Ku80在甲状腺乳头状癌组织中的表达及其临床意义
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摘要
目的:探讨甲状腺乳头状癌组织中Ku80的表达水平及其与临床病理特征的关系。方法:收集22例甲状腺乳头状癌患者的组织蜡块,应用免疫组化检测甲状腺乳头状癌、癌旁组织中Ku80的表达,并分析Ku80表达与甲状腺乳头状癌临床病理特征的关系。结果:甲状腺乳头状癌组织中Ku80阳性表达率为63. 6%,癌旁组织阳性表达率为22. 7%,差异有统计学意义(P <0. 05); Ku80表达与患者的肿瘤TNM分期中T分期有关(P=0. 005),与患者年龄、性别、是否有淋巴结转移、肿瘤大小、发病部位及是否有其他合并症无显著相关性(P> 0. 05)。结论:Ku80在甲状腺乳头状癌中高表达,并与肿瘤T分期显著相关。
Objective: To investigate the expression of Ku80 in papillary thyroid carcinoma( PTC) and its relationship with clinicopathological features. Methods: Twenty-two paraffin blocks of thyroid papillary carcinoma were collected and the expression of Ku80 in papillary thyroid carcinoma and paracancerous tissues were detected by immunohistochemistry. The relationship between Ku80 expression and clinicopathological features of papillary thyroid carcinoma was analyzed. Results: The positive expression rate of Ku80 in thyroid papillary carcinoma tissue was 63. 6%,while that in paracancerous tissue was 22. 7%( P < 0. 05). The expression of Ku80 was correlated with T stage of patients( P = 0. 005),while it did not related to patient's age,gender,lymph node metastasis,tumor size,location of the tumor,and other complications( P > 0. 05). Conclusion: Ku80 is highly expressed in papillary thyroid carcinoma and is significantly related to the T stage of the patient with papillary thyroid carcinoma.
Objective: To investigate the expression of Ku80 in papillary thyroid carcinoma( PTC) and its relationship with clinicopathological features. Methods: Twenty-two paraffin blocks of thyroid papillary carcinoma were collected and the expression of Ku80 in papillary thyroid carcinoma and paracancerous tissues were detected by immunohistochemistry. The relationship between Ku80 expression and clinicopathological features of papillary thyroid carcinoma was analyzed. Results: The positive expression rate of Ku80 in thyroid papillary carcinoma tissue was 63. 6%,while that in paracancerous tissue was 22. 7%( P < 0. 05). The expression of Ku80 was correlated with T stage of patients( P = 0. 005),while it did not related to patient's age,gender,lymph node metastasis,tumor size,location of the tumor,and other complications( P > 0. 05). Conclusion: Ku80 is highly expressed in papillary thyroid carcinoma and is significantly related to the T stage of the patient with papillary thyroid carcinoma.
引文
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[14]Yan Shanshan,Liu Li,Liu Zhigang,et al.Expression of Ku80 in nasopharyngeal carcinoma and its clinical significance[J].Cancer Prevention and Treatment,2008,21(1):49-52.[严珊珊,刘莉,刘志刚,等.Ku80在鼻咽癌中的表达及其临床意义[J].肿瘤预防与治疗,2008,21(1):49-52.]
[15]Tuteja R,Tuteja N.Ku Autoantigen:A multifunctional DNA-binding protein[J].Crc Critical Reviews in Biochemistry,2000,35(1):1-33.
[16]Lim JW,Kim H,Kim KH.Expression of Ku70 and Ku80 mediated by NF-kappa B and cyclooxygenase-2 is related to proliferation of human gastric cancer cells[J].J Biol Chem,2002,277(48):46093-46100.
[17]Li G,Nelsen C,Hendrickson EA.Ku86 is essential in human somatic cells[J].Proc Natl Acad Sci USA,2002,99(2):832-837.
[2]Semenciw R.Increasing thyroid cancer incidence in Canada,1970[ndash]1996:Time trends and age-period-cohort effects[J].British Journal of Cancer,2001,85(9):1335.
[3]Haugen BR,Alexander EK,Bible KC,et al.2015 American thyroid association management guidelines for adult patients with thyroid nodules and differentiated thyroid cancer:The american thyroid association guidelines task force on thyroid nodules and differentiated thyroid cancer[J].Thyroid,2016,26(1):1.
[4]Lieber MR,Ma Y,Pannicke U,et al.The mechanism of vertebrate nonhomologous DNA end joining and its role in V(D)J recombination[J].DNA Repair,2004,3(8-9):817-826.
[5]Schild-Poulter C,HachéRJG,Soubeyrand S,et al.Ku antigen:Aversatile DNA binding protein with multiple cellular functions[J].Dynamical Genetics,Research Signpost,2004,4(15):257-284.
[6]Liu RY,Zeng Y,Lei Z,et al.JAK/STAT3 signaling is required for TGF-β-induced epithelial-mesenchymal transition in lung cancer cells[J].International Journal of Oncology,2014,44(5):1643-1651.
[7]Kleihues P,Stewart BW.World cancer report[J].Lancet Oncology,2003,4(5):321.
[8]Williams D.Radiation carcinogenesis:Lessons from Chernobyl[J].Oncogene,2008,27(2):9-18.
[9]Dobbs TA,Tainer JA,Lees-Miller ASP.A structural model for regulation of NHEJ by DNA-PKcs autophosphorylation[J].DNARepair,2010,9(12):1307.
[10]Gullo C,Au M,Feng G,et al.The biology of Ku and its potential oncogenic role in cancer[J].Biochim Biophys Acta,2006,1765(2):223-234.
[11]Qu Song,Liang Shixiong,Zhu Xiaodong.A research about the relationship between Ku protein and DNA-PK and tumor therapy sensitivity[J].Modern Oncology,2006,14(1):114-116.[曲颂,梁世雄,朱小东.Ku蛋白及DNA-PK与肿瘤治疗敏感性关系的研究进展[J].现代肿瘤医学,2006,14(1):114-116.]
[12]Lowe SW,Schmitt EM,Smith SW,et al.p53 is required for radiation-induced apoptosis in mouse thymocytes[J].Nature,1993,362(6423):847-849.
[13]Ribeiro JC,Barnetson AR,Fisher RJ,et al.Relationship between radiation response and p53 status in human bladder cancer cells[J].International Journal of Radiation Biology,1997,72(1):11-20.
[14]Yan Shanshan,Liu Li,Liu Zhigang,et al.Expression of Ku80 in nasopharyngeal carcinoma and its clinical significance[J].Cancer Prevention and Treatment,2008,21(1):49-52.[严珊珊,刘莉,刘志刚,等.Ku80在鼻咽癌中的表达及其临床意义[J].肿瘤预防与治疗,2008,21(1):49-52.]
[15]Tuteja R,Tuteja N.Ku Autoantigen:A multifunctional DNA-binding protein[J].Crc Critical Reviews in Biochemistry,2000,35(1):1-33.
[16]Lim JW,Kim H,Kim KH.Expression of Ku70 and Ku80 mediated by NF-kappa B and cyclooxygenase-2 is related to proliferation of human gastric cancer cells[J].J Biol Chem,2002,277(48):46093-46100.
[17]Li G,Nelsen C,Hendrickson EA.Ku86 is essential in human somatic cells[J].Proc Natl Acad Sci USA,2002,99(2):832-837.