中国近海铜藻ITS与coxⅠ序列相似性分析
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摘要
本研究分别对2016和2017年中国近海10个铜藻(Sargassumhorneri)漂浮地理种群以及3个定生种群的51个采集样本进行了ITS和coxⅠ序列分析及相似性比对。结果显示,51个样本的coxⅠ序列完全一致,ITS序列存在2个变异位点,按基因型的异同可分为4个类型,其中大连龙王塘、烟台大钦岛和南隍城岛的漂浮铜藻基因型相同,青岛金沙滩漂浮型、烟台大钦岛和大连獐子岛的定生铜藻基因型相同,威海俚岛和温州洞头的漂浮铜藻基因型相同,青岛雕塑园、王哥庄、大珠山、威海乳山的漂浮型和枸杞岛定生铜藻基因型相同,而同一种群内部即便是不同年份的个体间基因型并没有差异。基于ITS序列构建的系统树显示,来自中国的所有铜藻样本聚为一支,与来自韩国的铜藻样本有一定的遗传距离。以上结果说明,我国近海漂浮和定生铜藻的不同地理株间ITS和coxⅠ的遗传变异水平较低,漂浮铜藻可能具有不同的来源,为进一步探明中国近海海域铜藻的分子遗传背景提供依据。
Large-scale green tides have broken out annually in the Yellow Sea since 2007, which, upon twinning with floating Sargassum horneri, became a new hazard and increased the difficulty of preventing and controlling the green tide in Shandong Peninsula. In this study, the similarity of ITS and cox I sequences were analyzed using S. horneri samples collected from ten free-floating populations and three benthic populations in 2016 and 2017 along the offshore of China. The results showed that the cox I sequences of 51 samples were consistent, while there were two variable nucleotide sites within the ITSsequence. Based on genotypic differences, S. horneri samples could be sorted into four categories, among which the genotype of free-floating samples from Dalian Longwangtang, Daqin Island, and Nanhuangcheng Island of Yantai were identical. Free-floating samples from the Golden Beach of Qingdao and benthic samples from Daqin Island of Yantai and Zhangzi Island of Dalian were identical; free-floating samples from Lidao of Weihai and Dongtou of Wenzhou were identical; and free-floating samples from the Sculpture Garden, Wanggezhuang, Dazhushan of Qingdao, Rushan of Weihai, and benthic samples from Gouqi Island were identical. However, there was no difference in genotype among individuals within the same population, even among those occurring in different years. A phylogenetic tree based on ITS sequences of S. horneri showed that samples from China were clustered together; however, there was some genetic distance from the Korean samples. It is possible that the genetic variation of ITS and cox I regions is low in different coastal strains of S. horneri, and that drifting population could be of different origins. These findings will provide a basis for further understanding of the molecular genetic background of S. horneri along the offshore of China.
Large-scale green tides have broken out annually in the Yellow Sea since 2007, which, upon twinning with floating Sargassum horneri, became a new hazard and increased the difficulty of preventing and controlling the green tide in Shandong Peninsula. In this study, the similarity of ITS and cox I sequences were analyzed using S. horneri samples collected from ten free-floating populations and three benthic populations in 2016 and 2017 along the offshore of China. The results showed that the cox I sequences of 51 samples were consistent, while there were two variable nucleotide sites within the ITSsequence. Based on genotypic differences, S. horneri samples could be sorted into four categories, among which the genotype of free-floating samples from Dalian Longwangtang, Daqin Island, and Nanhuangcheng Island of Yantai were identical. Free-floating samples from the Golden Beach of Qingdao and benthic samples from Daqin Island of Yantai and Zhangzi Island of Dalian were identical; free-floating samples from Lidao of Weihai and Dongtou of Wenzhou were identical; and free-floating samples from the Sculpture Garden, Wanggezhuang, Dazhushan of Qingdao, Rushan of Weihai, and benthic samples from Gouqi Island were identical. However, there was no difference in genotype among individuals within the same population, even among those occurring in different years. A phylogenetic tree based on ITS sequences of S. horneri showed that samples from China were clustered together; however, there was some genetic distance from the Korean samples. It is possible that the genetic variation of ITS and cox I regions is low in different coastal strains of S. horneri, and that drifting population could be of different origins. These findings will provide a basis for further understanding of the molecular genetic background of S. horneri along the offshore of China.
