维生素D与IVF-ET结局的相关性分析
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摘要
目的研究体内维生素D水平与体外受精-胚胎移植(In vitro fertilization and embryo transfer,IVFET)结局的关联性。方法回顾性分析2016年1月-2017年12月在我院生殖医学科接受体外受精-胚胎移植的妇女235名,检测其血清中25-羟基维生素D水平。根据结果将病人分成维生素D缺乏组(<20ng/ml)和不缺乏组(≥20ng/ml),比较两组IVF结局,主要观察指标为平均获卵数、正常受精卵数、卵裂数、胚胎移植数、生化妊娠率、临床妊娠率和妊娠结局。结果235名研究对象中,维生素D缺乏组(53.2%)和不缺乏组(46.8%)相比,维生素D缺乏组临床妊娠率降低,差异有统计学意义(P<0.05),其他指标差异无统计学意义。结论维生素D缺乏影响体外受精-胚胎移植的临床妊娠结果。
Objective:The study investigated whether there is a correlation between levels of vitamin D and the results of IVF-ET(In vitro fertilization and embryo transfer). Methods:A retrospective analysis was conducted among 235 women undergoing in vitro fertilization-embryo transfer in reproductive medicine of our hospital from January 2016 to December 2017,The 25-hydroxy vitamin D levels were measured of serum. According to the results,the patients were divided into vitamin D deficiency group(<20 ng/ml)and non-deficiency group(≥20 ng/ml).The main observation index was mean number of the oocytes retrieved,fertilized eggs,cleavages and embryos transferred,fertilization rates and clinical outcomes were compared between the 2 groups. Results:Among 235 subjects,the vitamin D deficiency group(53.2%)compared with the non-deficient group(46.8%),clinical pregnancy rate of vitamin D deficiency group decreased,the difference was statistically significant(P<0.05). No significant difference in other indicators. Conclusion:Vitamin D deficiency affects clinical pregnancy outcomes of in vitro fertilization-embryo transfer.
Objective:The study investigated whether there is a correlation between levels of vitamin D and the results of IVF-ET(In vitro fertilization and embryo transfer). Methods:A retrospective analysis was conducted among 235 women undergoing in vitro fertilization-embryo transfer in reproductive medicine of our hospital from January 2016 to December 2017,The 25-hydroxy vitamin D levels were measured of serum. According to the results,the patients were divided into vitamin D deficiency group(<20 ng/ml)and non-deficiency group(≥20 ng/ml).The main observation index was mean number of the oocytes retrieved,fertilized eggs,cleavages and embryos transferred,fertilization rates and clinical outcomes were compared between the 2 groups. Results:Among 235 subjects,the vitamin D deficiency group(53.2%)compared with the non-deficient group(46.8%),clinical pregnancy rate of vitamin D deficiency group decreased,the difference was statistically significant(P<0.05). No significant difference in other indicators. Conclusion:Vitamin D deficiency affects clinical pregnancy outcomes of in vitro fertilization-embryo transfer.
引文
[1]Holick MF,Binkley NC,Bischoff-Ferrari HA,et al. Evalu-ation,treatment,and prevention of vitamin D deficiency:an Endocrine Society clinical practice guideline[J].J Clin Endo-crinol Metab,2011,96(7):1911-1930.
[2]Zec I,Tislaricmedenjak D,Megla ZB,et al. Anti-Mullerian hormone:a unique biochemical marker of gonadal development and fertility in humans[J].Biochem Med(Zagreb),2011,21(3):219-230.
[3]Ozkan S,Jindal S,Greenseid K,et al. Replete vitamin D stores predict reproductive success following in vitro fertilization[J].Fertil Steril,2010,94(4):1314-1319.
[4]Dennis NA,Houghton LA,Jones GT,et al. The level of serum anti-Mullerian hormone correlates with vitamin D status in men and women but not in boys[J].J Clin Endocrinol Metab,2012,97(7):2450-2455.
