Metabolism of fatty acid in yeast: Characterisation of β-oxidation and ultrastructural changes in the genus Sporidiobolus sp. cultivated on ricinoleic acid methyl ester

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• 作者：Gilles Feron ; Caroline Blin-Perrin ; Isabelle Krasniewski ; Geneviè ; ve Mauvais and Jeannine Lherminier
• 关键词：Yeast β ; -oxidation ; Sporidiobolus genus ; Mitochondrion ; Ricinoleic acid ; γ ; -Decalactone
• 刊名：FEMS Microbiology Letters
• 出版年：2005
• 期刊代码：34_03781097
• 类别：imm
• 出版时间：2005
• 卷：250
• 期：1
• 页码：63-69
• 文件大小：336 K

摘要

Cell structure modifications and β-oxidation induction were monitored in two strains of Sporidiobolus, Sp. Ruinenii and Sp. pararoseus after cultivation on ricinoleic acid methyl ester. Ultrastructural observations of the yeast before and after cultivation on fatty acid esters did not reveal major modifications in Sp. ruinenii. Unexpectedly, in Sp. pararoseus a proliferation of the mitochondrion was observed. After induction, Sp. ruinenii principally exhibited an increase in the activities of acyl-CoA oxidase (ACO), hydroxyacyl-CoA deshydrogenase (HAD), thiolase and catalase. In contrast, Sp. pararoseus lacked ACO and catalase activities, but an increase in acyl-CoA deshydrogenase (ACDH) and enoyl-CoA hydratase (ECH) activity was observed. These data suggest that in Sp. ruinenii, β-oxidation is preferentially localized in the microbody, whereas in Sp. pararoseus it might be localized in the mitochondria.