Measuring ligand-receptor binding events on polymeric surfaces with periodic wave patterns using liquid crystals
- 作者：Gyeo-Re Han ; Chang-Hyun Jang ; chjang4u@kyungwon.ac.kr
- 关键词：Liquid crystals ; 4-Cyano-4&prime ; -pentylbiphenyl (5CB) ; Polymeric substrates ; Buckling ; Ligand&ndash ; receptor binding ; Sensors
- 刊名：Colloids and Surfaces B ; Biointerfaces
- 出版年：2012
- 期刊代码：14_09277765
- 类别：ms
- 出版时间：1 June, 2012
- 卷：94
- 期：Complete
- 页码：89-94
- 文件大小：1100 K
摘要
In this study, we measured ligand-receptor binding events on polymeric substrates with periodic nanostructures using liquid crystals (LCs). Periodic sinusoidal wave patterns were generated through the buckling of the poly-(dimethylsiloxane) (PDMS) substrate on a cylindrical surface followed by replicating the associated relief structures on a poly-(urethane acrylate) (PUA) surface, where a film of gold was deposited. Avidin was then covalently immobilized onto a gold surface decorated with carboxylic acid-terminated self-assembled monolayer via NHS/EDC chemistry. The optical response of the device showed that the orientation of nematic 4-cyano-4鈥?pentylbiphenyl (5CB) was parallel to the plane of the avidin surface. However, the formation of the avidin-biotin complexes disturbed the sinusoidal topographies of the surface, and induced a random orientation of LCs, which produced a distinctive change in the optical response. We also confirmed the specific ligand-receptor interactions using ellipsometry and atomic force microscopy (AFM). These results suggest that polymeric surfaces with continuous wavy features could be used to develop novel LC-based sensors for the detection of ligand-receptor binding events.