引文
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Kucera H,Saunders GW.Assigning morphological variants of Fucus(Fucales,Phaeophyceae)in Canadian waters to recognized species using DNA barcoding.Botany-Botanique,2008,86(9):1065-1079
Lane CE,Lindstrom SC,Saunders GW.A molecular assessment of northeast Pacific Alaria species(Laminariales,Phaeophyceae)with reference to the utility of DNAbarcoding.Molecular Phylogenetics and Evolution,2007,44(2):634-648
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Sun JZ,Zhuang DG,Chen WD,et al.Studies on sexual reproduction and seedling production of the brown alga Sargassum horneri.South China Fisheries Science,2008b,4(2):6-14[孙建璋,庄定根,陈万东,等.铜藻Sargassum horneri繁殖生物学及种苗培育研究.南方水产,2008b,4(2):6-14]
Sun JZ,Zhuang DG,Sun QH,et al.Artificial cultivation trials of Sargassum horneri at Nanji islands of China.South China Fisheries Science,2009,5(6):41-46[孙建璋,庄定根,孙庆海,等.铜藻人工栽培的初步研究.南方水产,2009,5(6):41-46]
Wang GC,Tang XX,He PM,et al.Progress of studies on the responses of the key physiological processes including photosynthese in Ulva prolifera O.F.Müller to environmental factors.Plant Physiology Journal,2016,52(11):1627-1636[王广策,唐学玺,何培民,等.浒苔光合作用等关键生理过程对环境因子响应途径的研究进展.植物生理学报,2016,52(11):1627-1636]
Wang WJ,Wang FJ,Chen SL,et al.PCR amplification and sequence analysis of ITS regions of Enteromorpha prolifera.Marine Fisheries Research,2008,29(5):124-129[汪文俊,王飞久,陈松林,等.浒苔ITS区的扩增和分析.海洋水产研究,2008,29(5):124-129]
White TJ,Bruns T,Lee S,et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.PCR Protocols,1990,315-322
Yoshida T,Stiger V,Horiguchi T.Sargassum boreale sp.nov.(Fucales,Phaeophyceae)from Hokkaido,Japan.Phycological Research,2000,48(3):125-131
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Zhan DM,Wang XY,Xin ML,et al.Nutritional constituents of three kinds of Sargassum.Journal of Guangxi Academy of Sciences,2016,32(3):221-225[詹冬梅,王翔宇,辛美丽,等.三种马尾藻的营养组成分析.广西科学院学报,2016,32(3):221-225]
Cai YC,Sun B,Ma JH,et al.Molecular identification of floating Sargassum horneri in the southern Yellow Sea.Marine Fisheries,2014,36(2):102-106[蔡永超,孙彬,马家海,等.我国南黄海海域漂浮铜藻的分子生物学鉴定.海洋渔业,2014,36(2):102-106]
Cai YF,Zhang P,Wang TG,et al.Genetic analysis based on 5.8Sr DNA-ITS sequence of Sargassum horneri(Turner)C.Agardh in the coastal areas of Zhejiang Province,China.Journal of Tropical and Subtropical Botany,2013,21(2):147-154[蔡一凡,张鹏,王铁杆,等.基于5.8Sr DNA-ITS序列的我国浙江沿海铜藻群体遗传多样性分析.热带亚热带植物学报,2013,21(2):147-154]
Chen J,Wang YC,Yu QR,et al.Molecular phylogenetic analysis of floating Sargassum horneri associated with green tides in coastal area of Qingdao.Journal of Biology,2016,33(1):39-42[陈军,王寅初,余秋2),等.绿潮暴发期间我国青岛漂浮铜藻的分子鉴定.生物学杂志,2016,33(1):39-42]
Hu CM.A novel ocean color index to detect floating algae in the global oceans.Remote Sensing of Environment,2009,113(10):2118-2129
Hu ZM,Uwai S,Yu SH,et al.Phylogeographic heterogeneity of the brown macroalga Sargassum horneri(Fucaceae)in the northwestern pacific in relation to late Pleistocene glaciation and tectonic configurations.Molecular Ecology,2011,20(18):3984-3909
Jin S,Han Z,Liu Y.A remote sensing method for discriminating Ulva prolifra and Sargassum.Remote Sensing Information,2016,31(2):44-48[金松,韩震,刘瑜.一种区分浒苔和马尾藻的遥感方法.遥感信息,2016,31(2):44-48]
Komatsu T,Matsunaga D,Mikami A,et al.Abundance of drifting seaweeds in eastern East China Sea.Journal of Applied Phycology,2008,20(5):801-809
