应用iTRAQ技术筛选卵巢过度刺激综合征差异表达蛋白
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摘要
目的:筛选卵巢过度刺激综合征的血清生物标记物。方法:选择临床明确诊断的卵巢过度刺激综合征患者(观察组)以及健康妇女(对照组)各20例,利用同位素标记相对和绝对定量(iTRAQ)技术检测其血清差异蛋白,并进行功能注释及生物信息分析,筛选出差异明显的候选蛋白。结果:与对照组相比,观察组获得64种血清差异蛋白。参与的主要生物学过程为炎症反应和免疫应答等,并获得与疾病相关的标志物5个。结论:炎症和免疫功能改变可能在该病证发生发展中发挥着关键性作用,5种蛋白可能作为卵巢过度刺激综合征的血清候选差异蛋白。
Objective:To identify and screen the potential biomarkers of women with ovarian hyperstimulation syndrome(OHSS).Methods:20 women with OHSS were selected in observation group,and another 20 health women were in control group.Identification and comparative quantification in protein samples of women in the two groups were performed using isobaric tags for relative and absolute qutification(iTRAQ),which used to analyze gene function cluster(GO),protein classification and path analysis(pathway).Results:Total of 64 serum differentially expressed proteins was identified by iTRAQ.The main biological processes of differential proteins were injury,immune response.Five candidate biomarkers associated with OHSS were found.Conclusion:Inflammation and immunologic function may play the key role in the development of OHSS,and five proteins can be used as candidate proteins related to OHSS.
Objective:To identify and screen the potential biomarkers of women with ovarian hyperstimulation syndrome(OHSS).Methods:20 women with OHSS were selected in observation group,and another 20 health women were in control group.Identification and comparative quantification in protein samples of women in the two groups were performed using isobaric tags for relative and absolute qutification(iTRAQ),which used to analyze gene function cluster(GO),protein classification and path analysis(pathway).Results:Total of 64 serum differentially expressed proteins was identified by iTRAQ.The main biological processes of differential proteins were injury,immune response.Five candidate biomarkers associated with OHSS were found.Conclusion:Inflammation and immunologic function may play the key role in the development of OHSS,and five proteins can be used as candidate proteins related to OHSS.
引文
[1]Delvigne A,Rozenberg S.Epidemiology and prevention of ovarian hyperstimulation Syndrome(OHSS):a review[J].Hum Reprod Update,2002,8(6):559-577.
[2]钱敏,刘建华.77例卵巢过度刺激综合征临床分析[J].上海交通大学学报(医学版),2017,37(4):538-541.
[3]张园,崔毓桂,刘嘉茵.重度卵巢过度刺激综合征患者妊娠结局[J].生殖医学杂志.2015,(10):869-872.
[4]蒋春艳,王成东,张海灵,等.iTRAQ定量蛋白质组学技术筛选唐氏综合征孕母血清差异蛋白[J].哈尔滨医科大学学报,2015,49(06):513-517,520.
[5]Http://www.Geneontology.org/(不符本刊著录格式,请修改)
[6]沈兰,李爱斌,曹景云.卵巢过度刺激综合征的防治进展[J].中华临床医师杂志:电子版.2013,(4):1712-1714.
[7]陈骊珠,马巍,张墨,等.应用iTRAQ技术筛选先天性心脏畸形胎儿母亲血清差异表达蛋白[J].中国医科大学学报,2017,(6):510-514.
[8]韩小东,贾天军,贾晓晖,等.甘露聚糖结合凝集素相关丝氨酸蛋白酶2(MASP-2)的CUB1、CUB2片段多克隆抗体的制备及鉴定[J].细胞与分子免疫学杂志,2015,(5):689-692.
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[10]王倩,陈慧昱,陆小铃,等.甘露糖结合凝集素相关丝氨酸蛋白酶2的研究进展[J].细胞与分子免疫学杂志,2015,(1):129-133.
[11]Orvieto R.Controlled ovarian hyperstimulation??an inflammatory state[J].Reproductive Sciences,2004,11(7):424-426.
[12]张晓慧,李光韬,张卓莉.C反应蛋白与超敏C反应蛋白的检测及其临床意义[J].中华临床免疫和变态反应杂志,2011,(1):74-79.
[13]Tomita S.C-reactive protein[J].Nihon Rinsho Japanese Journal of Clinical Medicine,1973,31(17):1896.
[14]Grossman L C,Michalakis K G,Browne H,et al.The pathophysiology of ovarian hyperstimulation syndrome:an unrecognized compartment syndrome[J].Fertility&Sterility,2010,94(4):1392-1398.
