Electrochemical immunoassay for carcinoembryonic antigen based on signal ampli铿乧ation strategy of nanotubular mesoporous PdCu alloy

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• 作者：Yanyan Cai^a ; He Li^a ; ^b ; Yuyang Li^a ; Yanfang Zhao^a ; Hongmin Ma^a ; Baocun Zhu^a ; Caixia Xu^a ; Qin Wei^a ; ^{sdjndxwq@163.com} ; Dan Wu^a ; ^{wudan791108@163.com} ; Bin Du^a ; ^{dubin61@gmail.com}
• 关键词：Label-free immunosensor ; Hollow nanostructure ; Graphene ; Carcinoembryonic antigen ; Thionine
• 刊名：Biosensors and Bioelectronics
• 出版年：2012
• 期刊代码：12_09565663
• 类别：ch
• 出版时间：June-July, 2012
• 卷：36
• 期：1
• 页码：6-11
• 文件大小：586 K

摘要

Interests in using nanoporous metals for biosensing applications have been increasing. Herein, nanotubular mesoporous PdCu (NM-PdCu) alloy is used to fabricate a novel label-free electrochemical immunosensor for cancer biomarker carcinoembryonic antigen (CEA). It operates through physisorption of anti-CEA on NM-PdCu and the mixture of sulfonated graphene sheets (HSO₃-GS) and thionine (TH) functionalized glassy carbon electrode interface as the detection platform. In this study, chitosan (CS)-PdCu is bound very strongly to carcinoembryonic antibody (anti-CEA), because of the good electron conductivity, high surface area, and good biocompatibility. CS-PdCu is immobilized on electrodes by electrostatic interactions between the negatively charged sulfo group of HSO₃-GS and the abundant positively charged amino groups of chitosan. TH acts as the redox probe. Under the optimized conditions, the electrochemical immunosensor exhibits a wide working range from 0.01 to 12 ng/mL with a low detection limit of 4.86 pg/mL. The accuracy, reproducibility, and stability of the immunosensor are acceptable. The assay is evaluated for real serum samples, receiving satisfactory results. The nanoporous metal materials-based immunoassay provides a promising approach in clinical application and thus represents a versatile detection method.