生物转化法制备稀有人参皂苷Rh_2
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摘要
人参是最著名的中草药之一,其活性成分中含有具有抗肿瘤活性的人参皂苷Rh_2,但天然人参中Rh_2的含量不到十万分之一,十分稀少。本文以人参皂苷Rg_3为底物,加入来源于Terrabacter ginsenosidimutans的葡萄糖苷酶进行催化反应生成人参皂苷Rh_2,并对催化反应体系进行优化研究,确定了最佳反应条件:催化反应的温度40℃,pH值为7.0-8.0。本方法生产人参皂苷Rh_2工艺简单,反应选择性高,副产物少,生产成本和产品质量优于化学法,适合工业化生产。
Ginseng is one ofthe most famous Chinese herbal medicine.Its active ingredients contain ginsenoside Rh_(2 )with anti-tumor activity,but the content of Rh_2 in natural ginseng is less than 1/100000,which is very rare.In this study,ginsenoside Rg_3 was hydrolyzed to produce ginsenoside Rh_(2 )by glucosidase from Terrabacter ginsenosidimutans.The best conditions of reaction are at 40 ℃ and a pH value of 7.0-8.0.The process is simpler,higher efficient,with less by-product,lower cost than chemical catalysis.It is more applicable for industrial production.
Ginseng is one ofthe most famous Chinese herbal medicine.Its active ingredients contain ginsenoside Rh_(2 )with anti-tumor activity,but the content of Rh_2 in natural ginseng is less than 1/100000,which is very rare.In this study,ginsenoside Rg_3 was hydrolyzed to produce ginsenoside Rh_(2 )by glucosidase from Terrabacter ginsenosidimutans.The best conditions of reaction are at 40 ℃ and a pH value of 7.0-8.0.The process is simpler,higher efficient,with less by-product,lower cost than chemical catalysis.It is more applicable for industrial production.
引文
[1]傅荣昭,刘立辉,张贵慰,等.一种酶催化合成人参皂苷Rh2的方法.CN108064309,May,22,2018.
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[7]孙晓雨,吕国忠,谢明杰,等.人参皂苷糖苷水解酶高产菌株CGMCC No.4316发酵工艺[J].食品与发酵工业,2014,40(6):85-90.
[8]Ma X Q,Xiao H B,Liang X M.Identification of Ginsenosides in Panax Quinquefolium by LC-MS[J].Chromatographia,2006,64,31-36.
[9]Liu C Y,Jin Y H,Yu H S,et al.Biotransformation pathway and kinetics of the hydrolysis of the 3-O-and 20-O-multi-glucosides of PPD-type ginsenosides by ginsenosidase type I[J].Process Biochemistry,2014,49:813-820.
[10]Yu H S,Liu H,Zhang C Z,et al.Purification and characterization of gypenoside-α-L-rhamnosidase hydrolyzing gypenoside-5 into ginsenoside Rd[J].Process Biochemistry,2004,39:861-867.
[11]Wang D M,Yu H S,Song J G,et al.Enzyme kinetics of ginsenosidase type IV hydrolyzing 6-O-multi-glycosides of protopanaxatriol type ginsenosides[J].Process Biochemistry,2012,47:133-138.
[12]Kyung C S,Deok K O.Characterization of a novel recombinant β-glucosidase from Sphingopyxis alaskensis that specifically hydrolyzes the outer glucose at the C-3 position in protopanaxadiol-type ginsenosides[J].Journal of Biotechnology,2014,172:30-37.
[2]Yang W Z,Hu Y,Wu W Y,et al.Saponins in the genus Panax L.(Araliaceae):A systematic review of their chemical diversity[J].Phytochemistry(Elsevier),2014,106:7-24.
[3]Lee C H,Kim J H.A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases[J].Journal of Ginseng Research,2014,38(3):161-166.
[4]Wen X,Zhang H D,ZHAO L,et al.Ginsenoside Rh2 differentially mediates microrna expression to prevent chemoresistance of breast cancer[J].Asian Pacific Journal of Cancer Prevention:APJCP,2015,16(3):1105-1109.
[5]Yang X D,Yang Y Y,Ouuang D S,et al.A review of biotransformation and pharmacology of ginsenoside compound K[J].Fitoterapia,2015,100:208-220.
[6]姬庆,郜玉钢,赵岩,等.酶法转化制备人参皂苷单体的研究进展[J].中草药,2014(22):3356-3360.
[7]孙晓雨,吕国忠,谢明杰,等.人参皂苷糖苷水解酶高产菌株CGMCC No.4316发酵工艺[J].食品与发酵工业,2014,40(6):85-90.
[8]Ma X Q,Xiao H B,Liang X M.Identification of Ginsenosides in Panax Quinquefolium by LC-MS[J].Chromatographia,2006,64,31-36.
[9]Liu C Y,Jin Y H,Yu H S,et al.Biotransformation pathway and kinetics of the hydrolysis of the 3-O-and 20-O-multi-glucosides of PPD-type ginsenosides by ginsenosidase type I[J].Process Biochemistry,2014,49:813-820.
[10]Yu H S,Liu H,Zhang C Z,et al.Purification and characterization of gypenoside-α-L-rhamnosidase hydrolyzing gypenoside-5 into ginsenoside Rd[J].Process Biochemistry,2004,39:861-867.
[11]Wang D M,Yu H S,Song J G,et al.Enzyme kinetics of ginsenosidase type IV hydrolyzing 6-O-multi-glycosides of protopanaxatriol type ginsenosides[J].Process Biochemistry,2012,47:133-138.
[12]Kyung C S,Deok K O.Characterization of a novel recombinant β-glucosidase from Sphingopyxis alaskensis that specifically hydrolyzes the outer glucose at the C-3 position in protopanaxadiol-type ginsenosides[J].Journal of Biotechnology,2014,172:30-37.
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