京科968花丝抗螟性评价及其抗性机制研究
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摘要
玉米品种京科968具有众多综合优良特性,是北方春玉米区的主栽品种。为了解京科968穗期对亚洲玉米螟的抗性水平,并探明其抗螟机制,本试验选取京科968,其母本京724和父本京92,以及自330等4个玉米品种(系)作为供试玉米材料,其中自330作为感螟对照。通过实验种群生命表技术对取食4个玉米品种(系)花丝的亚洲玉米螟种群的生命表参数进行评价,并对花丝中营养物质、防御酶以及丁布类等化合物的含量进行分析。生命表结果显示,4个玉米品种(系)抗螟性大小依次为:京92>京科968>京724>自330。与京724和感虫对照自330相比,取食京科968花丝的亚洲玉米螟发育历期延长,平均世代周期长,说明京科968的花丝具有一定抗螟效果;生化分析结果显示京科968花丝中的具有较高的可溶性糖、可溶性蛋白质、多酚氧化酶和超氧化物歧化酶含量,而DIMBOA和DIMBOA-Glc的含量均较低,说明玉米防御酶可能在花丝抗螟中发挥重要作用。
Corn hybrid Jingke 968, is one of the main planted corn varieties in north China with good comprehensive characteristic. In order to understand the resistance of Jingke 968 and its resistant mechanism to Asian corn borer(ACB), Ostrinia furnacalis, in silking stage, Jingke 968, its female parent Jing 724 and male parent Jing 92 were selected in the experiment, inbred line Zi 330, susceptible to ACB, was as the control. The parameters of the ACB population fed on silk of the four tested corn hybrids(inbreds) were evaluated by life table technique, the content of nutrient, defense enzyme and DIMBOA compounds in silk was analyzed. Results showed that the resistance level of corn silk to ACB of the tested corn hybrid or inbreds were arranged as follows from resistant to susceptible: Jing 92> Jingke 968> Jing 724>Zi 330. And the growth and development time of ACB population fed on Jingke 968 silk was prolonged and the mean generation time was longer compared with Jing 724 and Zi 330. The silk of Jingke 968 was resistant to ACB to some extent. Biochemical analysis showed that the silk of Jingke 968 had high content of soluble sugar and protein, PPO and SOD, and the content of DIMBOA compounds were low, indicating that the defense enzymes in silk of Jingke 968 may play an important role in silk resistance to ACB.
Corn hybrid Jingke 968, is one of the main planted corn varieties in north China with good comprehensive characteristic. In order to understand the resistance of Jingke 968 and its resistant mechanism to Asian corn borer(ACB), Ostrinia furnacalis, in silking stage, Jingke 968, its female parent Jing 724 and male parent Jing 92 were selected in the experiment, inbred line Zi 330, susceptible to ACB, was as the control. The parameters of the ACB population fed on silk of the four tested corn hybrids(inbreds) were evaluated by life table technique, the content of nutrient, defense enzyme and DIMBOA compounds in silk was analyzed. Results showed that the resistance level of corn silk to ACB of the tested corn hybrid or inbreds were arranged as follows from resistant to susceptible: Jing 92> Jingke 968> Jing 724>Zi 330. And the growth and development time of ACB population fed on Jingke 968 silk was prolonged and the mean generation time was longer compared with Jing 724 and Zi 330. The silk of Jingke 968 was resistant to ACB to some extent. Biochemical analysis showed that the silk of Jingke 968 had high content of soluble sugar and protein, PPO and SOD, and the content of DIMBOA compounds were low, indicating that the defense enzymes in silk of Jingke 968 may play an important role in silk resistance to ACB.
引文
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Niemeyer HM. Hydroxamic acids derived from 2-Hydroxy-2H-1,4-Benzoxazin-3(4H)-one: key defense chemicals of cereals[J].J. Agr. Food Chem., 2009, 57(5): 1677-96.
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Szalma SJ, Buckler ES, Snook ME, et al. Association analysis of candidate genes for maysin and chlorogenic acid accumulation in maize silks[J]. Theor. Appl. Genet., 2005, 110(7): 1324-1333.