Komatsu T,Tatsukawa K,Filippi JB,et al.Distribution of drifting seaweeds in eastern East China Sea.Journal of Marine Systems,2007,67(3-4):245-252
Kucera H,Saunders GW.Assigning morphological variants of Fucus(Fucales,Phaeophyceae)in Canadian waters to recognized species using DNA barcoding.Botany-Botanique,2008,86(9):1065-1079
Lane CE,Lindstrom SC,Saunders GW.A molecular assessment of northeast Pacific Alaria species(Laminariales,Phaeophyceae)with reference to the utility of DNAbarcoding.Molecular Phylogenetics and Evolution,2007,44(2):634-648
Liang JX,Pang CG,Bai XZ.Different methods for computation of wind-induced currents in southern Yellow Sea in summer.Marine Science,2005,29(9):60-64,75[梁兼霞,庞重光,白学志.夏季南黄海风漂流的不同计算方法的对比分析.海洋科学,2005,29(9):60-64,75]
Liu F,Pang SJ.Research advances on green tides in the yellow sea.Advances in Marine Science,2012,30(3):441-449[刘峰,逄少军.黄海浒苔绿潮及其溯源研究进展.海洋科学进展,2012,30(3):441-449]
Liu L,Heinrich M,Myers S,et al.Towards a better understanding of medicinal uses of the brown seaweed Sargassum in traditional Chinese medicine:A phytochemical and pharmacological review.Journal of Ethnopharmacology,2012,142(3):591-619
Mcdevit DC,Saunders GW.On the utility of DNA barcoding for species differentiation among brown macroalgae(Phaeophyceae)including a novel extraction protocol.Phycological Research,2009,57(2):131-141
Su L,Shan TF,Pang SJ,et al.Analyses of the genetic structure of Sargassum horneri in the Yellow Sea:Implications of the temporal and spatial relations among floating and benthic populations.Journal of Applied Phycology,2018,30(2):1417-1424
Sun JZ,Chen WD,Zhuang DG,et al.In situ ecological studies of the subtidal brown alga Sargasssum horneri at Nanji Island of China.South China Fisheries Science,2008a,4(3):58-63[孙建璋,陈万东,庄定根,等.中国南麂列岛铜藻Sargassum horneri实地生态学的初步研究.南方水产,2008a,4(3):58-63]
Sun JZ,Zhuang DG,Chen WD,et al.Studies on sexual reproduction and seedling production of the brown alga Sargassum horneri.South China Fisheries Science,2008b,4(2):6-14[孙建璋,庄定根,陈万东,等.铜藻Sargassum horneri繁殖生物学及种苗培育研究.南方水产,2008b,4(2):6-14]
Sun JZ,Zhuang DG,Sun QH,et al.Artificial cultivation trials of Sargassum horneri at Nanji islands of China.South China Fisheries Science,2009,5(6):41-46[孙建璋,庄定根,孙庆海,等.铜藻人工栽培的初步研究.南方水产,2009,5(6):41-46]
Wang GC,Tang XX,He PM,et al.Progress of studies on the responses of the key physiological processes including photosynthese in Ulva prolifera O.F.Müller to environmental factors.Plant Physiology Journal,2016,52(11):1627-1636[王广策,唐学玺,何培民,等.浒苔光合作用等关键生理过程对环境因子响应途径的研究进展.植物生理学报,2016,52(11):1627-1636]
Wang WJ,Wang FJ,Chen SL,et al.PCR amplification and sequence analysis of ITS regions of Enteromorpha prolifera.Marine Fisheries Research,2008,29(5):124-129[汪文俊,王飞久,陈松林,等.浒苔ITS区的扩增和分析.海洋水产研究,2008,29(5):124-129]
White TJ,Bruns T,Lee S,et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.PCR Protocols,1990,315-322
Yoshida T,Stiger V,Horiguchi T.Sargassum boreale sp.nov.(Fucales,Phaeophyceae)from Hokkaido,Japan.Phycological Research,2000,48(3):125-131
Zeng CK,Lu BR.Marine algal flora of China,Volume 3Phaeophyta,No.2 Fucales.Beijing:Science Press,2000,43-44[曾呈奎,陆保仁.中国海藻志第三卷褐藻门第二册墨角藻目.北京:科学出版社,2000,43-44]
Zhan DM,Wang XY,Xin ML,et al.Nutritional constituents of three kinds of Sargassum.Journal of Guangxi Academy of Sciences,2016,32(3):221-225[詹冬梅,王翔宇,辛美丽,等.三种马尾藻的营养组成分析.广西科学院学报,2016,32(3):221-225]
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