枸杞蜜的抗氧化活性及其对DNA氧化损伤的保护作用
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摘要
以青海枸杞蜜为研究对象,在测定其总酚、总黄酮含量和抗氧化活性基础上,采用彗星电泳技术,研究了枸杞蜜对羟基自由基诱导的小鼠淋巴细胞DNA氧化损伤的保护作用。结果表明,枸杞蜜不仅能够有效地清除自由基,而且具有较强的抗氧化活性,枸杞蜜的抗氧化活性与其总酚含量有关(p<0.05),Fe~(2+)络合力与其总黄酮含量相关(p<0.01)。彗星电泳结果表明,枸杞蜜能够显著降低羟基自由基诱导的小鼠淋巴细胞DNA的氧化损伤,与模型组相比,加入枸杞蜜的保护组彗星尾部DNA比例、尾矩(TM)和Olive尾矩(OTM)显著降低(p<0.05),且与枸杞蜜浓度存在明显的剂量-效应关系,线性回归方程决定系数依次为:R~2=0.9775,R~2=0.9341,R~2=0.9425。本文的研究结果将为枸杞蜜功能性食品的开发利用提供依据。
To study antioxidant activity of medlar honey,the total phenolic contents,total flavonoids contents and antioxidant activity in vitro of medlar honey from Qinghai province were determined. Protective effect of medlar honey on the oxidative damage of DNA induced by hydroxyl radicals was examined by comet electrophoresis. The results showed that medlar honey possessed capability to effectively remove free radicals and strong antioxidant activities. The antioxidant activity of medlar honey was related to its total phenol contents(p<0.05),while the ferrous ion-chelating activity of medlar honey correlated with it's total flavonoids contents. The results of comet electrophoresis showed that the DNA oxidant damage of mice lymphocytes induced by hydroxyl radicals was prevented by medlar honey. Compared to model group,the percent of DNA in the comet tail,tail moment and olive tail moment of experience group all significantly reduced(p<0.05)in a dose-dependent manner. The determinant coefficient of linear regression equation between the percent of DNA in the comet tail,tail moment,olive tail moment and concentration of medlar honey were R~2=0.9775,R~2=0.9341 and R~2=0.9425,respectively. This study would provide a basis for the development and utilization of medlar honey as functional food.
To study antioxidant activity of medlar honey,the total phenolic contents,total flavonoids contents and antioxidant activity in vitro of medlar honey from Qinghai province were determined. Protective effect of medlar honey on the oxidative damage of DNA induced by hydroxyl radicals was examined by comet electrophoresis. The results showed that medlar honey possessed capability to effectively remove free radicals and strong antioxidant activities. The antioxidant activity of medlar honey was related to its total phenol contents(p<0.05),while the ferrous ion-chelating activity of medlar honey correlated with it's total flavonoids contents. The results of comet electrophoresis showed that the DNA oxidant damage of mice lymphocytes induced by hydroxyl radicals was prevented by medlar honey. Compared to model group,the percent of DNA in the comet tail,tail moment and olive tail moment of experience group all significantly reduced(p<0.05)in a dose-dependent manner. The determinant coefficient of linear regression equation between the percent of DNA in the comet tail,tail moment,olive tail moment and concentration of medlar honey were R~2=0.9775,R~2=0.9341 and R~2=0.9425,respectively. This study would provide a basis for the development and utilization of medlar honey as functional food.
引文
[1]Spencer J P.Food for thought:The role of dietary flavonoids in enhancing human memory,learning and neuro-cognitive performance[J].Proceedings of the Nutrition Society,2008,67(2):238-52.
[2]Quiller G,á MéridaOrtega,Rothenberg S J,et al.Dietary flavonoids improve urinary arsenic elimination among Mexican women[J].Nutrition Research,2018,24(7):149.
[3]Maduni? J,Maduni? I V,Gajski G,et al.Apigenin:A dietary flavonoid with diverse anticancer properties[J].Cancer Letters,2018,413:11-22.
[4]Syed D N,Adhami V M,Khan N,et al.Exploring the molecular targets of dietary flavonoid fisetin in cancer[J].Seminars in Cancer Biology,2016,40-41:130-140.
[5]穆雪峰,徐响,孙丽萍,等.蜂蜜中酚类物质及其抗氧化活性研究进展[J].食品科学,2011,32(21):278-282.
[6]玄红专,吴玉厚,桑青,等.不同蜂蜜抗氧化活性的测定[J].食品研究与开发,2008,29(3):116-118.
[7]Péreziglesias J M,Ruiz d A C,Natale G S,et al.Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III-and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles(Anura,Hylidae)[J].Ecotoxicology and Environmental Safety,2017,142:503.
