Wnt3a promotes melanin synthesis of mouse hair follicle melanocytes
- 作者：Haiying Guo ; Ke Yang ; Fang Deng ; Jixing Ye ; Yizhan Xing ; Yuhong Li ; Xiaohua Lian ; Tian Yang ; tiany@163.net
- 关键词：HF ; hair follicle ; X-gal ; 5-bromo-4-chloro-3-indolyl-尾-d-galactoside ; IRS ; inner root sheath ; McSC ; melanocyte stem cell ; Ad ; adenovirus ; MITF ; microphthalmia-associated transcription factor ; DCT ; dopachrome tautomerase ; TRP1 ; tyrosinase-related protein
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2012
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：20 April, 2012
- 卷：420
- 期：4
- 页码：799-804
- 文件大小：826 K
摘要
Although the importance of Wnt3a in melanocyte development has been well recognized, the effect of Wnt3a in normal HF melanocytes has not been clearly elucidated yet. Thus, we sought to examine the presence and location of Wnt3a in HF during hair cycle. By using melanocyte-targeted Dct-LacZ transgenic mice, we found that Wnt3a signaling is activated in mouse HF melanocytes during anagen of hair cycle. To further explore the potential functions of Wnt3a in mouse melanocytes, we infected melan-a cells with AdWnt3a to serve as the production source of Wnt3a protein. We demonstrated that Wnt3a promoted melanogenesis through upregulation of MITF and its downstream genes, tyrosinase and TRP1, in melanocytes. In vivo, AdWnt3a rescued the effects of AdsimMITF on HF melanocytes and promoted melanin synthesis. Our results suggest that Wnt3a plays an important role in mouse HF melanocytes homeostasis.