Immediate prostaglandin E₂ synthesis in rat 3Y1 fibroblasts following vasopressin V1a receptor stimulation

详细信息	查看全文 | 推荐本文 |

• 作者：Yoshihito Nakatani ; Yuka Chin ; Shuntaro Hara ; Ichiro Kudo
• 关键词：Vasopressin ; Prostaglandin E₂ ; Cytosolic phospholipase A₂α ; Mitogen-activated protein kinase ; Phosphatidylinositol 3-kinase ; V1a receptor
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2007
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：16 March 2007
• 卷：354
• 期：3
• 页码：676-680
• 文件大小：165 K

摘要

Arginine vasopressin (AVP) induces immediate prostaglandin E₂ (PGE₂) production in rat 3Y1 fibroblasts. Judging from effects of several inhibitors, cytosolic phospholipase A₂α (cPLA₂α) and cyclooxygenase-1 (COX-1) were mainly involved in this reaction. The antagonist of vasopressin receptor V1a, and not that of V2, inhibited the AVP-induced PGE₂ synthesis, indicating that AVP activates cPLA₂α through V1a receptor. Treatment of 3Y1 cells with AVP resulted in transient activation of p44/42 mitogen-activated protein kinase (MAPK) and cPLA₂α, and phosphatidylinositol 3-kinase (PI3K) inhibitor blocked not only AVP-induced PGE₂ synthesis but also MAPK activation, suggesting that PI3K is involved in the AVP-induced MAPK and cPLA₂α activation, which initiates the production of PGE₂. These results suggest that PGE₂ generated by the stimulation of AVP probably modulates the physiological effects of AVP.