Production of the monoclonal antibody against Sudan 4 for multi-immunoassay of Sudan dyes in egg

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• 作者：Wen Chong Shan^a ; ¹ ; Jian Zhong Xi^a ; ¹ ; Jie Sun^b ; Yu Jie Zhang^a ; Jian Ping Wang^a ; ^{wjpcau@yahoo.com.cn}
• 关键词：Sudan dyes ; Sudan 4 ; Monoclonal antibody ; Heterologous immunoassay ; Egg
• 刊名：Food Control
• 出版年：2012
• 期刊代码：49_09567135
• 类别：abs
• 出版时间：September, 2012
• 卷：27
• 期：1
• 页码：146-152
• 文件大小：351 K

摘要

Sudan dyes (Sudan 1, 2, 3, 4, Para red and Sudan red G) have been banned as food colorants due to their carcinogenicity. This study first synthesized a hapten of Sudan 4 and produced the monoclonal antibody for immunoassay of these red dyes. The hapten was synthesized by coupling 4-amino-3-methylbenzoic acid to o-toluidine to obtain an intermediate hapten and then the intermediate hapten was coupled to 尾-naphthol to obtain a tentative hapten. The generated monoclonal antibody showed broad specificity to the six Sudan dyes. After evaluation of different coating antigens, a heterologous competitive indirect immunoassay was developed to determine the six red dyes in egg yolk simultaneously. The crossreactivities for the six analytes were in a range of 64%-110%and the limits of detection were in a range of 0.08-0.2聽ng/g depending on the compound. Intra- and inter-assay recoveries from the standards fortified blank samples ranged from 71.5%to 107.4%with coefficients of variation lower than 14.6%.