[8]赵健,李红丽,翟庆峰,等.核酸内切酶改良型彗星实验用于检测遗传毒物致DNA氧化损伤[J].中华预防医学杂志,2014,48(3):208-212.
[9]王远,贾歌,王萌,等.蜂蜜抗氧化活性研究进展[J].食品与发酵工业,2014,40(7):111-114.
[10]GB/T 23194-2008蜂蜜中植物花粉的测定方法[S].北京:中国标准出版社,2008.
[11]Zhou J,Li P,Cheng N,et al.Protective effects of buckwheat honey on DNA damage induced by hydroxyl radicals[J].Food & Chemical Toxicology an International Journal Published for the British Industrial Biological Research Association,2012,50(8):2766-2773.
[12]董蕊,丛海迪,郑毅男.单花蜂蜜多酚类物质的抗氧化活性[J].食品科学,2012,33(11):94-98.
[13]Benzie I F,Strain J J.The ferric reducing ability of plasma(FRAP)as a measure of“antioxidant power”:The FRAP assay[J].Analytical Biochemistry,1996,239(1):70-76.
[14]Liang Y,Cao W,Chen W J,et al.Simultaneous determination of four phenolic components in citrus honey by high performance liquid chromatography using electrochemical detection[J].Food Chemistry,2009,114(4):1537-1541.
[15]Singh N P,Mc Coy M T,Tice R R,et al.A simple technique for quantitation of low levels of DNA damage in individual cell[J].Experimental Cell Research,1988,175(1):184-191.
[16]赵立夫,徐云友,董蕊,等.单花蜜的化学成分研究进展[J].食品科学,2013,34(7):330-334.
[17]Tenore G C,Ritieni A,Campiglia P,et al.Nutraceutical potential of monofloral honeys produced by the Sicilian black honeybees(Apis mellifera ssp.sicula)[J].Food and Chemical Toxicology:An International Journal published for the British Industrial Biological Research Association,2012,50(6):1955-1961.
[18]曹炜,卢珂,陈卫军,等.不同种类蜂蜜抗氧化活性的研究[J].食品科学,2005,26(8):352-356.
[19]Shi F,Zhang P,Mao Y,et al.The nitroxide Tempo inhibits hydroxyl radical production from the Fenton-like reaction of iron(II)-citrate with hydrogen peroxide[J].Biochemical and Biophysical Research Communications,2017,483(1):159-164.
[20]Can Z,Yildiz O,Sahin H,et al.An investigation of Turkish honeys:Their physico-chemical properties,antioxidant capacities and phenolic profiles[J].Food Chemistry,2015,180:133-141.
[21]Habib H M,Meqbali F T A,Kamal H,et al.Bioactive components,antioxidant and DNA damage inhibitory activities of honeys from arid regions[J].Food Chemistry,2014,153(12):28-34.
[22]Wang X H,Gheldof N,Engeseth N J.Effect of processing and storage on antioxidant capacity of honey[J].Journal of Food Science,2004,69(2):6.
[23]Sun S,Chen W,Cao W,et al.Research on the chelation between quercetin and Cr(III)ion by Density Functional Theory(DFT)method[J].THEOCHEM,2008,860(1-3):40-44.
[24]Wang Y,Li D,Cheng N,et al.Antioxidant and hepatoprotective activity of vitex honey against paracetamol induced liver damage in mice[J].Food and Function,2015,6(7):2339.
[25]Xiao L,Luo G,Tang Y,et al.Quercetin and iron metabolism:What we know and what we need to know[J].Food and Chemical Toxicology,2018,114:190-203.
[26]张来军,李晓梅,陈永敢,等.重金属胁迫对罗非鱼血细胞DNA损伤的影响[J].海南热带海洋学院学报,2017,24(5):31-35.
[27]Cadet J,Wagner J R.Oxidatively generated base damage to cellular DNA by hydroxyl radical and one-electron oxidants:Similarities and differences[J].Archives of Biochemistry and Biophysics,2014,557(5):47-54.
[28]张华,周志钦,席万鹏.15种柑橘果实主要酚类物质的体外抗氧化活性比较[J].食品科学,2015,36(11):64-70.
[2]Quiller G,á MéridaOrtega,Rothenberg S J,et al.Dietary flavonoids improve urinary arsenic elimination among Mexican women[J].Nutrition Research,2018,24(7):149.
[3]Maduni? J,Maduni? I V,Gajski G,et al.Apigenin:A dietary flavonoid with diverse anticancer properties[J].Cancer Letters,2018,413:11-22.
[4]Syed D N,Adhami V M,Khan N,et al.Exploring the molecular targets of dietary flavonoid fisetin in cancer[J].Seminars in Cancer Biology,2016,40-41:130-140.
