1伪,25-Dihydroxyvitamin D3 inhibits osteoblastic differentiation of mouse periodontal fibroblasts

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• 作者：Yen-Chen Chen^a ; ^b ; Tadashi Ninomiya^c ; Akihiro Hosoya^d ; Toru Hiraga^d ; Hiroo Miyazawa^c ; Hiroaki Nakamura^d ; ^{nakam@po.mdu.ac.jp}
• 关键词：Vitamin D3 ; Periodontal fibroblasts ; Osteoblastic differentiation ; PLAP-1
• 刊名：Archives of Oral Biology
• 出版年：2012
• 期刊代码：25_00039969
• 类别：med
• 出版时间：May, 2012
• 卷：57
• 期：5
• 页码：453-459
• 文件大小：1041 K

摘要

Objective

Periodontal ligament (PDL) is a non-mineralized tissue connecting cementum and alveolar bone. Recent studies have suggested that periodontal fibroblasts can differentiate into mineralized tissue-forming cells such as osteoblasts and cementoblasts. We elucidated the immunolocalization of vitamin D receptor (VDR) and the effects of vitamin D₃ (VD₃) on mouse periodontal fibroblasts to clarify the role of VDR and VD₃ in the differentiation of periodontal fibroblasts.

Design

Immunohistochemical analysis using anti-VDR antibody was performed in paraffin sections of mouse mandibles at E13, E14, E17, P10, and P35. The roles of VD₃ in osteoblastic differentiation and mineralization were estimated by alkaline phosphatase (ALP) and alizarin red (AR) staining. In addition, the mRNA expression of osteoblast markers and mineralization inhibitors was examined by real-time PCR.

Results

At the bud, cap and early bell stages, immunoreactivity for VDR was scarcely seen in the cells of dental follicles. Labelling was detected in the nuclei of cemetoblasts, periodontal fibroblasts and osteoblasts during root formation. VD₃ inhibited ALP activity and AR-positive mineralized nodule formation of periodontal fibroblasts in osteogenic culture medium. Real-time PCR revealed that VD₃ down-regulated the levels of osteoblast markers. In contrast, VD₃ significantly up-regulated the level of periodontal ligament associated protein (PLAP)-1, a negative regulator of osteoblastic differentiation.

Conclusion

These results suggest that VD₃ negatively regulates the osteoblastic differentiation of mouse periodontal fibroblasts and prevents the periodontal ligament from mineralization via increase of PLAP-1.