复方中草药及其与抗生素联用对斜带石斑鱼溶藻弧菌病的治疗效果
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摘要
通过药物体外抑菌效果及对斜带石斑鱼抗病力影响的研究,筛选有效治疗斜带石斑鱼溶藻弧菌病复方中草药与中西药联用配方。用诃子、白芍、甘草比例分别为0.9∶1.3∶0.9、1.2∶0.9∶1.0和1∶1∶1的复方中草药对溶藻弧菌进行体外抑菌实验;将质量分数为2.2%的复方中草药与中西药联用添加到基础饲料中设为实验组,研究中西药对斜带石斑鱼溶藻弧菌病治疗效果。复方中草药实验组Ⅰ、Ⅱ、Ⅲ为投喂饲料中添加不同比例诃子、白芍、甘草,中西药联用实验组Ⅳ、Ⅴ、Ⅵ分别为投喂饲料中添加不同比例诃子、白芍、甘草、恩诺沙星。结果显示,复方中草药配方二药物质量浓度200 mg/mL,为最佳配方。40 d病鱼死亡数量比较结果为对照组Ⅱ>对照组Ⅳ>对照组Ⅲ>实验组Ⅲ>实验组Ⅱ>实验组Ⅰ>实验组Ⅴ>实验组Ⅵ>实验组Ⅳ>对照组Ⅰ。实验组Ⅰ投喂饲料加诃子、白芍、甘草比例为0.9∶1.3∶0.9的复方中草药,实验组Ⅳ配方为诃子、白芍、甘草、恩诺沙星比例为0.9∶1.3∶0.9∶1.3,分别为治疗斜带石斑鱼溶藻弧菌病效果最好的复方中草药和中西药联用配方。研究表明,中西药联用复方药效强于复方中草药,均能较好治疗斜带石斑鱼溶藻弧菌病。
The antibacterial effect of the drugs against Vibrio alginolyticus in vitro and the drugs on the resistance to diseases of Epinephelus coioides were studied. This study aimed to screen traditional Chinese medicines formulas and Chinese and western medicines formulas which can prevent V. alginolyticus diseases of E. coioides.We had the bacteriostatic test on traditional Chinese medicines formulas against V. alginolyticus in vitro. The ratios of Terminalia chebula, Radix paeoniae and Glycyrrhiza uralensis of Chinese medicines formulas Ⅰ, Ⅱ, Ⅲwere 0.9∶1.3∶0.9, 1.2∶0.9∶1.0, and 1∶1∶1, respectively. The groups with 3 replicates per treatment of feed containing the drugs feed additives at 2.2% respectively, were fed to E. coioides. The ratio of T. chebula, R.paeoniae and G. uralensis were different in Chinese medicines formulas trial groupⅠ, Ⅱ, Ⅲ. The ratios of T.chebula, R. paeoniae, G. uralensis and enrofloxaci were different in Chinese and western medicines formulas trial groupⅣ, Ⅴ. Results revealed that the concentration of Chinese medicines formulas Ⅲ(200 mg/mL) was the best formula. The mortality for 40 d in test groups were as follows: control groupⅡ>control group Ⅳ>control groupⅢ>trial group Ⅲ>trial groupⅡ>trial groupⅠ>trial group Ⅴ>trial group Ⅵ>trial group Ⅳ>control groupⅠ. The ratio of T. chebula, R. paeoniae and G. uralensis of Chinese medicines formulaⅠwas 0.9∶1.3∶0.9. The ratios of T. chebula, R. paeoniae, G. uralensis and enrofloxaci in Chinese and western medicines formula were different.These are the best formulas. The Chinese and western medicines formula is better than the Chinese medicines formula. Results showed that by adding the formulas, the capacity of disease resistance can be improved.
The antibacterial effect of the drugs against Vibrio alginolyticus in vitro and the drugs on the resistance to diseases of Epinephelus coioides were studied. This study aimed to screen traditional Chinese medicines formulas and Chinese and western medicines formulas which can prevent V. alginolyticus diseases of E. coioides.We had the bacteriostatic test on traditional Chinese medicines formulas against V. alginolyticus in vitro. The ratios of Terminalia chebula, Radix paeoniae and Glycyrrhiza uralensis of Chinese medicines formulas Ⅰ, Ⅱ, Ⅲwere 0.9∶1.3∶0.9, 1.2∶0.9∶1.0, and 1∶1∶1, respectively. The groups with 3 replicates per treatment of feed containing the drugs feed additives at 2.2% respectively, were fed to E. coioides. The ratio of T. chebula, R.paeoniae and G. uralensis were different in Chinese medicines formulas trial groupⅠ, Ⅱ, Ⅲ. The ratios of T.chebula, R. paeoniae, G. uralensis and enrofloxaci were different in Chinese and western medicines formulas trial groupⅣ, Ⅴ. Results revealed that the concentration of Chinese medicines formulas Ⅲ(200 mg/mL) was the best formula. The mortality for 40 d in test groups were as follows: control groupⅡ>control group Ⅳ>control groupⅢ>trial group Ⅲ>trial groupⅡ>trial groupⅠ>trial group Ⅴ>trial group Ⅵ>trial group Ⅳ>control groupⅠ. The ratio of T. chebula, R. paeoniae and G. uralensis of Chinese medicines formulaⅠwas 0.9∶1.3∶0.9. The ratios of T. chebula, R. paeoniae, G. uralensis and enrofloxaci in Chinese and western medicines formula were different.These are the best formulas. The Chinese and western medicines formula is better than the Chinese medicines formula. Results showed that by adding the formulas, the capacity of disease resistance can be improved.
引文
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[6]徐先栋,谢珍玉,王世锋,等.致病溶藻弧菌脂多糖对点带石斑鱼毒性和免疫原性的影响[J].海洋科学,2010, 34(3):47-51.Xu X D, Xie Z Y, Wang S F, et al. Toxicity and immunogenicity of LPS from pathogenic Vibrio alginolyticus to grouper, Epinephelus malabaricus[J]. Marine Sciences, 2010, 34(3):47-51(in Chinese).
[7]Su B C, Lin W C, Chen J Y. Recombinant Epinephelus lanceolatus serum amyloid a as a feed additive:effects on immune gene expression and resistance to Vibrio alginolyticus infection in Epinephelus lanceolatus[J]. Fish&Shellfish Immunology, 2018, 76:233-239.
[8]Pang H Y, Qiu M S, Zhao J M, et al. Construction of a Vibrio alginolyticus hopPmaJ(hop)mutant and evaluation of its potential as a live attenuated vaccine in orangespotted grouper(Epinephelus coioides)[J]. Fish&Shellfish Immunology, 2018, 76:93-100.
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[11]周维,汤菊芬,甘桢,等.溶藻弧菌耐药基因qnr的克隆及生物信息学分析[J].生物技术,2015, 25(5):414-419.Zhou W, Tang J F, Gan Z, et al. Cloning and bioinformatics analysis of quinolone resistance gene from Vibrio alginolyticus[J]. Biotechnology, 2015, 25(5):414-419(in Chinese).
[12]李睿,翟华强,田伟兰,等.中药煮散的历史源流及其与现代配方颗粒的对比性分析[J].中国中药杂志,2016,41(5):965-969.Li R, Zhai H Q, Tian W L, et al. Comparative analysis between origin of cooked traditional Chinese medicine powder and modern formula granules[J]. China Journal of Chinese Materia Medica,2016, 41(5):965-969(in Chinese).
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