Analysis of polyphenols and methylxantines in tea samples by means of nano-liquid chromatography utilizing capillary columns packed with core-shell particles

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• 作者：Chiara Fanali^a ; Anna Rocco^b ; ^{anna.rocco@imc.cnr.it} ; Zeineb Aturki^b ; Luigi Mondello^c ; ^a ; Salvatore Fanali^b
• 关键词：Nano-liquid chromatography ; Tea ; Polyphenols ; Methylxantines ; Mass spectrometry ; Core&ndash ; shell stationary phase
• 刊名：Journal of Chromatography A
• 出版年：2012
• 期刊代码：47_00219673
• 类别：ch
• 出版时间：20 April, 2012
• 卷：1234
• 期：Complete
• 页码：38-44
• 文件大小：773 K

摘要

In this study, a rapid separation of eleven polyphenols and three methylxanthines was obtained by means of nano-liquid chromatography (nano-LC), employing a 100 渭m I.D. capillary column packed with C18 core-shell particles (2.7 渭m) for 10 cm. All compounds were baseline resolved with a step gradient elution in less than 15 min. The developed analytical method was validated and the resulting RSD%for intra-day and inter-day repeatability, related to retention time, retention factor and peak area, were below 5.1 and 5.7%. LOD and LOQ values corresponded to 0.300 and 0.625 渭g/mL, while linearity range assessed gave R² no lower than 0.990. Then, the method was used to determine studied compounds in tea extracts. Further, the nano-LC system was coupled with a mass spectrometer to confirm the components present in real samples. Finally the results were compared with those obtained using a capillary column (100 渭m I.D. 脳 10 cm) packed with C18 sub-2 渭m particles, applying the same nano-LC experimental conditions.