IBDV分离株JS7的VP3基因的分子特征及其原核表达

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作者：何晨 ; 武秀知 ; 王浩 ; 何秀苗 关键词：鸡 ; 传染性法氏囊病病毒 ; VP3蛋白 ; 分子特征 ; 原核表达 中文刊名：JSNY 英文刊名：Jiangsu Agricultural Sciences 机构：广西民族大学海洋与生物技术学院/广西高校微生物与植物资源利用重点实验室;广西民族大学/广西多糖材料与改性重点实验室培育基地; 出版日期：2018-03-08 16:02 出版单位：江苏农业科学 年：2018 期：v.46 基金：国家自然科学基金(编号:31560706,31660717);; 国家大学生创新训练项目(编号:201510608031);; 广西自然科学基金(编号:2017GXNSFAA198033);; 广西民族大学相思湖青年学者创新团队项目 语种：中文; 页：JSNY201803010 页数：4 CN：03 ISSN：32-1214/S 分类号：53-56

摘要

应用RT-PCR技术扩增了鸡传染性法氏囊病病毒(IBDV)分离株JS7的VP3基因,并进行了分子特征分析和原核表达。结果显示,JS7的VP3基因的关键氨基酸位点上表现为981P、990A、1005A,既有致弱毒株的990A,又有vvIBDV毒株的981P和1005A,在遗传进化和同源性分析上,JS7更接近于vvIBDV参考株;VP3基因在IPTG的诱导下,在p ET-28a系统中成功表达出32 ku的产物,western-blot检测发现,该表达产物与鸡抗IBDV多抗血清发生反应。说明JS7-VP3成功表达并具有良好的免疫原性。这为进一步研究IBDV特异性的检测方法的建立以及VP3在IBDV致病性中的进一步研究奠定了基础。
        

引文

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