[15]马文静,毛吾兰·买买提依明,等.基于iTRAQ标记结合IPA生物分析平台筛选异常黏液质型阳痿病证大鼠血清蛋白质组学研究[J].中国男科学杂志,2016,30(12):3-9.
[16]Emberson J,Bennett D,Link E,et al.C-reactive protein concentration and the vascular benefits of statin therapy:an analysis of 20536patients in the Heart Protection Study[J].Lancet,2011,377(9764):469.
[17]杨呈浩,陈岷辉,丁昊.动脉瘤性蛛网膜下腔出血患者血清血管假性血友病因子、内皮素-1含量变化及与脑血管痉挛的关系[J].中风与神经疾病杂志,2016,(6):521-524.
[18]郭晔炳,柳景华.血管内皮功能的检测及临床意义[J].心肺血管病杂志,2009,(6):443-446.
[19]刘梅梅,丁慧,尹晓静,等.多囊卵巢综合征患者血清血管内皮生长因子、内皮抑素水平及对卵巢间质血流的影响研究[J].中国全科医学,2017,20(4):448-453.
[2]钱敏,刘建华.77例卵巢过度刺激综合征临床分析[J].上海交通大学学报(医学版),2017,37(4):538-541.
[3]张园,崔毓桂,刘嘉茵.重度卵巢过度刺激综合征患者妊娠结局[J].生殖医学杂志.2015,(10):869-872.
[4]蒋春艳,王成东,张海灵,等.iTRAQ定量蛋白质组学技术筛选唐氏综合征孕母血清差异蛋白[J].哈尔滨医科大学学报,2015,49(06):513-517,520.
[5]Http://www.Geneontology.org/(不符本刊著录格式,请修改)
[6]沈兰,李爱斌,曹景云.卵巢过度刺激综合征的防治进展[J].中华临床医师杂志:电子版.2013,(4):1712-1714.
[7]陈骊珠,马巍,张墨,等.应用iTRAQ技术筛选先天性心脏畸形胎儿母亲血清差异表达蛋白[J].中国医科大学学报,2017,(6):510-514.
[8]韩小东,贾天军,贾晓晖,等.甘露聚糖结合凝集素相关丝氨酸蛋白酶2(MASP-2)的CUB1、CUB2片段多克隆抗体的制备及鉴定[J].细胞与分子免疫学杂志,2015,(5):689-692.
[9]刘林林.甘露糖结合凝集素在IgA肾病中作用及其分子机制的初步研究[D].沈阳:中国医科大学,2013.
[10]王倩,陈慧昱,陆小铃,等.甘露糖结合凝集素相关丝氨酸蛋白酶2的研究进展[J].细胞与分子免疫学杂志,2015,(1):129-133.
[11]Orvieto R.Controlled ovarian hyperstimulation??an inflammatory state[J].Reproductive Sciences,2004,11(7):424-426.
[12]张晓慧,李光韬,张卓莉.C反应蛋白与超敏C反应蛋白的检测及其临床意义[J].中华临床免疫和变态反应杂志,2011,(1):74-79.
[13]Tomita S.C-reactive protein[J].Nihon Rinsho Japanese Journal of Clinical Medicine,1973,31(17):1896.
[14]Grossman L C,Michalakis K G,Browne H,et al.The pathophysiology of ovarian hyperstimulation syndrome:an unrecognized compartment syndrome[J].Fertility&Sterility,2010,94(4):1392-1398.
[15]马文静,毛吾兰·买买提依明,等.基于iTRAQ标记结合IPA生物分析平台筛选异常黏液质型阳痿病证大鼠血清蛋白质组学研究[J].中国男科学杂志,2016,30(12):3-9.
[16]Emberson J,Bennett D,Link E,et al.C-reactive protein concentration and the vascular benefits of statin therapy:an analysis of 20536patients in the Heart Protection Study[J].Lancet,2011,377(9764):469.
[17]杨呈浩,陈岷辉,丁昊.动脉瘤性蛛网膜下腔出血患者血清血管假性血友病因子、内皮素-1含量变化及与脑血管痉挛的关系[J].中风与神经疾病杂志,2016,(6):521-524.
[18]郭晔炳,柳景华.血管内皮功能的检测及临床意义[J].心肺血管病杂志,2009,(6):443-446.
[19]刘梅梅,丁慧,尹晓静,等.多囊卵巢综合征患者血清血管内皮生长因子、内皮抑素水平及对卵巢间质血流的影响研究[J].中国全科医学,2017,20(4):448-453.