Tissue specificity in nickel uptake and induction of oxidative stress in kidney and spleen of goldfish Carassius auratus, exposed to waterborne nickel

详细信息	查看全文 | 推荐本文 |

• 作者：Olga I. Kubrak^a ; Viktor V. Husak^a ; Bohdana M. Rovenko^a ; Harald Poigner^b ; Maria A. Mazepa^a ; Michael Kriews^c ; Doris Abele^b ; Volodymyr I. Lushchak^a ; ^{lushchak@pu.if.ua}
• 关键词：Nickel toxicity ; Goldfish ; Lymphopenia ; Oxidative stress ; Antioxidant enzymes
• 刊名：Aquatic Toxicology
• 出版年：2012
• 期刊代码：19_0166445x
• 类别：abs
• 出版时间：15 August, 2012
• 卷：118-119
• 期：Complete
• 页码：88-96
• 文件大小：651 K

摘要

Toxic and carcinogenic effects of nickel compounds are suggested to result from nickel-mediated oxidative damage to macromolecules and/or inhibition of cellular antioxidant defenses. We investigated the effects of waterborne Ni²⁺ (10, 25 and 50 mg/L) on the blood and blood-producing tissues (kidney and spleen) of goldfish to identify relationships between Ni accumulation and oxidative stress. Whereas the main hematological parameters (total hemoglobin and hematocrit) were unaffected, Ni²⁺ exposure had substantial influence on goldfish immune system, causing lymphopenia. Ni accumulation increased renal iron content (by 49-78%) and resulted in elevated lipid peroxide (by 29%) and protein carbonyl content (by 274-278%), accompanied by suppression of the activities of superoxide dismutase (by 50-53%), glutathione peroxidase (15-45%), glutathione reductase (31-37%) and glucose-6-phosphate dehydrogenase (20-44%), indicating development of oxidative stress in kidney. In contrast to kidney, in spleen the activation of glutathione peroxidase (by 34-118%), glutathione-S-transferase (by 41-216%) and glutathione reductase (by 47%), as well as constant levels of low molecular mass thiols and metals together with enhanced activity of glucose-6-phosphate dehydrogenase (by 41-94%) speaks for a powerful antioxidant potential that counteracts Ni-induced ROS production. Further, as Ni accumulation in this organ was negligible, Ni-toxicity in spleen may be minimized by efficient exclusion of this otherwise toxic metal.