Proteome-determined type-specific proteins of Mycobacterium avium subspecies paratuberculosis

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• 作者：Valerie Hughes^a ; ^{Val.Hughes@moredun.ac.uk} ; Alfredo Garcia-Sanchez^a ; ¹ ; Stuart Smith^a ; Kevin Mclean^a ; Alex Lainson^a ; Mintu Nath^b ; Karen Stevenson^a
• 关键词：Type I ; Type II ; Mycobacterium ; Paratuberculosis ; Proteome ; 2-D gel electrophoresis
• 刊名：Veterinary Microbiology
• 出版年：2012
• 期刊代码：106_03781135
• 类别：abs
• 出版时间：6 July, 2012
• 卷：158
• 期：1-2
• 页码：153-162
• 文件大小：704 K

摘要

Mycobacterium avium subspecies paratuberculosis (M. a. paratuberculosis) is a pathogen of ruminants, causing paratuberculosis (characterized by severe emaciation). The disease is endemic in many countries including the UK and places a severe economic burden on the global livestock industry.

Two types of M. a. paratuberculosis can be classified by pulsed-field electrophoresis (I/III and II), which are phenotypically distinct and appear to have different host preferences. Proteomes of Type I and Type II M. a. paratuberculosis were analyzed by 2-D gel electrophoresis to determine if any significant differences existed between the subtypes. Seven different strains of Type I and 18 strains of Type II were analyzed and compared to detect type-specific differences. These 鈥榯ype-specific鈥?differences existed regardless of growth phase and were also exhibited in cells isolated directly from pathogenic lesions. Twenty-three spots predominated on the Type I profile, from which 17 proteins were identified. Twenty-one spots predominated on the Type II profile, from which 16 proteins were identified. None of the proteins identified as differentially represented on the profiles of Type I or Type II corresponded to open reading frames of the defining genomic regions as previously described for the Type I (sheep) and Type II (cattle).

Sequence polymorphisms existing in Type I and II strains were identified in some open reading frames or regulatory regions of genes that correspond to proteins expressed in a type-specific fashion. The consequence of these is discussed in relation to protein expression and their impact on the type phenotype is discussed.