Evaluation of antiulcer activity of chromanone fraction from Calophyllum brasiliesnse Camb

详细信息	查看全文 | 推荐本文 |

• 作者：Larissa Maria Scalon Lemos^a ; Thaí ; s Bezerra Martins^a ; Guilherme Henrique Tanajura^a ; Vanessa Fá ; tima Gazoni^a ; Josiane Bonaldo^b ; Claudia Lé ; ia Strada^b ; Marcos Gabriel da Silva^b ; Evandro Luiz Dall’ ; Oglio^b ; Paulo Teixeira de Sousa Jú ; nior^b ; Domingos Tabajara de Oliveira Martins^a ; ^{taba@terra.com.br}
• 关键词：Calophyllum brasiliense ; Clusiaceae ; Chromanone acids ; Gastric ulcer ; Anti-Helicobacter pylori activity
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 期刊代码：26_03788741
• 类别：pha
• 出版时间：7 May, 2012
• 卷：141
• 期：1
• 页码：432-439
• 文件大小：645 K

摘要

Ethnopharmacological relevance

Calophyllum brasiliense Camb. (Clusiaceae), popularly known as 鈥榞uanandi鈥? is found in the tropical areas and swampy lands. The latex exuding from its bark is used in the treatment of gastric ulcer in folk medicine. Several active compounds have been isolated from its stem bark among them, are the chromanone acids. Therefore, this study aimed to evaluate antiulcer activity and probable mechanism(s) of action of a fraction containing a mixture of chromanone acids (BI), derived by column chromatography fractionation of the hexane extract of the stem bark of Calophyllum brasiliense (HECb), using experimental in vitro and in vivo models.

Materials and methods

Ulcer was induced by oral administration of ethanol (75%, v/v) and indomethacin (50 mg/kg). Malondialdehyde, reduced glutathione and catalase activity was measured in stomach tissue after ethanol induced ulcer. In order to evaluate the effect of BI on nitric oxide, ulcer was induced by ethanol in l-NAME pretreated animals. Anti-Helicobacter pylori activity was verified in disk diffusion and broth microdilution in vitro assays, using cagA+ and vacA+ Helicobacter pylori strains.

Results

BI prevented the gastric ulceration caused by ethanol and indomethacin treatments. Its gastroprotective mechanism in ethanol-induced ulcer was partly due to reduction of MDA and CAT levels in the gastric tissue. BI did not affect the GSH levels and its gastroprotective effect was not reversed by pretreatment with l-NAME. BI showed anti-Helicobacter pylori in the both assays.

Conclusion

The results indicate that BI is partly responsible for the HECb antiulcer and anti-Helicobacter pylori effects.