Constituents from the Formosan apple reduce tyrosinase activity in human epidermal melanocytes

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• 作者：Yi-Pei Lin ; Feng-Lin Hsu ; Chien-Shu Chen ; Ji-Wang Chern ; Mei-Hsien Lee
• 关键词：Formosan apple ; Malus doumeri var. formosana ; Rosaceae ; 3-Hydroxyphloretin ; Catechol ; Tyrosinase ; Human epidermal melanocytes ; Quantitative real-time PCR ; Molecular docking
• 刊名：Phytochemistry
• 出版年：2007
• 期刊代码：85_00319422
• 类别：ch
• 出版时间：April 2007
• 卷：68
• 期：8
• 页码：1189-1199
• 文件大小：925 K

摘要

Tyrosinase is a copper-containing monooxygenase that catalyzes melanin synthesis in skin melanocytes. Herein, 13 compounds from the Formosan apple (Malus doumeri var. formosana), an indigenous Taiwanese plant, were isolated and identified. The active constituents were identified as 3-hydroxyphloretin (7) and catechol (9); they exhibited potent hydroxyl radical-scavenging (IC₅₀ values, 0.6 and 1.1 μM) and cellular tyrosinase-reducing activities (IC₅₀ values, 32 and 22 μM) in human epidermal melanocytes. In addition, we evaluated the level of several tyrosinase-related proteins by Western blot analysis. In contrast to 3-hydroxyphloretin (7), which showed no effect on the level of these proteins, catechol (9) reduced their activity and the expression of the respective genes, as determined by quantitative real-time PCR. In a kinetic analysis of mushroom tyrosinase, 3-hydroxyphloretin (7) was a competitive inhibitor. These two constituents exhibited metal-coordinating interactions with copper ions in a virtual model of molecular docking with human tyrosinase. Thus, 3-hydroxyphloretin (7) and catechol (9) were the most active constituents from the Formosan apple; they exhibited anti-oxidant and tyrosinase reducing activities, suggesting their possible use as cosmetic agents.