三氯生对雄性斑马鱼鳃组织凋亡相关基因表达的影响
|
摘要
为探究三氯生(TCS)对于硬骨鱼类的凋亡相关基因表达的影响,以雄性斑马鱼(Danio Rerio)为试验动物,采用半静态水体接触染毒法,将斑马鱼分别置于质量浓度为17、34和68μg/L的TCS溶液中,并设计空白对照组,42 d后,对鳃组织进行采样,利用普通PCR和RT-qPCR技术分析雄性斑马鱼鳃组织凋亡基因Bcl-2、Bax、MDM2、p53的表达量。研究发现,与空白对照组相比,Bax和p53基因在TCS处理组中的表达量都出现了下调趋势,且在68μg/L的TCS处理组中表达水平显著下调;Bcl-2在各TCS处理组中无显著变化;MDM2基因在34μg/L和68μg/L TCS处理浓度组中表现为极显著上调。研究结果表明:高浓度TCS对雄性斑马鱼鳃组织凋亡相关基因的表达具有一定的影响。
Danio rerio was chosen as experimental subject to investigate the effection of triclosan(TCS) on the expression of apoptosis-related genes in bony fishes. D. rerio were exposed to 0(control), 17, 34, and 68 μg/L TCS respectively using the method of semi static water contact staining. The expressions of apoptosis-related genes in gill tissue of male zebrafish were explored by using general Polymerase Chain Reaction(PCR) and Real-Time Quantitative PCR(RT-qPCR) technique.The results showed that the expressions of Bax and p53 gene in TCS treated groups put up a downward trend compared with the control group, which were significantly decreased in 68 μg/L TCS treated group. Bcl-2 did not change significantly in TCS treated group, MDM2 gene was significantly increased in 34 μg/L and 68 μg/L TCS concentration groups. The results showed that high-concentration TCS had a significant effection on the expression of apoptosis-related genes in the gill of male zebrafish.
Danio rerio was chosen as experimental subject to investigate the effection of triclosan(TCS) on the expression of apoptosis-related genes in bony fishes. D. rerio were exposed to 0(control), 17, 34, and 68 μg/L TCS respectively using the method of semi static water contact staining. The expressions of apoptosis-related genes in gill tissue of male zebrafish were explored by using general Polymerase Chain Reaction(PCR) and Real-Time Quantitative PCR(RT-qPCR) technique.The results showed that the expressions of Bax and p53 gene in TCS treated groups put up a downward trend compared with the control group, which were significantly decreased in 68 μg/L TCS treated group. Bcl-2 did not change significantly in TCS treated group, MDM2 gene was significantly increased in 34 μg/L and 68 μg/L TCS concentration groups. The results showed that high-concentration TCS had a significant effection on the expression of apoptosis-related genes in the gill of male zebrafish.
引文
[1]Reiss R,Mackay N,Habig C,et al.An ecological risk assessment for triclosan in lotic systems following discharge from wastewater treatment plants in the United States [J].Environ Toxicol Chem,2002,21(11):2483-2492.
[2]Corteza F S,Seabra Pereira C D,Santos A R,et al.Biological effects of environmentally relevant concentra-tions of the pharmaceμtical triclosan in the marine mμs-sel Perna perna(Linnaeμs) [J].Environ Pollμtion,2012,168:145-150.
[3]郑欣,刘婷婷,王一喆,等.三氯生毒性效应及水质基准研究进展[J].生态环境学报,2016,25(3):539-546.
[4]刘飞,刘旭昊,王凡,等.三氯生对红白鲫的急性毒性及遗传毒性研究[J].水生态学杂志,2016,37(5):82-86.
[5]危玲,吴青青.三氯生的生殖毒性[J].中国计划生育学杂志,2015,23(11):783-785.
[6]陈建军,豆晓飞,刘海津,等.三氯生致泥鳅生理及遗传毒性作用[J].中国公共卫生,2013,29(7):1014-1016.