[5]穆雪峰,徐响,孙丽萍,等.蜂蜜中酚类物质及其抗氧化活性研究进展[J].食品科学,2011,32(21):278-282.
[6]玄红专,吴玉厚,桑青,等.不同蜂蜜抗氧化活性的测定[J].食品研究与开发,2008,29(3):116-118.
[7]Péreziglesias J M,Ruiz d A C,Natale G S,et al.Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III-and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles(Anura,Hylidae)[J].Ecotoxicology and Environmental Safety,2017,142:503.
[8]赵健,李红丽,翟庆峰,等.核酸内切酶改良型彗星实验用于检测遗传毒物致DNA氧化损伤[J].中华预防医学杂志,2014,48(3):208-212.
[9]王远,贾歌,王萌,等.蜂蜜抗氧化活性研究进展[J].食品与发酵工业,2014,40(7):111-114.
[10]GB/T 23194-2008蜂蜜中植物花粉的测定方法[S].北京:中国标准出版社,2008.
[11]Zhou J,Li P,Cheng N,et al.Protective effects of buckwheat honey on DNA damage induced by hydroxyl radicals[J].Food & Chemical Toxicology an International Journal Published for the British Industrial Biological Research Association,2012,50(8):2766-2773.
[12]董蕊,丛海迪,郑毅男.单花蜂蜜多酚类物质的抗氧化活性[J].食品科学,2012,33(11):94-98.
[13]Benzie I F,Strain J J.The ferric reducing ability of plasma(FRAP)as a measure of“antioxidant power”:The FRAP assay[J].Analytical Biochemistry,1996,239(1):70-76.
[14]Liang Y,Cao W,Chen W J,et al.Simultaneous determination of four phenolic components in citrus honey by high performance liquid chromatography using electrochemical detection[J].Food Chemistry,2009,114(4):1537-1541.
[15]Singh N P,Mc Coy M T,Tice R R,et al.A simple technique for quantitation of low levels of DNA damage in individual cell[J].Experimental Cell Research,1988,175(1):184-191.
[16]赵立夫,徐云友,董蕊,等.单花蜜的化学成分研究进展[J].食品科学,2013,34(7):330-334.
[17]Tenore G C,Ritieni A,Campiglia P,et al.Nutraceutical potential of monofloral honeys produced by the Sicilian black honeybees(Apis mellifera ssp.sicula)[J].Food and Chemical Toxicology:An International Journal published for the British Industrial Biological Research Association,2012,50(6):1955-1961.
[18]曹炜,卢珂,陈卫军,等.不同种类蜂蜜抗氧化活性的研究[J].食品科学,2005,26(8):352-356.
[19]Shi F,Zhang P,Mao Y,et al.The nitroxide Tempo inhibits hydroxyl radical production from the Fenton-like reaction of iron(II)-citrate with hydrogen peroxide[J].Biochemical and Biophysical Research Communications,2017,483(1):159-164.
[20]Can Z,Yildiz O,Sahin H,et al.An investigation of Turkish honeys:Their physico-chemical properties,antioxidant capacities and phenolic profiles[J].Food Chemistry,2015,180:133-141.
[21]Habib H M,Meqbali F T A,Kamal H,et al.Bioactive components,antioxidant and DNA damage inhibitory activities of honeys from arid regions[J].Food Chemistry,2014,153(12):28-34.
[22]Wang X H,Gheldof N,Engeseth N J.Effect of processing and storage on antioxidant capacity of honey[J].Journal of Food Science,2004,69(2):6.
[23]Sun S,Chen W,Cao W,et al.Research on the chelation between quercetin and Cr(III)ion by Density Functional Theory(DFT)method[J].THEOCHEM,2008,860(1-3):40-44.
[24]Wang Y,Li D,Cheng N,et al.Antioxidant and hepatoprotective activity of vitex honey against paracetamol induced liver damage in mice[J].Food and Function,2015,6(7):2339.
[25]Xiao L,Luo G,Tang Y,et al.Quercetin and iron metabolism:What we know and what we need to know[J].Food and Chemical Toxicology,2018,114:190-203.
[26]张来军,李晓梅,陈永敢,等.重金属胁迫对罗非鱼血细胞DNA损伤的影响[J].海南热带海洋学院学报,2017,24(5):31-35.
[27]Cadet J,Wagner J R.Oxidatively generated base damage to cellular DNA by hydroxyl radical and one-electron oxidants:Similarities and differences[J].Archives of Biochemistry and Biophysics,2014,557(5):47-54.
[28]张华,周志钦,席万鹏.15种柑橘果实主要酚类物质的体外抗氧化活性比较[J].食品科学,2015,36(11):64-70.