温度外扰的二维相关荧光谱鉴别4种食用植物油的研究
|
摘要
采用F97S荧光分光光度计结合温度扰动下的二维相关荧光光谱技术,对芝麻油、棕榈油、菜籽油和油茶籽油,进行了快速无损的识别方法的研究,并以市售4种植物油样品进行验证试验。结果表明,4种食用植物油样加热后的一维荧光发射光谱谱图在出峰数目、位置以及峰强度上都略有差别,而结合二维相关荧光光谱对其进行进一步的研究时,其谱图在自动峰和交叉峰的出峰数目、位置以及交叉峰正负情况上区别更为清晰直观。表明以温度为外扰,二维相关荧光光谱能够对食用植物油进行有效区分。
Adopted F97 S fluorospectro photometer with the two- dimensional correlation fluorescence under the temperature disturbance to conduct study of the quick and non- destructive identification method on sesame oil,palm oil,rapeseed oil and camellia seed oil Furthermore,use 4 kinds of commercially available vegetable oil samples for validation test. The results showed that,on the emission fluorescence spectra,the characteristic absorption peak of each vegetable oil varied in numbers,locations and intensities of peaks. Furthermore,when took the further study with the two- dimensional correlation fluorescence,the peak numbers,locations as well as the positive and negative situations of the cross- peaks of its atlas on auto- peaks and cross- peaks can be identified more clear and visualized. It showed that took the temperature as the disturbance,the edible vegetable oils can be effectively identified by the two- dimensional correlation fluorescence.
Adopted F97 S fluorospectro photometer with the two- dimensional correlation fluorescence under the temperature disturbance to conduct study of the quick and non- destructive identification method on sesame oil,palm oil,rapeseed oil and camellia seed oil Furthermore,use 4 kinds of commercially available vegetable oil samples for validation test. The results showed that,on the emission fluorescence spectra,the characteristic absorption peak of each vegetable oil varied in numbers,locations and intensities of peaks. Furthermore,when took the further study with the two- dimensional correlation fluorescence,the peak numbers,locations as well as the positive and negative situations of the cross- peaks of its atlas on auto- peaks and cross- peaks can be identified more clear and visualized. It showed that took the temperature as the disturbance,the edible vegetable oils can be effectively identified by the two- dimensional correlation fluorescence.
引文
[1]Noda I.Advances in two-dimensional correlation spectroscopy[J].Vibrational Spectroscopy,2004,36(2):143-165
[2]Noda I.Handbook of vibrational spectroscopy[M],2002,3:2123-2124
[3]Noda I,Dowrey A E,Marcott C,et al.Application spectroscopy[M],2000,54:236A-248A
[4]田萍,陈斌,陆道礼,等.二维相关荧光光谱鉴别4种食用植物油种类的研究[J].食品安全质量检测学报,2011,2(6):294-298
[5]雷猛,冯新沪.广义二维相关光谱技术及其应用[J].光谱实验室,2008,25(5):996-1002
[6]赵守敬.多种荧光光谱分析技术在食用植物油品种识别中的应用[D].2012,06
[7]张咏,程瑶,闫雨桐,等.几种常见食用油加热后荧光光谱特性变化的研究[J].激光技术,2013,37(1):109-113
[8]Bertran E,Coello J,Iturriaga H,et al.Determination of olive oil free fatty acid by fourier transform infrared spectroscopy[J].Journal of the American Oil Chemists Society,1999,76(8):611-616
[9]许金钩,王尊本.荧光分析法[M].科学出版社,1990
[10]Park Y W,Chang P S,Lee J H.Application of triacylglycerol and fatty acid analyses to discriminate blended sesame oil with soybean oil[J].Food Chemistry,2010,123:377-383
[11]彭翠红,王少玲,徐永群,等.荧光光谱法快速鉴别花生油、芝麻油和调和油[J].光谱实验室,2012,29(2):750-756
[12]Chen Bin,Tian Ping,Lu Daoli,et al.Feasibility study of discriminating edible vegetable oils by 2D-NIR[J].Analytical Methods,2012,4:4310-4315
[13]杨春英,刘学铭,陈志毅,等.食用植物油流变学特性研究[J].中国粮油学报,2013,28(8):24-27
[14]杨仁杰,,刘蓉,徐可欣.二维相关光谱结合偏最小二乘法测定牛奶中的掺杂尿素[J].农业工程学报2012,28(6):259-263.
[2]Noda I.Handbook of vibrational spectroscopy[M],2002,3:2123-2124
[3]Noda I,Dowrey A E,Marcott C,et al.Application spectroscopy[M],2000,54:236A-248A
[4]田萍,陈斌,陆道礼,等.二维相关荧光光谱鉴别4种食用植物油种类的研究[J].食品安全质量检测学报,2011,2(6):294-298
[5]雷猛,冯新沪.广义二维相关光谱技术及其应用[J].光谱实验室,2008,25(5):996-1002
[6]赵守敬.多种荧光光谱分析技术在食用植物油品种识别中的应用[D].2012,06
[7]张咏,程瑶,闫雨桐,等.几种常见食用油加热后荧光光谱特性变化的研究[J].激光技术,2013,37(1):109-113
[8]Bertran E,Coello J,Iturriaga H,et al.Determination of olive oil free fatty acid by fourier transform infrared spectroscopy[J].Journal of the American Oil Chemists Society,1999,76(8):611-616
[9]许金钩,王尊本.荧光分析法[M].科学出版社,1990
[10]Park Y W,Chang P S,Lee J H.Application of triacylglycerol and fatty acid analyses to discriminate blended sesame oil with soybean oil[J].Food Chemistry,2010,123:377-383
[11]彭翠红,王少玲,徐永群,等.荧光光谱法快速鉴别花生油、芝麻油和调和油[J].光谱实验室,2012,29(2):750-756
[12]Chen Bin,Tian Ping,Lu Daoli,et al.Feasibility study of discriminating edible vegetable oils by 2D-NIR[J].Analytical Methods,2012,4:4310-4315
[13]杨春英,刘学铭,陈志毅,等.食用植物油流变学特性研究[J].中国粮油学报,2013,28(8):24-27
[14]杨仁杰,,刘蓉,徐可欣.二维相关光谱结合偏最小二乘法测定牛奶中的掺杂尿素[J].农业工程学报2012,28(6):259-263.