[5]He C,Lin Z,Robb SW,et al. Serum Vitamin D Levels and Polycystic Ovary syndrome:A Systematic Review and MetaAnalysis[J].Nutrients,2015,7(6):4555-4577.
[6]Merhi Z,Doswell A,Krebs K,et al. Vitamin D alters genes involved in follicular development and steroidogenesis in human cumulus granulosa cells[J].J Clin Endocrinol Metab,2014,99(6):E1137-1145.
[7]Irani M,Merhi Z. Role of vitamin D in ovarian physiology and its implication in reproduction:a systematic review[J].Fertil Steril,2014,102(2):460-468.
[8]Aquila S,Guido C,Perrotta I,et al. Human sperm anatomy:ultrastructural localization of 1alpha,25-dihydroxyvitamin D receptor and its possible role in the human male gamete[J].J Anat,2008,213(5):555-564.
[9]Kinuta K,Tanaka H,Moriwake T et al. Vitamin D is an important factor in Estrogen Biosynthesis of Both Female and Male Gonads[J].Endocrinology,2000,141(4):1317-1324.
[10] Garbedina K,Boggild M,et al. Effect of Vitamin D status on clinical pregnancy rates following in vitro fertilization[J].CMAJ Open,2013,1(2):77-82.
[11] Franasiak JM,Molinaro TA,Dubell EK,et al. Vitamin D levels do not affact IVF outcomes following the transfer of euploid blastocysts[J].Am J Obstet Gynecol,2015,212(3):315-321.
[12] Vilarino FL,Bianco B,Lerner TG,et al. Analysis of vitamin D receptor gene polymorphisms in women with and without endometriosis[J].Hum Immunol,2011,72(4):359-363.
[2]Zec I,Tislaricmedenjak D,Megla ZB,et al. Anti-Mullerian hormone:a unique biochemical marker of gonadal development and fertility in humans[J].Biochem Med(Zagreb),2011,21(3):219-230.
[3]Ozkan S,Jindal S,Greenseid K,et al. Replete vitamin D stores predict reproductive success following in vitro fertilization[J].Fertil Steril,2010,94(4):1314-1319.
[4]Dennis NA,Houghton LA,Jones GT,et al. The level of serum anti-Mullerian hormone correlates with vitamin D status in men and women but not in boys[J].J Clin Endocrinol Metab,2012,97(7):2450-2455.
[5]He C,Lin Z,Robb SW,et al. Serum Vitamin D Levels and Polycystic Ovary syndrome:A Systematic Review and MetaAnalysis[J].Nutrients,2015,7(6):4555-4577.
[6]Merhi Z,Doswell A,Krebs K,et al. Vitamin D alters genes involved in follicular development and steroidogenesis in human cumulus granulosa cells[J].J Clin Endocrinol Metab,2014,99(6):E1137-1145.
[7]Irani M,Merhi Z. Role of vitamin D in ovarian physiology and its implication in reproduction:a systematic review[J].Fertil Steril,2014,102(2):460-468.
[8]Aquila S,Guido C,Perrotta I,et al. Human sperm anatomy:ultrastructural localization of 1alpha,25-dihydroxyvitamin D receptor and its possible role in the human male gamete[J].J Anat,2008,213(5):555-564.
[9]Kinuta K,Tanaka H,Moriwake T et al. Vitamin D is an important factor in Estrogen Biosynthesis of Both Female and Male Gonads[J].Endocrinology,2000,141(4):1317-1324.
[10] Garbedina K,Boggild M,et al. Effect of Vitamin D status on clinical pregnancy rates following in vitro fertilization[J].CMAJ Open,2013,1(2):77-82.
[11] Franasiak JM,Molinaro TA,Dubell EK,et al. Vitamin D levels do not affact IVF outcomes following the transfer of euploid blastocysts[J].Am J Obstet Gynecol,2015,212(3):315-321.
[12] Vilarino FL,Bianco B,Lerner TG,et al. Analysis of vitamin D receptor gene polymorphisms in women with and without endometriosis[J].Hum Immunol,2011,72(4):359-363.