Isolation and identification of C-19 fatty acids with anti-tumor activity from the spores of Ganoderma lucidum (reishi mushroom)

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• 作者：Pei Gao^a ; Tomoya Hirano^a ; Zhiqing Chen^b ; Tadashi Yasuhara^c ; Yoshihiro Nakata^d ; Akiko Sugimoto^a ; ^{akiko-s.chem@tmd.ac.jp}
• 关键词：Spores of Ganoderma lucidum ; Nonadecanoic acid ; cis-9-Nonadecenoic acid ; Anti-tumor activity ; Identification
• 刊名：Fitoterapia
• 出版年：2012
• 期刊代码：35_0367326x
• 类别：ch
• 出版时间：April, 2012
• 卷：83
• 期：3
• 页码：490-499
• 文件大小：808 K

摘要

We previously showed that ethanolic extracts of spores of Ganoderma lucidum inhibit tumor cell proliferation and induce apoptosis of HL-60 cells. The active constituents appeared to be long-chain fatty acids, particularly carbon-19 (C-19) fatty acids which have not been reported in spores of Ganoderma lucidum. In the present study, two of these C-19 fatty acids which are key compounds in the activities, were identified as their 2-naphthyl ester derivatives after esterification of a mixture of fatty acids obtained from the spores. The active compounds were determines as nonadecanoic acid and cis-9-nonadecenoic acid. The location of the double bond of cis-9-nonadecenoic acid was demonstrated by GC-MS analysis, based on the fragmentation pattern of the adduct prepared from the fatty acid and dimethyl disulfide.