- 作者：Fumitake Usuia ; Hiroaki Kimuraa ; Taichi Ohshirob ; Kazuki Tatsumia ; Akira Kawashimaa ; Akiyo Nishiyamaa ; Yo-ichiro Iwakurac ; Shun Ishibashib ; Masafumi Takahashia ; masafumi2@jichi.ac.jp
- 关键词：Atherosclerosis ; Cytokines ; Diet ; Inflammation ; Macrophages
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2012
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：30 March, 2012
- 卷：420
- 期：1
- 页码：72-77
- 文件大小：1003 K
Objective
Several reports describe the role of interleukin (IL)-17 in the development of atherosclerosis; however, its precise role remains controversial. We generated double-deficient mice for apolipoprotein E (apoE) and IL-17 (apoE鈭?鈭?/sup>IL-17鈭?鈭?/sup> mice) and investigated the effect of IL-17 deficiency on vascular inflammation and atherosclerosis.Methods and results
Atherosclerotic plaque areas in apoE鈭?鈭?/sup>IL-17鈭?鈭?/sup> mice fed a Western diet (WD) were significantly reduced compared with those in apoE鈭?鈭?/sup> mice. No significant differences in plasma lipid profiles were observed between apoE鈭?鈭?/sup> and apoE鈭?鈭?/sup>IL-17鈭?鈭?/sup> mice. The number of infiltrated macrophages in the plaques was significantly decreased in WD-fed apoE鈭?鈭?/sup>IL-17鈭?鈭?/sup> mice compared with WD-fed apoE鈭?鈭?/sup> mice, whereas vascular smooth muscle cell content was not altered by IL-17 deficiency. Expression of inflammatory cytokines (MCP-1, IL-1尾, IL-6, IFN-纬, and IL-12 p40) and scavenger receptors (Msr-1, Scarb1, and Olr1) in the plaques was inhibited in WD-fed apoE鈭?鈭?/sup>IL-17鈭?鈭?/sup> mice. Furthermore, expression of inducible nitric oxide (M1 marker) and arginase-1 (M2 marker) was inhibited in WD-fed apoE鈭?鈭?/sup>IL-17鈭?鈭?/sup> mice. Our results indicate that IL-17 deficiency reduces vascular inflammation and atherosclerosis and that modulation of IL-17 could be a potential target for prevention and treatment of atherosclerosis.Conclusion