[7] AliceHontela,Hamid R.Habibi.Personal care products in the aquatic environment:a case study on the effects of triclosan in fish [J].Fish Physiol,2013,33(5):67-69.
[8] Zhou B,Liu C,Wang J,et al.Primary cultured cells as sensitive in vitro model for assessment of toxicants-comparison to hepatocytes and gill epithelia [J].Aquat Toxicol,2006,80(2):109-118.
[9] Oliveira R,Domingues I,Grisolia C K,et al.Effects of triclosan on zebrafish early-life stages and adults [J].Environ Sci Pollution Res,2009,16(6):679-688.
[10] Wu S M,Liu J H,Shu L H,et al.Anti-oxidative responses of zebrafish(Danio rerio) gill,liver and brain tissues upon acute cold shock [J].Comp Biochem Physiol Part A:Mol Integr Physiol,2015,187:202-213.
[11]付永锋,樊廷俊.Bcl-2家族蛋白与细胞凋亡[J].生物化学与生物物理学报,2002,34(4):389-394.
[12]Huang Q,Bu S,Yu Y,et al.Diazoxide prevents diabetes throμgh inhibiting pancreatic beta-cells from apoptosis via Bcl-2/Bax rate and p38-beta mitogen-activated protein kinase [J].Endocrinology,2007,148(1):81-91.
[13]赵燕,杨秋萍,肖华,等.灯盏花素对糖尿病大鼠肾脏细胞凋亡及bax、bcl-2表达的影响[J].中国糖尿病杂志,2009,17(11):864-867.
[14]Cobellis L,Defalco M,Torella M,et al.Modulation of Bax expression in physiological and pathological human placentas throughout pregnancy [J].In Vivo,2007,21(5):777-783.
[15]张德芹,张建军,钟赣生,等.芪蓝糖脂宁胶囊对糖尿病合并高脂血症大鼠肝细胞凋亡及Bax、Bcl-2蛋白表达的影响[J].中华中医药杂志,2005,20(4):211-213.
[16] Emi M,Kim R,Tanabe K,et al.Targeted therapy against Bcl-2-related proteins in breast cancer cells [J].Breast Cancer Res,2005,7(6):940-952.
[17]刘林,赵群芬,金凯星,等.纳米氧化锌对斑马鱼肝脏的毒性效应[J].环境科学,2015,36(10):3884-3891.
[18]鲁疆,王占洋,袁玉婷,等.氯化镉对斑马鱼胚胎的发育毒性[J].生态毒理报,2013,8(3):381-388.
[19]魏永永,侯静,唐文如,等.p53与Ras协同及其在肿瘤发生中的作用[J].遗传,2012,34(12):1513-1521.
[20]Livesey K M,Kang R,Vernon P,et al.p53/HMGB1 complexes regulate autophagy and apoptosis [J].Autophagy,2012,72(5):1996-2005.
[21] Dong XX,Wang Y R,Qin S,et al.p53 mediates autophagy activation and mitochondria dysfunction in kainic acid-induced excitotoxicity in primary striatal neurons [J].Neuroscience,2012,207:52-64.
[22] Shen L,Sun X,Fu Z,et al.The Fundamental Role of the p53 Pathway in Tumor Metabolism and Its Implication in Tumor Therapy [J].Clin Cancer Res An Off J Am Assoc Cancer Res,2012,18(6):1561-1567.
[23]林春兰,付步银,周羽竝,等.头颈部鳞癌中p53-mdm2基因异常的研究[J].暨南大学学报(自然科学与医学版),2006,27(4):573-576.
[2]Corteza F S,Seabra Pereira C D,Santos A R,et al.Biological effects of environmentally relevant concentra-tions of the pharmaceμtical triclosan in the marine mμs-sel Perna perna(Linnaeμs) [J].Environ Pollμtion,2012,168:145-150.
[3]郑欣,刘婷婷,王一喆,等.三氯生毒性效应及水质基准研究进展[J].生态环境学报,2016,25(3):539-546.
