Antigenic stimulation induces recombination activating gene 1 and terminal deoxynucleotidyl transferase expression in a murine T-cell hybridoma

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• 作者：Yuan Zhang^a ; Min Shi^b ; Qian Wen^a ; Wei Luo^a ; Zhi Yang^a ; Mingqian Zhou^a ; Li Ma^a ; ^{maryhmz@126.com}
• 关键词：RAG ; recombination activating gene ; TCR ; T cell receptor ; TdT ; terminal deoxynucleotidyl transferase ; MHC ; major histocompatibility complex ; APCm ; antigen presenting cells ; V ; variable region ; D ; diversity region ; J ; joining region ; RSS ; recombination sig
• 刊名：Cellular Immunology
• 出版年：2012
• 期刊代码：279_00088749
• 类别：med
• 出版时间：2012
• 卷：274
• 期：1-2
• 页码：19-25
• 文件大小：814 K

摘要

Secondary rearrangements of the T cell receptor (TCR) represent a genetic correction mechanism which changes T cell specificity by re-activating V(D)J recombination in peripheral T cells. Murine T-cell hybridoma A1.1 was employed to investigate whether antigenic stimulation induced re-expression of recombinase genes and altered TCR V尾 expression. Following repeated antigenic stimulation, A1.1 cells were induced to re-express recombination activating gene (RAG)1 and terminal deoxynucleotidyl transferase (TdT) which are generally considered prerequisite to TCR gene rearrangement. Accompanied with the significant changes in TCR mRNA levels over time, it is suggested that secondary rearrangements may be induced in A1.1 cells, which represent a mature T cell clone capable of re-expressing RAG genes and possesses the prerequisite for secondary V(D)J rearrangement.