An reagentless glucose biosensor based on direct electrochemistry of glucose oxidase immobilized on poly(methylene blue) doped silica nanocomposites

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• 作者：Xiuli Xiao  ; [Author Vitae] ; Bei Zhou  ; [Author Vitae] ; Lian Zhu  ; [Author Vitae] ; Lili Xu  ; [Author Vitae] ; Liang Tan ; ^{liangtan@hunnu.edu.cn}  ; [Author Vitae] ; Hao Tang  ; [Author Vitae] ; Youyu Zhang  ; [Author Vitae] ; Qingji Xie  ; [Author Vitae] ; Shouzhuo Yao  ; [Author Vitae]
• 关键词：PMB ; poly(methylene blue) ; PMB@SiO2(nano) ; poly(methylene blue) doped silica nanocomposites ; GCE ; glassy carbon electrode ; GOx ; glucose oxidase ; FAD ; flavin adenine dinucleotide ; CPNCs ; conducting polymer nanocomposites ; DPV ; differential pulse voltammetr
• 刊名：Sensors and Actuators B ; Chemical
• 出版年：2012
• 期刊代码：161_09254005
• 类别：et
• 出版时间：April, 2012
• 卷：165
• 期：1
• 页码：126-132
• 文件大小：868 K

摘要

Conducting polymer nanocomposites have attracted a wide attention in material chemistry and analytical chemistry. In this work, poly(methylene blue) doped silica nanocomposites (PMB@SiO_2(nano)) on glassy carbon electrode (GCE) were synthesized based on a two-step electropolymerization. Compared with poly(methylene blue) film, PMB@SiO_2(nano) had more advantages in facilitating electron transfer between glucose oxidase (GOx) and the electrode surface. GOx immobilized on PMB@SiO_2(nano) underwent a direct and reversible electrochemical reaction involving two-electron and two-proton exchange. A reagentless glucose biosensor using the nanocomposite modified electrode was developed. The calibration curve was linear over a glucose concentration range of 0.01-1.11 mM with a correlation coefficient of 0.998 and a detection limit (3蟽) of 3 渭M. The apparent Michalis-Menten constant, K_m, for the enzymatic reaction was 0.50 mM. The developed biosensor presented good accuracy, repeatability, reproducibility and stability as well as an excellent ability to resist interference from ascorbic acid and uric acid species. The investigation indicates that PMB@SiO_2(nano) has a good potential for application in construction of the third-generation enzyme biosensor as a conducting matrix material.