[4]刘飞,刘旭昊,王凡,等.三氯生对红白鲫的急性毒性及遗传毒性研究[J].水生态学杂志,2016,37(5):82-86.
[5]危玲,吴青青.三氯生的生殖毒性[J].中国计划生育学杂志,2015,23(11):783-785.
[6]陈建军,豆晓飞,刘海津,等.三氯生致泥鳅生理及遗传毒性作用[J].中国公共卫生,2013,29(7):1014-1016.
[7] AliceHontela,Hamid R.Habibi.Personal care products in the aquatic environment:a case study on the effects of triclosan in fish [J].Fish Physiol,2013,33(5):67-69.
[8] Zhou B,Liu C,Wang J,et al.Primary cultured cells as sensitive in vitro model for assessment of toxicants-comparison to hepatocytes and gill epithelia [J].Aquat Toxicol,2006,80(2):109-118.
[9] Oliveira R,Domingues I,Grisolia C K,et al.Effects of triclosan on zebrafish early-life stages and adults [J].Environ Sci Pollution Res,2009,16(6):679-688.
[10] Wu S M,Liu J H,Shu L H,et al.Anti-oxidative responses of zebrafish(Danio rerio) gill,liver and brain tissues upon acute cold shock [J].Comp Biochem Physiol Part A:Mol Integr Physiol,2015,187:202-213.
[11]付永锋,樊廷俊.Bcl-2家族蛋白与细胞凋亡[J].生物化学与生物物理学报,2002,34(4):389-394.
[12]Huang Q,Bu S,Yu Y,et al.Diazoxide prevents diabetes throμgh inhibiting pancreatic beta-cells from apoptosis via Bcl-2/Bax rate and p38-beta mitogen-activated protein kinase [J].Endocrinology,2007,148(1):81-91.
[13]赵燕,杨秋萍,肖华,等.灯盏花素对糖尿病大鼠肾脏细胞凋亡及bax、bcl-2表达的影响[J].中国糖尿病杂志,2009,17(11):864-867.
[14]Cobellis L,Defalco M,Torella M,et al.Modulation of Bax expression in physiological and pathological human placentas throughout pregnancy [J].In Vivo,2007,21(5):777-783.
[15]张德芹,张建军,钟赣生,等.芪蓝糖脂宁胶囊对糖尿病合并高脂血症大鼠肝细胞凋亡及Bax、Bcl-2蛋白表达的影响[J].中华中医药杂志,2005,20(4):211-213.
[16] Emi M,Kim R,Tanabe K,et al.Targeted therapy against Bcl-2-related proteins in breast cancer cells [J].Breast Cancer Res,2005,7(6):940-952.
[17]刘林,赵群芬,金凯星,等.纳米氧化锌对斑马鱼肝脏的毒性效应[J].环境科学,2015,36(10):3884-3891.
[18]鲁疆,王占洋,袁玉婷,等.氯化镉对斑马鱼胚胎的发育毒性[J].生态毒理报,2013,8(3):381-388.
[19]魏永永,侯静,唐文如,等.p53与Ras协同及其在肿瘤发生中的作用[J].遗传,2012,34(12):1513-1521.
[20]Livesey K M,Kang R,Vernon P,et al.p53/HMGB1 complexes regulate autophagy and apoptosis [J].Autophagy,2012,72(5):1996-2005.
[21] Dong XX,Wang Y R,Qin S,et al.p53 mediates autophagy activation and mitochondria dysfunction in kainic acid-induced excitotoxicity in primary striatal neurons [J].Neuroscience,2012,207:52-64.
[22] Shen L,Sun X,Fu Z,et al.The Fundamental Role of the p53 Pathway in Tumor Metabolism and Its Implication in Tumor Therapy [J].Clin Cancer Res An Off J Am Assoc Cancer Res,2012,18(6):1561-1567.
[23]林春兰,付步银,周羽竝,等.头颈部鳞癌中p53-mdm2基因异常的研究[J].暨南大学学报(自然科学与医学版),2006,27(4):573-576.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |