Expression of a murine homolog of apoptosis-inducing human IL-24/MDA-7 in murine tumors fails to induce apoptosis or produce anti-tumor effects

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• 作者：Hiroyasu Nagakawa^a ; ^b ; Osamu Shimozato^a ; Ling Yu^a ; Akihiko Wada^a ; ^b ; Kiyoko Kawamura^a ; Quanhai Li^a ; ^c ; Sunil Chada^d ; Yuji Tada^b ; Yuichi Takiguchi^e ; Koichiro Tatsumi^b ; Masatoshi Tagawa^a ; ^c ; ^{mtagawa@chiba-cc.jp}
• 关键词：IL ; interleukin ; IFN ; interferon ; TNF ; tumor necrosis factor ; MDA-7 ; differentiation-associated gene 7 ; Ad ; adenoviruses ; Th1 ; T helper 1 ; IL-24R ; interleukin-24 receptors ; RT&ndash ; PCR ; reverse transcription&ndash ; polymerase chain reaction ; MOI ; multipli
• 刊名：Cellular Immunology
• 出版年：2012
• 期刊代码：279_00088749
• 类别：med
• 出版时间：January-February, 2012
• 卷：275
• 期：1-2
• 页码：90-97
• 文件大小：1143 K

摘要

Expression of human interleukin (IL)-24 in tumors achieved anti-tumor effects through apoptosis. IL-24 also induced secretion of proinflammatory cytokines, suggesting the role in immunity. We showed that murine IL-24 transcripts started from the second initiation codon and that expressed mIL-24 in tumors failed to induce apoptosis. Proliferation of murine cells expressing mIL-24 was the same as that of the parent cells and inoculation of the mIL-24-expressing tumors into syngeneic mice did not produce anti-tumor effects. Secretory mIL-24 did not induce the expression of the IL-6, TNF-伪 or IFN-纬 gene in spleen cells. Expression of mIL-24 receptor subunits, IL-22R and IL-20R1, was undetectable in spleen cells even though they were stimulated by anti-CD3, anti-CD40 antibody or concanavalin A. Transduction of murine tumors with adenoviruses expressing the human IL-24 gene however suppressed the viability and decreased the tumor growth. These data suggest that mIL-24 is functionally irrelevant to the human counterpart.