竹节参总皂苷对衰老大鼠结肠炎症反应的改善作用
摘要
目的探讨竹节参总皂苷对衰老大鼠结肠炎症反应的改善作用。方法大鼠随机分为青年组(6月龄)、自然衰老组(24月龄)及竹节参总皂苷低、高剂量组(10、30 mg/kg),每组12只,大鼠从18月龄开始分别给予含10、30 mg/kg竹节参总皂苷的饲料至24月龄。然后,阿利新蓝、高碘酸-Schiff试剂(PAS)染色计算大鼠结肠杯状细胞数量,RT-PCR法检测大鼠结肠组织IL-6、IL-23 mRNA表达,免疫组化法检测大鼠结肠组织STAT3、TLR4、MYD88、TRAF6蛋白表达。结果与自然衰老组比较,竹节参总皂苷组杯状细胞数量显著增加(P<0.05),IL-6、IL-23 mRNA表达及STAT3蛋白表达显著降低(P<0.05,P<0.01),TLR4、MYD88、TRAF6蛋白表达显著升高(P<0.05,P<0.01)。结论竹节参总皂苷可能通过调节TLR4信号通路介导的免疫衰老来改善衰老大鼠结肠炎症反应。
引文
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[2] Soenen S, Rayner C K, Jones K L, et al. The ageing gastrointestinal tract[J]. Curr Opin Clin Nutr Metab Care, 2016, 19(1):12-18.
[3] Liu Y, Yin H, Zhao M, et al. TLR2 and TLR4 in autoimmune diseases: acomprehensive review[J]. Clin Rev Allergy Immunol, 2014, 47(2):136-147.
[4] Liu X, Li H, Lu A, et al. Reduction of intestinal mucosal immune function in heat-stressed rats and bacterial translocation[J]. Int J Hyperthermia, 2012, 28(8):756-765.
[5] 陈静,郭煜晖,刘敏,等.衰老对大鼠小肠形态学及黏膜免疫相关细胞数量的影响[J].广东医学,2017,38(6):839-841,845.
[6] Dun Y Y, Liu M, Chen J, et al. Regulatory effects of saponins from Panax japonicus on colonic epithelial tight junctions in aging rats[J]. J Ginseng Res, 2018, 42(1):50-56.
[7] Mei Y, Xiao Z, Feng J, et al. The preventive effect of total saponins of Panax japonicus on nonsteroidal anti-inflammatory drug-induced enteropathy[J]. Chin J Cell Mol Immunol, 2016, 32(6):734-738.
[8] 张长城,姜美杰,赵海霞,等.竹节参总皂苷对环磷酰胺致免疫低下小鼠免疫功能的影响[J].中成药,2011,33(7):1134-1138.
[9] Gersemann M, Becker S, Kübler I, et al. Differences in goblet cell differentiation between Crohn’s disease and ulcerative colitis[J]. Differentiation, 2009, 77(1):84-94.
[10] Mitsuyama K, Matsumoto S, Rose-John S, et al. STAT3 activation via interleukin 6 trans-signalling contributes to ileitis in SAMP1/Yit mice[J]. Gut, 2006, 55(9):1263-1269.
[11] Zhu X M, Shi Y Z, Cheng M, et al. Serum IL-6, IL-23 profile and Treg/Th17 peripheral cell populations in pediatric patients with inflammatory bowel disease[J]. Pharmazie, 2017, 72(5):283-287.
[12] Mabbott N A, Kobayashi A, Sehgal A, et al. Aging and the mucosal immune system in the intestine[J]. Biogerontology, 2014, 16(2):133-145.
[13] Ko H J, Yang J Y, Shim D H, et al. Innate immunity mediated by MyD88 signal is not essential for induction of lipopolysaccharide-specific B cell responses but is indispensable for protection against Salmonella enterica serovar Typhimurium infection[J]. J Immunol, 2009, 182(4):2305-2312.
[14] Park B S, Lee J O. Recognition of lipopolysaccharide pattern by TLR4 complexes[J]. Exp Mol Med,2013,45:e66.
[15] Sharma R, Kapila R, Haq M R, et al. Age-associated aberrations in mouse cellular and humoral immune responses[J]. Aging Clin Exp Res, 2014, 26(4):353-362.
[16] Jiang X P, Huang X L, Yang Z P, et al. Iguratimod ameliorates inflammatory responses by modulating the Th17/Treg paradigm in dextran sulphate sodium-induced murine colitis[J]. Mol Immunol, 2018, 93:9-19.