DNA ploidy measurement in oral leukoplakia: Different results between flow and image cytometry

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• 作者：E.R.E.A. Brouns^a ; ^{5806399@student.acta.nl} ; E. Bloemena^a ; ^b ; ^{e.bloemena@vumc.nl} ; J.A.M. Belien^b ; ^{jam.belien@vumc.nl} ; M.A.M. Broeckaert^b ; ^{mam.broeckaert@vumc.nl} ; I.H.A. Aartman^c ; ^{i.aartman@acta.nl} ; I. van der Waal^a ; ^{i.vanderwaal@vumc.nl}
• 关键词：DNA ploidy ; Flow cytometry ; Image cytometry ; Oral epithelial dysplasia ; Oral leukoplakia ; Oral squamous cell carcinoma ; Potentially malignant disorder
• 刊名：Oral Oncology
• 出版年：2012
• 期刊代码：213_13688375
• 类别：med
• 出版时间：July, 2012
• 卷：48
• 期：7
• 页码：636-640
• 文件大小：170 K

摘要

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Summary

The estimated prevalence of oral leukoplakia is worldwide approximately 2%, with an annual malignant transformation rate of approximately 1%. The aim of the present study was to evaluate the possible contribution of ploidy measurement to the prediction of the clinical course, in a well defined cohort of patients with oral leukoplakia. Ploidy was measured by both flow cytometry (FCM-DNA) and image cytometry (ICM-DNA) and we focussed on the comparison of the two different techniques to determine ploidy. A total of 41 patients have been included, with a mean age of 59 years (range 36-78 years). With FCM-DNA, three lesions were aneuploid, with ICM-DNA, 19 lesions were aneuploid. DNA ploidy was compared with clinicopathological and patients parameters. There were no statistically significant differences between DNA ploidy and any patient factor with both FCM-DNA and ICM-DNA. Using FCM-DNA, DNA aneuploid lesions showed statistically significant more dysplasia (p = 0.04) than diploid lesions. Furthermore, DNA aneuploid lesions were more frequently encountered at high-risk locations (p = 0.03) as being determined with FCM-DNA. These relations were not found when DNA ploidy was determined with ICM-DNA.