Feasibility and application of liquid-liquid extraction combined with gas chromatography-mass spectrometry for the analysis of phenolic acids from grape polyphenols degraded by human faecal microbiota
- 作者：C. Muñ ; oz-Gonzá ; lez ; M.V. Moreno-Arribas ; J.J. Rodrí ; guez-Bencomo ; C. Cueva ; P.J. Martí ; n Á ; lvarez ; B. Bartolomé ; M.A. Pozo-Bayó ; n ; m.delpozo@csic.es
- 关键词：LLE&ndash ; GC&ndash ; EI-MS ; Phenolic metabolites ; Gut microbiota ; Grape polyphenols
- 刊名：Food Chemistry
- 出版年：2012
- 期刊代码：48_03088146
- 类别：abs
- 出版时间：15 July, 2012
- 卷：133
- 期：2
- 页码：526-535
- 文件大小：390 K
摘要
In this study the feasibility of a LLE-GC-EI-MS method for the analysis of 43 phenolic acids belonging to different chemical structure families which have been described in the literature as microbial-derived metabolites after consumption of dietary polyphenols was proved. In addition, the method was applied for the characterisation of phenolic metabolites resulting from the incubation, in anaerobic conditions, of a commercial grape seed extract (GSE) and their corresponding flavan-3-ol monomeric (GSE-M) and oligomeric (GSE-O) fractions with human faeces from healthy volunteers (n = 3). The method showed average values of repeatability and reproducibility of 5.0%and 6.3%, respectively, adequate and low detection (1.8-30.8 渭g L鈭?) and quantification limits (6.0-102.8 渭g L鈭?) and good recovery values (95%, as average value). A total of 27 phenolic acids were identified in the faecal solutions after incubation with the grape seed extracts. In general, faecal samples incubated with GSE and GSE-M (monomeric fraction) yield a higher formation of phenolic acids compared to the samples incubated with the oligomer fraction (GSE-O).