Effects of sodium bicarbonate on pathogenicity of Colletotrichum musae and potential for controlling postharvest diseases of banana

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• 作者：D.M. De Costa ; ^{devikacos@yahoo.com} ; H.M.D.M. Gunawardhana ^{dhammika.gunawardhana@yahoo.com}
• 关键词：Biological control ; Colletotrichum musae ; B. spinosa ; GRAS compounds ; Fruit peel characters
• 刊名：Postharvest Biology and Technology
• 出版年：2012
• 期刊代码：89_09255214
• 类别：abs
• 出版时间：June, 2012
• 卷：68
• 期：Complete
• 页码：54-63
• 文件大小：513 K

摘要

The effects of sodium bicarbonate (NaHCO₃) on pathogenicity of Colletotrichum musae and its potential to control postharvest diseases of bananas were determined. Addition of NaHCO₃ reduced mycelial growth, spore production, spore germination and appressoria production of C. musae, in vitro by increasing pH (from 6.9 to 8.7) of the culture medium (PD broth). The pH of 8.59, created by 100 mM NaHCO₃ completely inhibited spore production. Postharvest dip treatment in 300 mM NaHCO₃ for 10 min reduced the lesion area of anthracnose on artificially inoculated banana fruit. Natural infections of anthracnose, crown rot and blossom end rot were also reduced significantly in fruit that were treated with 300 mM NaHCO₃ for 10 min. Efficiency of integrating NaHCO₃ with a bacterial antagonist, Burkholderia spinosa for controlling postharvest diseases of bananas was also determined. Dipping banana fruit in 300 mM NaHCO₃ solution for 10 min followed by dipping in B. spinosa suspension in nutrient broth (cell concentration 1 脳 10⁸ cfu/mL) effectively controlled anthracnose, crown rot and blossom end rot of bananas (var. Kolikuttu). Dipping bananas in 300 mM NaHCO₃ increased pH, total soluble solids and thickness of the fruit peel which may have an indirect or cumulative effect on the reduction of postharvest disease development in bananas.