Wang CZ, Zhang QW, Yang QH,et al. The chemistry basis of insect resistance in plant[J]. Plant Protection, 1993, 19(6): 39-41. [王琛柱, 张青文, 杨奇华, 等. 植物抗虫性的化学基础[J]. 植物保护, 1993, 19(6): 39-41]
Wang YS, Ren GM. The relationship between corn sugar content and density and infestation of Ostrinia furnacalis[J]. Plant Protection, 1982, 8(6): 5. [汪郁生, 任光明. 玉米含糖量与玉米螟密度及为害的关系[J]. 植物保护, 1982, 8(6): 5]
Wang ZY, Lu X, He KL, et al. Review of history, present situation and prospect of the Asian maize borer research in China[J]. Journal of Shenyang Agricultural University, 2000, 31(5): 402-412. [王振营, 鲁新, 何康来, 等. 我国研究亚洲玉米螟历史、现状与展望[J]. 沈阳农业大学学报, 2000, 31(5): 402-412]
Wang ZY. The biochemistry basis of insect resistance in maize[J].World Agriculture, 1994, 8: 33-34. [王振营. 玉米抗虫的生化基础[J]. 世界农业, 1994, 8: 33-34]
Wu KJ, Chen YP, Li MH. Life table of laboratory population of Helicoverpa armigera (Hübner) at different temperatures[J]. Acta Entomologica Sinica, 1978, 21(4): 385-392. [吴坤君, 陈玉平, 李明辉. 不同温度下的棉铃虫实验种群生命表[J]. 昆虫学报, 1978, 21(4): 385-392]
Wu YB, Tian FJ, Zhao HH, et al. Research progress on the biological activity of DIMBOA[J]. Plant Protection, 2014, 40(5): 8-13.[吴艳兵, 田发军, 赵欢欢, 等. 丁布的生物活性研究进展[J]. 植物保护, 2014, 40(5): 8-13]
Zhao M, Guo XR, Li WZ,et al. Effects of different maize hybrids (inbreds) on the growth, development and population dynamics of Rhopalosiphum maidis Fitch[J]. Acta Ecologica Sinica, 2013, 33(15): 4707-4714.[赵曼, 郭线茹, 李为争, 等. 不同玉米品种(系)对玉米蚜生长发育和种群增长的影响[J]. 生态学报, 2013, 33(15): 4707-4714]
Zhou DR, Wang YY, Liu BL,et al. The research of mass rearing of corn borer, Ⅰ. The semi-artificial diet and its improvement[J]. Acta Phytophylacica Sinica, 1980, 7(2): 113-122. [周大荣, 王玉英, 刘宝兰, 等. 玉米螟人工大量繁殖研究:Ⅰ.一种半人工饲料及其改进[J]. 植物保护学报, 1980, 7(2): 113-122]
Ana Butrón, Chen YC, Rottinghaus GE, et al. Genetic variation at bx1 controls DIMBOA content in maize[J]. Theor. Appl. Genet., 2010, 120(4): 721-734.
Chen MS. Inducible direct plant defense against insect herbivores: A review[J]. Insect Sci., 2010,15(2):101-114.
Cheng LX, He SQ, Yan NS,et al. Study on the pupae weight of the geographical and laboratory population under different food and temperature of Asian corn borer, Ostrinia furnacalis Guenée (Lepidoptera: Pyralidae)[J]. Journal of Environmental Entomology, 2014, 36(3): 322-326. [程鲁雪, 和淑琪, 严乃胜, 等. 亚洲玉米螟地理种群不同食物及温度下实验种群的蛹重研究[J]. 环境昆虫学报, 2014, 36(3): 322-326]
Dhillon M K, Kumar S. Amino acid profiling of Sorghum bicolor vis-à-vis Chilo partellus (Swinhoe) for biochemical interactions and plant resistance[J]. Arthropod-Plant Inte., 2017, 11(4): 537-550.
Glauser G, Marti G, Villard N,et al. Induction and detoxification of maize 1,4-benzoxazin-3-ones by insect herbivores[J]. Plant J., 2011, 68(5): 901-911.
Guo JF, Qi JF, He KL,et al. The Asian corn borer Ostrinia furnacalis feeding increases the direct and indirect defense of mid-whorl stage commercial maize in the field[J]. Plant Biotechnol. J., 2018.
He KL, Wang ZY, Zhou DR,et al. Methodologies and criterions for evaluating maize resistance to Asian maize borer[J]. Journal of Shenyang Agricultural University, 2000, 31(5): 439-443. [何康来, 王振营, 周大荣, 等. 玉米抗螟性鉴定方法与评价标准[J]. 沈阳农业大学学报, 2000, 31(5): 439-443.]
Kojima W, Fujii T, Suwa M,et al. Physiological adaptation of the Asian corn borer Ostrinia furnacalis to chemical defenses of its host plant, maize[J]. J. Insect Physiol., 2010, 56(9): 1349-1355.
Li X, He KL, Wang ZY,et al. Quantitative trait loci for Asian corn borer resistance in maize population Mc37×Zi330[J]. J. Integr. Agr., 2010, 9(1): 77-84.
