Dysregulation of overexpressed IL-32伪 in hepatocellular carcinoma suppresses cell growth and induces apoptosis through inactivation of NF-魏B and Bcl-2

详细信息	查看全文 | 推荐本文 |

• 作者：Yun Hee Kang^a ; Mi-Young Park^a ; Do-Young Yoon^b ; Seung Ro Han^c ; Chung Il Lee^a ; Na Young Ji^a ; Pyung-Keun Myung^d ; Hee Gu Lee^a ; Jae Wha Kim^a ; Young Il Yeom^a ; Ye Jin Jang^a ; Dong Kuk Ahn^c ; Jong Wan Kim^e ; Eun Young Song^a ; ^{eysong@kribb.re.kr}
• 关键词：IL-32伪 ; Hepatocellular carcinoma ; Cell growth ; Apoptosis ; Diagnostic marker
• 刊名：Cancer Letters
• 出版年：2012
• 期刊代码：44_03043835
• 类别：med
• 出版时间：28 May, 2012
• 卷：318
• 期：2
• 页码：226-233
• 文件大小：1443 K

摘要

IL-32 is a newly discovered cytokine. Recently, various reports suggest that it plays a role as a proinflammatory mediator and may be involved in several cancer carcinogenesis. However, IL-32 expression in hepatocellular carcinoma (HCC) remains unclear. In this study, we investigated the expression and role of IL-32伪 in hepatocellular carcinoma, because IL-32 was identified as an upregulated gene in hepatocellular carcinoma tissues compared to nontumorous regions using DNA microarray. IL-32伪 was overexpressed in tissue and serum from patients with HCC and localized in the cytoplasm and nucleus of hepatocellular carcinoma tumor cells. Moreover, secreted IL-32伪 concentration in the serum of patients with hepatocellular carcinoma was elevated as compared with those in the normal serum using a developed sandwich ELISA. Furthermore, IL-32伪 suppression in hepatocellular carcinoma decreased expression of phospho-p38 MAPK, NF-魏B, and antiapoptotic protein Bcl-2 and induced expression of proapoptotic proteins as well as p53 and PUMA resulting in the suppression of cell growth and induction of intrinsic apoptosis. Based on our results, we suggest that IL-32伪 is involved in the progression of hepatocellular carcinoma and may be a useful biomarker for diagnosis and therapeutic target of hepatocellular carcinoma.