注射甘露糖结合凝集素治疗人工感染绵羊肺炎支原体的效果
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摘要
研究探讨甘露糖结合凝集素(MBL)治疗人工感染绵羊肺炎支原体的效果,揭示MBL蛋白对绵羊支原体肺炎的免疫调节作用,为阐明绵羊肺炎支原体的致病机制提供依据。人工感染绵羊肺炎支原体2周后注射MBL蛋白,对绵羊进行剖检,以及荧光定量PCR检测血浆MBL蛋白和重组MBL蛋白治疗前后,MBL、IFN-γ、TNF-α、IL-2、IL-4、C1、C3的表达变化。结果显示,注射重组MBL蛋白3周后可以减缓患病羊的病程,肺部出血斑和出血点都有轻微的减轻,与无注射治疗的对照组比较,临床症状和病理变化较轻。蛋白治疗7d后血清中IFN-γ、TNF-α、IL-2、IL-4、C3的表达水平升高,14d后呈现下降趋势,但高于对照组。MBL蛋白治疗后,患病绵羊血清中MBL、IL-2、IL-4、IFN-γ、TNF-α、C1和C3表达量均升高,随后减少,证明MBL蛋白能短期内减轻绵羊支原体肺炎症状以及增强机体的细胞和体液免疫,提高机体的抵抗力并改善炎症。
This research was to investigate the immunomodulatory effects of mannose binding lectin(MBL) on sheep experimentally infected with Mycoplasma ovipneumoniae in the hope of providing support for understanding the pathogenesis of Mycoplasma ovipneumoniae.Before injection of MBL protein,the experimental sheep were artificially infected with Mycoplasma ovipneumoniae,and autopsy was performed on the diseased sheep.Then,qRT-PCR was used to detect changes in IFN-γ,TNF-α,IL-2,IL-4,C1,C3 before and after treatment of Mycoplasma pneumoniae in sheep with plasma MBL protein and recombinant MBL protein.The results showed that three weeks after injection of recombinant MBL protein,the course of the disease in the sheep slowed down,and the pulmonary hemorrhage spots and bleeding spots in the lungs of the experimental sheep were mildly alleviated.Compared with the control group without injection,the clinical symptoms and pathological changes in the sheep were slighter.The expressions of IFN-γ,TNF-α,IL-2,IL-4 and C 3 in their serum increased seven days after protein therapy,but showed a downward trend after the 14 thday of treatment,although the expressions were higher than that of the control group.After treatment with MBL protein,the expression of MBL,IL-2,IL-4,IFN-γ,TNF-α,C 1 and C 3 in the serum of the diseased sheep increased,but then decreased,which implied that the MBL protein alleviated the symptoms of Mycoplasma pneumoniae in sheep in a short time,boosted the cellular and humoral immunity of the animals,enhanced their resistance,and lessened inflammation in their body.
This research was to investigate the immunomodulatory effects of mannose binding lectin(MBL) on sheep experimentally infected with Mycoplasma ovipneumoniae in the hope of providing support for understanding the pathogenesis of Mycoplasma ovipneumoniae.Before injection of MBL protein,the experimental sheep were artificially infected with Mycoplasma ovipneumoniae,and autopsy was performed on the diseased sheep.Then,qRT-PCR was used to detect changes in IFN-γ,TNF-α,IL-2,IL-4,C1,C3 before and after treatment of Mycoplasma pneumoniae in sheep with plasma MBL protein and recombinant MBL protein.The results showed that three weeks after injection of recombinant MBL protein,the course of the disease in the sheep slowed down,and the pulmonary hemorrhage spots and bleeding spots in the lungs of the experimental sheep were mildly alleviated.Compared with the control group without injection,the clinical symptoms and pathological changes in the sheep were slighter.The expressions of IFN-γ,TNF-α,IL-2,IL-4 and C 3 in their serum increased seven days after protein therapy,but showed a downward trend after the 14 thday of treatment,although the expressions were higher than that of the control group.After treatment with MBL protein,the expression of MBL,IL-2,IL-4,IFN-γ,TNF-α,C 1 and C 3 in the serum of the diseased sheep increased,but then decreased,which implied that the MBL protein alleviated the symptoms of Mycoplasma pneumoniae in sheep in a short time,boosted the cellular and humoral immunity of the animals,enhanced their resistance,and lessened inflammation in their body.
引文
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[3]Li Y N,Jiang Z J,Xue D,et al.Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling mediated mitochondria pathway[J].BioMed Central Microbiology,2016,16(1):222.
