酶法辅助提取鱼腥草挥发性成分的研究及GC-MS分析
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摘要
目的:研究鱼腥草干品和鲜品中挥发性成分以及加纤维素酶辅助提取后对其挥发性成分的影响。方法:采用水蒸气蒸馏法提取鱼腥草中挥发性成分,以文献报道的最佳提取条件进行提取,GC-MS测定挥发性成分。结果:鱼腥草干品中共测出24种挥发性成分,主要成分为甲基正壬酮;鲜品中共测出30种挥发性成分,主要成分为甲基正壬酮和β-月桂烯。加纤维素酶后,干品和鲜品中挥发性成分相对百分含量都有增加。结论:鱼腥草干品和鲜品以及加纤维素酶辅助提取后主要挥发性成分基本相同,只是相对含量上存在一定的差异。
Objective: To study the volatile components of dried Houttuynia cordata dried and fresh Houttuynia cordata and the effects on volatile components by enzymatic assisted extraction. Methods: The volatile components in Houttuynia cordata were extracted by steam distillation using the optimal extraction conditions reported in the literature and then analyzing by Gas chromatography-mass spectrometry( GC-MS). Results: There were 24 kinds of volatile components in dried Houttuynia cordata detected. The main component was Methyl nononone. There were 30 kinds of volatile components in fresh Houttuynia cordata detected. The main components were Methyl nononone and β-Myrcene. After adding cellulase,the relative percentage of volatile components ih dried Houttuynia cordata and fresh Houttuynia cordata increased. Conclusions: The main volatile components of dried Houttuynia cordata and fresh Houttuynia cordata were basically the same as volatile components of Houttuynia cordata by enzymatic assisted extraction,but the percentage content was different.
Objective: To study the volatile components of dried Houttuynia cordata dried and fresh Houttuynia cordata and the effects on volatile components by enzymatic assisted extraction. Methods: The volatile components in Houttuynia cordata were extracted by steam distillation using the optimal extraction conditions reported in the literature and then analyzing by Gas chromatography-mass spectrometry( GC-MS). Results: There were 24 kinds of volatile components in dried Houttuynia cordata detected. The main component was Methyl nononone. There were 30 kinds of volatile components in fresh Houttuynia cordata detected. The main components were Methyl nononone and β-Myrcene. After adding cellulase,the relative percentage of volatile components ih dried Houttuynia cordata and fresh Houttuynia cordata increased. Conclusions: The main volatile components of dried Houttuynia cordata and fresh Houttuynia cordata were basically the same as volatile components of Houttuynia cordata by enzymatic assisted extraction,but the percentage content was different.
引文
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[2]郑亚娟,彭秋实,马义虔,等.鱼腥草化学成分的研究进展[J].广东化工,2017,44(17):85-86.
[3]王白强.酶法工艺提取鱼腥草有效杀菌成份的研究及其应用[J].福建轻纺,2008(1):20-25.
[4]何刚,卿光明,李敏,等.鱼腥草药材挥发油提取工艺优化及其成分分析[J].中药与临床,2014,5(2):20-23.
[5]孟利娜,于敬,许静,等.酶法辅助提取北苍术挥发油的工艺及成分分析[J].中成药,2013,35(4):844-847.
[6]文震,刘波,郑宗坤,等.不同方法制备的鱼腥草挥发油化学成分的研究[J].中华中医药杂志,2009,24(10):1280-1283.
[7]韦平原. GC-MS测定鱼腥草挥发油中的化学成分[J].广西医学,2012,34(7):935-936.
[8]吴艳霞.鱼腥草挥发油化学成分分析[J].黑龙江农业科学,2014(4):105-106.
[9]吕都,刘嘉,刘辉,等.鱼腥草挥发油成分分析及其抗氧化性研究[J].保鲜与加工,2016,16(6):120-124.
[10]吕雪,杨敏,刘娟.干鲜鱼腥草中挥发油抑菌作用的比较研究[J].抗感染药学,2011,8(3):180-182.
[11]薛青松.鱼腥草挥发油的研究进展[J].中国药业,2014,23(4):15-18.
[12]洪佳璇,徐淑媛,江丽,等.鲜品鱼腥草挥发油成分分析[J].浙江中医杂志,2013,48(5):375-377.