Pancreatic stellate cells enhance stem cell-like phenotypes in pancreatic cancer cells

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• 作者：Shin Hamada^a ; ¹ ; Atsushi Masamune^a ; ¹ ; ^{amasamune@med.tohoku.ac.jp} ; Tetsuya Takikawa^a ; ¹ ; Noriaki Suzuki^a ; Kazuhiro Kikuta^a ; Morihisa Hirota^a ; Hirofumi Hamada^b ; Masayoshi Kobune^c ; Kennichi Satoh^d ; Tooru Shimosegawa^a
• 关键词：CSC ; cancer stem cell ; EMT ; epithelial-to-mesenchymal transition ; PSCs ; pancreatic stellate cells ; SV40 ; simian virus 40 ; hTERT ; human telomerase reverse transcriptase
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：4 May, 2012
• 卷：421
• 期：2
• 页码：349-354
• 文件大小：747 K

摘要

The interaction between pancreatic cancer cells and pancreatic stellate cells (PSCs), a major profibrogenic cell type in the pancreas, is receiving increasing attention. There is accumulating evidence that PSCs promote the progression of pancreatic cancer by increasing cancer cell proliferation and invasion as well as by protecting them from radiation- and gemcitabine-induced apoptosis. Recent studies have identified that a portion of cancer cells, called 鈥渃ancer stem cells鈥? within the entire cancer tissue harbor highly tumorigenic and chemo-resistant phenotypes, which lead to the recurrence after surgery or re-growth of the tumor. The mechanisms that maintain the 鈥渟temness鈥?of these cells remain largely unknown. We hypothesized that PSCs might enhance the cancer stem cell-like phenotypes in pancreatic cancer cells. Indirect co-culture of pancreatic cancer cells with PSCs enhanced the spheroid-forming ability of cancer cells and induced the expression of cancer stem cell-related genes ABCG2, Nestin and LIN28. In addition, co-injection of PSCs enhanced tumorigenicity of pancreatic cancer cells in vivo. These results suggested a novel role of PSCs as a part of the cancer stem cell niche.