2017年河北长爪沙鼠疫源地鼠疫菌的生态及差异区段分型研究
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摘要
目的研究2017年河北康保长爪沙鼠疫源地鼠疫耶尔森菌的生态及差异片段(DFR)分型,探讨其流行特征。方法分离培养鼠疫菌,进行糖醇酵解实验以确定生态型;煮沸法提取鼠疫菌DNA,进行23对DFR和PMT1质粒序列PCR扩增,并进行PCR产物电泳分析。结果 3株菌(201701、201702和201703)均发酵甘油,阿胶糖产酸不产气;不发酵麦芽糖,鼠李糖;201701,201702两株菌迟缓发酵蜜二糖,201703不发酵蜜二糖;脱氮均为阴性。201701、201702、201703菌株均缺失DFR位点1、6、7、12、13、15、16、17、18、23。结论 2017年河北省鼠疫菌株生态型为B群鄂尔多斯高原型,差异片段分型为G20型。
Objective To study the ecological classification and different regional genotype(DFR) of Yersinia pestis in Mongolian gerbil epidemic focus in Kangbao County of Hebei Province in 2017,and to know its epidemic features.Methods Yersinia pestis was isolated and cultured.Glycolysis experiment was conducted to determine its ecological type,and boiling method was used to isolate its DNA for DFR and PMT1 plasmid sequence amplification.The PCR products were analyzed with electrophoretic assay.Results Three strains of Yersinia pestis could ferment glycerin and gelatin candy,and produce acid but not gas.They did not ferment maltose and rhamnose.Among them,strains 201701 and 201702 ferment melibiose slowly,while strain 201703 did not ferment melibiose at all.Their denitrification results were all negative.DFR loci 1,6,7,12,13,15,16,17,18,and 23 were missing in strain 201701,201702 and 201703.Conclusion The ecological type of Yersinia pestis strains in Hebei 2017 was group B of Ordos plateau type,and the regional genotype was G20.
Objective To study the ecological classification and different regional genotype(DFR) of Yersinia pestis in Mongolian gerbil epidemic focus in Kangbao County of Hebei Province in 2017,and to know its epidemic features.Methods Yersinia pestis was isolated and cultured.Glycolysis experiment was conducted to determine its ecological type,and boiling method was used to isolate its DNA for DFR and PMT1 plasmid sequence amplification.The PCR products were analyzed with electrophoretic assay.Results Three strains of Yersinia pestis could ferment glycerin and gelatin candy,and produce acid but not gas.They did not ferment maltose and rhamnose.Among them,strains 201701 and 201702 ferment melibiose slowly,while strain 201703 did not ferment melibiose at all.Their denitrification results were all negative.DFR loci 1,6,7,12,13,15,16,17,18,and 23 were missing in strain 201701,201702 and 201703.Conclusion The ecological type of Yersinia pestis strains in Hebei 2017 was group B of Ordos plateau type,and the regional genotype was G20.
引文
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[11] 王海峰,刘合智,白雪薇,等.河北省116株鼠疫耶尔森菌差异片段基因分型及流行病学分析[J].中国媒介生物学及控制杂志,2016,27(5):470-473.
[12] 岳珊珑,戴二黑,周冬生,等.44株中国田鼠型与24株非田鼠型鼠疫菌的DFR检测分析[J].中华微生物学和免疫学杂志,2005,25(6):490-493.
[13] 戴二黑,童宗中,王孝义,等.应用抑制消减杂交技术进行鼠疫耶尔森菌的比较基因组研究[J].中华微生物学和免疫学杂志,2005,25(3):179-182.
[2] 周冬生,韩延平,宋亚军,等.鼠疫耶尔森氏菌基因组进化与生态位适应研究[J].解放军医学杂志,2004,29(3):204-210.
[3] 夏连续,海荣.鼠疫实验室技术手册[M].北京:北京出版社,2013:220-223.
[4] 纪树立,张海凌,刘云鹏,等.我国鼠疫菌分型及其生态学,流行病学意义[J].中国地方病学杂志,1987,6(5):257-263.
[5] 杨晓艳,魏柏青,靳娟,等.中国鼠疫耶尔森菌差异区段分型及其地理分布特征[J].中华流行病学杂志,2014,35(8):943-948.
[6] 刘合智,张月芝,史献明,等.河北省鼠疫菌株生化特性的研究[J].中国媒介生物学及控制杂志,2005,16(4):308-310.
[7] 周冬生,杨瑞馥.细菌基因组进化的分子策略[J].微生物学杂志,2004,24(1):34-41.
[8] Zhou DS,Han YP,Song YJ.DNA microarray analysis of genome dynamics in Yersinia pestis insight into bacterial genome microevolution and niche adaptation[J].PLA Med J,2004,29(3):204-210.
[9] 周冬生,韩延平,宋亚军,等.鼠疫耶尔森菌基因组进化与生态位适应研究[J].解放军医学杂志,2004,29(3):204-210.
[10] 杨瑞馥,黄培堂.鼠疫耶尔森菌比较和进化基因组学研究[J].解放军医学杂志,2004,29(3):189-191.
[11] 王海峰,刘合智,白雪薇,等.河北省116株鼠疫耶尔森菌差异片段基因分型及流行病学分析[J].中国媒介生物学及控制杂志,2016,27(5):470-473.
[12] 岳珊珑,戴二黑,周冬生,等.44株中国田鼠型与24株非田鼠型鼠疫菌的DFR检测分析[J].中华微生物学和免疫学杂志,2005,25(6):490-493.
[13] 戴二黑,童宗中,王孝义,等.应用抑制消减杂交技术进行鼠疫耶尔森菌的比较基因组研究[J].中华微生物学和免疫学杂志,2005,25(3):179-182.