Li XZ, Liu YH, Tian Y. Effects of six host plants on the development and fecundity of Cicadulina bipunctella[J].Chinese Journal of Applied Ecology, 2004, 15(8): 1431-1434. [李小珍, 刘映红, 田艳. 六种寄主植物对二点叶蝉生长发育和繁殖的影响[J]. 应用生态学报, 2004, 15(8):1431-1434]
Luo MH, Zhao YY, Liu XG,et al. Studied on insect-resistance of maize with different genotypes[J]. Journal of Maize Sciences, 2007, 15(5): 34-37. [罗梅浩, 赵艳艳, 刘哓光, 等. 不同玉米品种的抗虫性研究[J]. 玉米科学, 2007, 15(5): 34-37]
Niemeyer HM. Hydroxamic acids derived from 2-Hydroxy-2H-1,4-Benzoxazin-3(4H)-one: key defense chemicals of cereals[J].J. Agr. Food Chem., 2009, 57(5): 1677-96.
Qin QJ, Gao XW. Plant defense responses induced by insect herbivory[J]. Acta Entomogica Sinica, 2005, 48(1): 125-134. [秦秋菊, 高希武. 昆虫取食诱导的植物防御反应[J]. 昆虫学报, 2005, 48(1): 125-134.]
Guo JF, Qi JF, He KL, et al. The Asian corn borer Ostrinia furnacalis feeding increases the direct and indirect defense of mid-whorl stage commercial maize in the field[J]. Plant Biotechnology Journal, 2018, 1-15. doi: 10.1111/pbi.12949.
Scott LM, Thaler JS, Scott JG. Response of a generalist herbivore Trichoplusia ni to jasmonate-Mediated induced defense in tomato[J]. J. Chem. Ecol., 2010, 36(5): 490-499.
Storer NP, van Duyn JW, Kennedy GG. Life history traits of Helicoverpa zea (Lepidoptera: Noctuidae) on non-Bt and Bt transgenic corn hybrids in eastern North Carolina[J]. J. Econ. Entomol., 2001, 94(5): 1268.
Szalma SJ, Buckler ES, Snook ME, et al. Association analysis of candidate genes for maysin and chlorogenic acid accumulation in maize silks[J]. Theor. Appl. Genet., 2005, 110(7): 1324-1333.
Wang CZ, Zhang QW, Yang QH,et al. The chemistry basis of insect resistance in plant[J]. Plant Protection, 1993, 19(6): 39-41. [王琛柱, 张青文, 杨奇华, 等. 植物抗虫性的化学基础[J]. 植物保护, 1993, 19(6): 39-41]
Wang YS, Ren GM. The relationship between corn sugar content and density and infestation of Ostrinia furnacalis[J]. Plant Protection, 1982, 8(6): 5. [汪郁生, 任光明. 玉米含糖量与玉米螟密度及为害的关系[J]. 植物保护, 1982, 8(6): 5]
Wang ZY, Lu X, He KL, et al. Review of history, present situation and prospect of the Asian maize borer research in China[J]. Journal of Shenyang Agricultural University, 2000, 31(5): 402-412. [王振营, 鲁新, 何康来, 等. 我国研究亚洲玉米螟历史、现状与展望[J]. 沈阳农业大学学报, 2000, 31(5): 402-412]
Wang ZY. The biochemistry basis of insect resistance in maize[J].World Agriculture, 1994, 8: 33-34. [王振营. 玉米抗虫的生化基础[J]. 世界农业, 1994, 8: 33-34]
Wu KJ, Chen YP, Li MH. Life table of laboratory population of Helicoverpa armigera (Hübner) at different temperatures[J]. Acta Entomologica Sinica, 1978, 21(4): 385-392. [吴坤君, 陈玉平, 李明辉. 不同温度下的棉铃虫实验种群生命表[J]. 昆虫学报, 1978, 21(4): 385-392]
Wu YB, Tian FJ, Zhao HH, et al. Research progress on the biological activity of DIMBOA[J]. Plant Protection, 2014, 40(5): 8-13.[吴艳兵, 田发军, 赵欢欢, 等. 丁布的生物活性研究进展[J]. 植物保护, 2014, 40(5): 8-13]
Zhao M, Guo XR, Li WZ,et al. Effects of different maize hybrids (inbreds) on the growth, development and population dynamics of Rhopalosiphum maidis Fitch[J]. Acta Ecologica Sinica, 2013, 33(15): 4707-4714.[赵曼, 郭线茹, 李为争, 等. 不同玉米品种(系)对玉米蚜生长发育和种群增长的影响[J]. 生态学报, 2013, 33(15): 4707-4714]
Zhou DR, Wang YY, Liu BL,et al. The research of mass rearing of corn borer, Ⅰ. The semi-artificial diet and its improvement[J]. Acta Phytophylacica Sinica, 1980, 7(2): 113-122. [周大荣, 王玉英, 刘宝兰, 等. 玉米螟人工大量繁殖研究:Ⅰ.一种半人工饲料及其改进[J]. 植物保护学报, 1980, 7(2): 113-122]
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