一株赛鸽新城疫病毒的分离鉴定及致病性研究

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英文篇名：Isolation,identification and pathogenicity of a Newcastle disease virus isolate from racing pigeon 作者：李珮瑶 ; 高晶萍 ; 赵丽丽 ; 田勇 ; 徐明举 ; 王存连 ; 徐彤 英文作者：LI Peiyao;GAO Jingping;ZHAO Lili;TIAN Yong;XU Mingju;WANG Cunlian;XU Tong;Key Laboratory of Preventive Veterinary Medicine,Hebei North University;Life Science Research Center,Hebei North University; 关键词：赛鸽 ; 新城疫 ; 病毒分离鉴定 ; 细菌分离鉴定 ; 人工感染 ; 毒力测定 英文关键词：racing pigeon;;Newcastle disease;;isolation and identification of virus;;isolation and identification of bacteria;;artificial infection;;virulence determination 中文刊名：HLJX 英文刊名：Heilongjiang Animal Science and Veterinary Medicine 机构：河北北方学院预防兽医学重点实验室;河北北方学院生命科学研究中心; 出版日期：2019-07-20 出版单位：黑龙江畜牧兽医 年：2019 期：No.578 基金：国家自然科学基金项目(31672522);国家自然科学基金青年科学基金项目(31602030);; 河北省第二期现代农业产业技术体系蛋肉鸡创新团队建设专项(HBCT20180207);; 河北省教育厅在读研究生创新能力资助项目(CXZZSS2018129);; 赛鸽主要疫病防控技术体系研究项目(自选课题) 语种：中文; 页：HLJX201914043 页数：6 CN：14 ISSN：23-1205/S 分类号：157-160+184-185

摘要

为了从疑似赛鸽新城疫的病料中进行新城疫病毒的分离鉴定及致病性研究,采用常规方法将24只未经任何免疫的30～40日龄雏鸽随机分成人工感染组和对照组,感染组肌肉注射分离的病毒,对照组通过相同途径接种等量生理盐水,观察临床症状,统计死亡率;对死亡病例进行剖检,取脏器组织常规处理、石蜡包埋、切片观察,测定分离毒株的鸡胚平均死亡时间(MDT)、1日龄雏鸡脑内致病指数(ICPI)、6周龄雏鸡静脉致病指数(IVPI),确定其毒力。结果表明:在疑似新城疫死亡的赛鸽体内分离出新城疫病毒,人工感染该病毒的雏鸽6 d内全部死亡,该病毒的MDT为52. 8 h,ICPI为1. 775,IVPI为2. 59。说明新城疫病毒是导致该群赛鸽死亡的主要原因,且该毒株为强毒株。
        In order to isolate and identify Newcastle disease virus and to study its pathogenicity from newcastle disease-suspected pigeon materials,conventional methods were used in this experiment for virus isolation and identification from the tissues of dead newcastle disease-suspected pigeons and for virus pathogenicity test. Twenty-four 30 to 40 day-old pigeons without any immunization were randomly divided into artificial infection group and control group. Artificial infection group pigeons received intramuscular injection of the isolated virus,and control group pigeons was inoculated with normal saline in the same way. The clinical symptoms were observed and the mortality was calculated;the autopsy,routine treatment on organs or tissues,paraffin embedding and section observation were performed on the dead cases. The mean embryo death time(MDT),intracerebral pathogenicity index(ICPI) of 1-day-old chickens,and intravenous pathogenicity index(IVPI) of 6-week-old chickens for the isolated virus determination,were used to confirm the virulence. The results showd that a Newcastle disease virus isolate was lsolated from dead newcastle disease-suspected pigeons. Artificial infection of the isolated virus led to the death of all squabs within 6 days post infection. The virus had the MDT of 52. 8 h,the ICPI of 1. 775,the IVPI of 2. 59. The results suggested that Newcastle disease virus was the main cause of the death of these pigeons,and it was a virulent strain.

引文

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