2008年-2017年湖州市手足口病病原学特征分析
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摘要
目的了解湖州市手足口病病原学构成特征,为防控手足口病提供实验室数据。方法收集临床诊断为手足口病患者疱疹液、咽拭子等标本3 194份,用实时荧光定量聚合酶链反应(real-time PCR)检测并分型。结果 2008年-2017年从3 194份标本检出肠道病毒核酸阳性2 328份,阳性率为72.9%,其中肠道病毒71型(EV71)602份,阳性率为25.9%,柯萨奇病毒A组16型(CA16)570份,阳性率为24.5%。2009年-2011年手足口病病原体型别以EV71为优势毒株,2012年以EV71和CA16为优势毒株,2013年-2017年及2008年以其他肠道病毒为优势毒株。2016年-2017年从321份其他肠道病毒核酸阳性中检出柯萨奇病毒A组6型(CA6)96份,阳性率为29.9%,柯萨奇病毒A组10型(CA10)21份,阳性率为6.5%。结论十年间湖州市手足口病病原检出率较高,不同年份的各型别优势毒株呈动态出现。加强手足口病病原学监测,进一步明确本地区病原谱构成,掌握病毒流行规律,更好地预防和控制手足口病发生。
Objective To understand the etiological characteristics of hand, foot and mouth disease(HFMD) in Huzhou, so as to provide laboratory data for the prevention and treatment of HFMD. Methods A total of 3 194 specimens of herpes zoster and throat swabs were collected from patients with hand-foot-mouth disease. Real-time PCR was used to detect and classify the specimens. Results From 2008 to 2017, 2 328 positive samples of enteroviruses were detected from 3 194 samples, the positive rate was 72.9%, including 602 cases of enterovirus 71(EV71), the positive rate was 25.9%, and 570 cases of Coxsackie virus A group 16(CA16), with a positive rate of 24.5%. From 2009 to 2011, the pathogenic type of HFMD was dominated by EV71. EV71 and CA16 were the dominant strains in 2012 and other enteroviruses were the dominant strains in 2013-2017 and 2008. From 2016 to 2017, 96 copies of Coxsackie virus type A type 6(CA6) and 21 copies of Coxsackie virus A type 10(CA10) were detected from 527 positive samples with other enterovirus nucleic acids, with the positive rate of 29.9% and 6.5%. Conclusion The detection rate of pathogens of hand-foot-mouth disease in Huzhou City was relatively high during the ten years. The dominant virus strains with different types appeared dynamically in different years. It is necessary to strengthen the monitoring of etiology of hand-foot-mouth disease, further clarify the composition of pathogenic spectrum in the region, so as to better prevent and control the occurrence of hand-foot-mouth disease.
Objective To understand the etiological characteristics of hand, foot and mouth disease(HFMD) in Huzhou, so as to provide laboratory data for the prevention and treatment of HFMD. Methods A total of 3 194 specimens of herpes zoster and throat swabs were collected from patients with hand-foot-mouth disease. Real-time PCR was used to detect and classify the specimens. Results From 2008 to 2017, 2 328 positive samples of enteroviruses were detected from 3 194 samples, the positive rate was 72.9%, including 602 cases of enterovirus 71(EV71), the positive rate was 25.9%, and 570 cases of Coxsackie virus A group 16(CA16), with a positive rate of 24.5%. From 2009 to 2011, the pathogenic type of HFMD was dominated by EV71. EV71 and CA16 were the dominant strains in 2012 and other enteroviruses were the dominant strains in 2013-2017 and 2008. From 2016 to 2017, 96 copies of Coxsackie virus type A type 6(CA6) and 21 copies of Coxsackie virus A type 10(CA10) were detected from 527 positive samples with other enterovirus nucleic acids, with the positive rate of 29.9% and 6.5%. Conclusion The detection rate of pathogens of hand-foot-mouth disease in Huzhou City was relatively high during the ten years. The dominant virus strains with different types appeared dynamically in different years. It is necessary to strengthen the monitoring of etiology of hand-foot-mouth disease, further clarify the composition of pathogenic spectrum in the region, so as to better prevent and control the occurrence of hand-foot-mouth disease.
引文
[1] 中华人民共和国卫生部.手足口病预防控制指南(2009年版)[Z].2009-07-28.
[2] Li L,He Y,Yang H,et al.Genetic characteristics of human enterovirus 71 and coxsackievirus A16 People’s Republic of China[J].Clin Microbiol,2005,43(8):3835-3839.
[3] 任倩,马丽霞,张乐海.肠道病毒71致病机制的研究进展[J].国际流行病学传染病学杂志,2011,38(3):197-200.
