Porcine reproductive and respiratory syndrome virus induces CD4⁺CD8⁺CD25⁺Foxp3⁺ regulatory T cells (Tregs)

详细信息	查看全文 | 推荐本文 |

• 作者：Erika Silva-Campa^a ; Veró ; nica Mata-Haro^a ; Enric Mateu^b ; Jesú ; s Herná ; ndez^a ; ^{jhdez@ciad.mx}
• 关键词：PRRSV ; Regulatory T cells ; Foxp3 ; IL-10 ; TGF-尾
• 刊名：Virology
• 出版年：2012
• 期刊代码：108_00426822
• 类别：imm
• 出版时间：15 August, 2012
• 卷：430
• 期：1
• 页码：73-80
• 文件大小：941 K

摘要

The aim of this study was to analyze the regulatory T cells (Tregs) induced by the porcine reproductive and respiratory syndrome virus (PRRSV) in pigs. Serum, blood, tonsil, and mediastinal lymph nodes' samples were obtained at different time post-infection (dpi). The frequencies of CD4⁺CD8^{鈭?/sup>CD25+Foxp3+, CD4+CD8+CD25+Foxp3+, or CD4鈭?/sup>CD8+CD25+Foxp3+ phenotypes were determined in PBMC and lymph node cells, and cells producing IL-10 or TGF-尾 were analyzed. PRRSV increased the number of CD4+CD8+CD25+Foxp3+ cells at 14 dpi, whereas CD4+CD8鈭?/sup>CD25+Foxp3+ remained constant until 28 dpi. Positive correlation exists between viremia and induced regulatory cells. CD4+CD8+CD25+Foxp3+-induced Treg cells were consistently observed in lymphoid tissues. Analysis of IL-10- and TGF-尾-producing cell demonstrated that in response to PRRSV, CD4+CD8鈭?/sup>Foxp3low and CD4+CD8+Foxp3high cells increase moderately the proportion of IL-10+ cells. TGF-尾 was only observed in the CD4+CD8+Foxp3high population after PRRSV stimulation. In conclusion, PRRSV infection increases the frequency of Tregs with the phenotype CD4+CD8+CD25+Foxp3high and produces TGF-尾.}