long term experiment,there were no significant differences in the body weight gains,blood routines,blood biochemical indexes between the treatment groups and control group.The histopathological result also revealed no lesions in all tissues tested.In conclusion,Passifloragranules showed no toxicity in mice and SD rats,which would be safe in clinical application.

引文

[1]陈惠芳.植物药数据库[J].国外医药:植物药分册,2005,20(6):270-273.
    [2]周玉娟,谈锋,邓君.西番莲属植物的研究进展[J].中国中药杂志,2008,33(15):1789-1793.
    [3]赵瑞瑞,邹江冰,孔秋玲,等.西番莲属植物的抗焦虑活性成分及其作用机制研究进展[J].食品与药品,2011,13(9):354-357.
    [4]蒋中仁,徐薇,金伟,等.SD大鼠血液学指标、血生化指标及脏体比正常值范围探讨[J].预防医学情报杂志,2011,27(10):789-791.
    [5]王冬平,隋丽华,尚世臣,等.清洁级SD大鼠血液生理生指标的测定[J].中国比较医学杂志,2009,19(9):44-46.
    [6]邹江冰,袁进,蒋琳兰.2种西番莲叶中黄酮的抗氧化活性研究[J].中国药房,2010,21(35):3280-3282.
    [7]邹江冰,孔秋玲,蒋琳兰.紫果西番莲叶抗焦虑药效研究[J].中华中医药学刊,2013,31(6):1332-1333.
    [8]邹江冰,袁进,蒋琳兰.紫果西番莲叶提取物抗焦虑作用及其机制[J].医药导报,2015,34(2):166-169.
    [9]许凤清,范卫卫,字成庭,等.紫果西番莲茎叶中3个新苷类化合物[J].有机化学,2018,38(2):526-530.
    [10]袁伯俊,廖明阳,李波.药物毒理学实验方法与技术[M].北京:化学工业出版社,2007.