[4] 王国玲,谭锦花,刘青连,等.广州市海珠区2008- 2012 年手足口病流行特征及病原学分析[J].中国卫生检验杂志,2014,24(5):688- 690.
[5] Yang F,Ren LL,Xiong ZH,et al.Enterovirus 71 outbreak inthe people’s republic of China in 2008[J].J Clin Microbiol,2009,47(7):2351-2352.
[6] 牛文柯,丁淑军,林艺,等.山东省2009-2010年手足口病死亡病例流行病学及临床特征分析[J].中国儿童保健杂志,2012,20(3):263-266.
[7] 白丽娜,王冰,王萍,等.2015年沈阳地区手足口病监测结果分析[J].中国卫生检验杂志,2016,26(20):2980-2981.
[8] 王永全,吉彦莉,曲梅.北京地区与手足口病相关的非EV714,非CA16 型肠道病毒的分子特征分析[J].国际病原学杂志,2011,18(6):75-79.
[9] 刘雅琼,袁伟,吕强.2009-2016年四川省手足口病流行病学和病原学特征分析[J].中华疾病控制杂志,2017,21(10):1052-1056.
[10] 王芬,方涛,祝绯飞,等.2010-2014年杭州市拱墅区手足口病疫情流行特征[J].疾病监测,2015,30(9):732-735.
[11] 李晓军,李芳,詹海丰.上海市普陀区2013 年手足口病病原学监测结果分析[J].海南医学,2015,26(5):755-757.
[12] 陈纯,肖新才,谢华萍,等.2008 -2013年广州市手足口病流行病学特征研究[J].疾病监测,2014,29(12):964-968.
[13] 王侃.锦州市2014-2016 年手足口病病原学研究[J].疾病监测与控制杂志,2017,11 (7):526-527.
[14] 丁克琴,陈奕,谢蕾,等.2011-2015 年宁波市手足口病流行病学与病原学特征分析[J].现代预防医学,2016,43(23):4251-4263.
[15] 何为涛,蒋丽娜,王东燕,等.广西2008-2015年手足口病流行病学特征及病原学监测分析[J].中国病毒病杂志,2017,7(2):113-119.
[2] Li L,He Y,Yang H,et al.Genetic characteristics of human enterovirus 71 and coxsackievirus A16 People’s Republic of China[J].Clin Microbiol,2005,43(8):3835-3839.
[3] 任倩,马丽霞,张乐海.肠道病毒71致病机制的研究进展[J].国际流行病学传染病学杂志,2011,38(3):197-200.
[4] 王国玲,谭锦花,刘青连,等.广州市海珠区2008- 2012 年手足口病流行特征及病原学分析[J].中国卫生检验杂志,2014,24(5):688- 690.
[5] Yang F,Ren LL,Xiong ZH,et al.Enterovirus 71 outbreak inthe people’s republic of China in 2008[J].J Clin Microbiol,2009,47(7):2351-2352.
[6] 牛文柯,丁淑军,林艺,等.山东省2009-2010年手足口病死亡病例流行病学及临床特征分析[J].中国儿童保健杂志,2012,20(3):263-266.
[7] 白丽娜,王冰,王萍,等.2015年沈阳地区手足口病监测结果分析[J].中国卫生检验杂志,2016,26(20):2980-2981.
[8] 王永全,吉彦莉,曲梅.北京地区与手足口病相关的非EV714,非CA16 型肠道病毒的分子特征分析[J].国际病原学杂志,2011,18(6):75-79.
[9] 刘雅琼,袁伟,吕强.2009-2016年四川省手足口病流行病学和病原学特征分析[J].中华疾病控制杂志,2017,21(10):1052-1056.
[10] 王芬,方涛,祝绯飞,等.2010-2014年杭州市拱墅区手足口病疫情流行特征[J].疾病监测,2015,30(9):732-735.
[11] 李晓军,李芳,詹海丰.上海市普陀区2013 年手足口病病原学监测结果分析[J].海南医学,2015,26(5):755-757.
[12] 陈纯,肖新才,谢华萍,等.2008 -2013年广州市手足口病流行病学特征研究[J].疾病监测,2014,29(12):964-968.
[13] 王侃.锦州市2014-2016 年手足口病病原学研究[J].疾病监测与控制杂志,2017,11 (7):526-527.
[14] 丁克琴,陈奕,谢蕾,等.2011-2015 年宁波市手足口病流行病学与病原学特征分析[J].现代预防医学,2016,43(23):4251-4263.
[15] 何为涛,蒋丽娜,王东燕,等.广西2008-2015年手足口病流行病学特征及病原学监测分析[J].中国病毒病杂志,2017,7(2):113-119.