Colloidal gold-based immunochromatographic assay for detection of botulinum neurotoxin type B
- 作者：Chiao ; Der-Jiang ; Shyu ; Rong-Hwa ; Hu ; Chieh-Shen ; Chiang ; Hsien-Yuan ; Tang ; Shiao-Shek
- 关键词：Immunochromatography ; Botulinum neurotoxin type B ; Colloidal gold
- 刊名：Journal of Chromatography B ; Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2004
- 期刊代码：124_15700232
- 类别：ch
- 出版时间：September 25, 2004
- 卷：809
- 期：1
- 页码：37-41
- 文件大小：119 K
摘要
A rapid immunochromatographic assay was developed to detect botulinum neurotoxin type B (BoNT/B). The assay was based on the sandwich format using polyclonal antibody (Pab). The thiophilic gel purified anti-BoNT/B Pab was immobilized to a defined detection zone on a porous nitrocellulose membrane and conjugated to colloidal gold particles that served as a detection reagent. The BoNT/B-containing sample was added to the membrane and allowed to react with Pab-coated particles. The mixture was then passed along the porous membrane by capillary action past the Pab in the detection zone, which will bind the particles that had BoNT/B bound to their surface, giving a red colour within this detection zone with an intensity proportional to BoNT/B concentration. In the absence of BoNT/B, no immunogold was bound to the solid-phase antibody. With this method, 50ng/ml of BoNT/B was detected in less than 10min. The assay sensitivity can be increased by silver enhancement to 50pg/ml. The developed BoNT/B assay also showed no cross reaction to type A neurotoxin (BoNT/A) and type E neurotoxin (BoNT/E).