[4]张轩.绵羊肺炎支原体多表位疫苗的研究及免疫试验[D].北京:中国农业科学院,2013.
[5]许健,储岳峰,赵萍,等.中国部分地区绵羊肺炎支原体的基因多态性分析[J].畜牧兽医学报,2011,42(9):1295-1301.
[6]Holmskov U,Malhotra R,Sim RB,et al.Collectins:collagenous C-type lectins of the innate immune defense system[J].Immunol Today,1994,15(2):67-74.
[7]赵凤.绵羊甘露糖结合凝集素基因(MBL)与支原体肺炎的相关性研究[D].石河子:石河子大学,2011.
[8]张慧.肺炎支原体感染不同MBL基因型绵羊引起免疫因子变化规律的研究[D].石河子:石河子大学,2012.
[9]Hamvas RM,Johnson MV,lieger AM,et al.Role for mannose binding lectin in the prevention of mycolasma infection[J].Infect Immun,2005,73(8):5238-5240.
[10]陆晨.甘露糖结合蛋白研究进展[J].医学综述,2008,14(6):928-930.
[11]古丽汗·阿不都热木.MBL抗绵羊肺炎支原体感染过程中相关免疫因子的变化[D].石河子:石河子大学,2015.
[12]金遵.绵羊MBL基因真核表达载体构建及抗病相关miRNA的筛选[D].石河子:石河子大学,2016.
[13]孙恭慧.反复呼吸道感染患儿甘露糖结合凝集素与T细胞亚群的研究[J].临床荟萃,2011,26(17):1532-1534.
[14]徐燕辉.绵羊MBL基因多态性与支原体肺炎相关性研究[D].石河子:石河子大学,2007.
[15]袁焰平,任鈺为,江一波,等.猪MBL-C及DUSP1基因变异对猪繁殖与呼吸综合征病毒抗性影响的初步分析[J].动物医学进展,2013,34(4):33-38.
[16]李宇,吴芳,章乐,等.MBL基因多态性与新疆汉族人群结核病易感性的研究[J].中国人兽共患病学报,2011,27(9):769-773.
[2]Chen C,Qiao J,Meng Q L,et al.Serological and molecular survey of sheep infected with Mycoplasma ovipneumoniae in Xinjiang,China[J].Trop Anim Health Prod,2015,47(8):1641-1647.
[3]Li Y N,Jiang Z J,Xue D,et al.Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling mediated mitochondria pathway[J].BioMed Central Microbiology,2016,16(1):222.
[4]张轩.绵羊肺炎支原体多表位疫苗的研究及免疫试验[D].北京:中国农业科学院,2013.
[5]许健,储岳峰,赵萍,等.中国部分地区绵羊肺炎支原体的基因多态性分析[J].畜牧兽医学报,2011,42(9):1295-1301.
[6]Holmskov U,Malhotra R,Sim RB,et al.Collectins:collagenous C-type lectins of the innate immune defense system[J].Immunol Today,1994,15(2):67-74.
[7]赵凤.绵羊甘露糖结合凝集素基因(MBL)与支原体肺炎的相关性研究[D].石河子:石河子大学,2011.
[8]张慧.肺炎支原体感染不同MBL基因型绵羊引起免疫因子变化规律的研究[D].石河子:石河子大学,2012.
[9]Hamvas RM,Johnson MV,lieger AM,et al.Role for mannose binding lectin in the prevention of mycolasma infection[J].Infect Immun,2005,73(8):5238-5240.
[10]陆晨.甘露糖结合蛋白研究进展[J].医学综述,2008,14(6):928-930.
[11]古丽汗·阿不都热木.MBL抗绵羊肺炎支原体感染过程中相关免疫因子的变化[D].石河子:石河子大学,2015.
[12]金遵.绵羊MBL基因真核表达载体构建及抗病相关miRNA的筛选[D].石河子:石河子大学,2016.
[13]孙恭慧.反复呼吸道感染患儿甘露糖结合凝集素与T细胞亚群的研究[J].临床荟萃,2011,26(17):1532-1534.
[14]徐燕辉.绵羊MBL基因多态性与支原体肺炎相关性研究[D].石河子:石河子大学,2007.
[15]袁焰平,任鈺为,江一波,等.猪MBL-C及DUSP1基因变异对猪繁殖与呼吸综合征病毒抗性影响的初步分析[J].动物医学进展,2013,34(4):33-38.
[16]李宇,吴芳,章乐,等.MBL基因多态性与新疆汉族人群结核病易感性的研究[J].中国人兽共患病学报,2011,27(9):769-773.
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