pH-Triggered DNA delivery based on multilayer film of DNA polyplexes and charge-reversible poly(ethylenimine)

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• 作者：Jie-Kan Sun ; Ke-Feng Ren ; ^{Renkf@zju.edu.cn} ; Jian Ji ; ^{Jijian@zju.edu.cn}
• 关键词：Charge-reversible ; pH-Triggered delivery ; DNA ; Polyplexes ; Gene delivery ; Multilayer film ; Layer-by-layer complexation
• 刊名：Thin Solid Films
• 出版年：2012
• 期刊代码：78_00406090
• 类别：ph
• 出版时间：1 June, 2012
• 卷：520
• 期：16
• 页码：5426-5430
• 文件大小：614 K

摘要

Development of materials with stimuli-responsive properties is of interest for biotechnical applications including gene delivery and regenerative medicine. Here, we report a multilayer film through layer-by-layer self-assembly of DNA polyplexes and charge-reversible poly(ethylenimine) (cPEI). Through functionalizing PEI with cyclohexanedicarboxylic acid, cPEI showed negatively charged and therefore was used for electrostatic self-assembly with positively charged DNA polyplexes. Side chains of cPEI can be hydrolyzed in acidic environment while it is stable in neutral condition. Such pH-triggered hydrolysis led to charge reverse of cPEI from negative to positive, which consequently led to a disassembly of multilayer film. Both UV-vis and ellipsometry spectrum measurements suggested that the multilayer film grew with a thickness of 150 nm for twelve bilayers. Under low pH condition, the multilayer film collapsed and DNA polyplexes were released. The multilayer film containing cPEI could be served as a local gene delivery system in specific low pH conditions such as extracellular acidity of solid tumor and lysosomal.