The next step? Use of tissue fusion technology to perform the serial transverse enteroplasty鈥攑roof of principle

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• 作者：Megha Suri^a ; ^b ; ^c ; Bryan Dicken^d ; Patrick N. Nation^b ; Pamela Wizzard^b ; Justine M. Turner^b ; ^e ; Paul W. Wales^a ; ^b ; ^c ; ^{paul.wales@sickkids.ca}
• 关键词：Serial transverse enteroplasty ; Tissue fusion technology ; Radiofrequency energy ; Short bowel syndrome ; intestinal failure
• 刊名：Journal of Pediatric Surgery
• 出版年：2012
• 期刊代码：190_00223468
• 类别：med
• 出版时间：May, 2012
• 卷：47
• 期：5
• 页码：938-943
• 文件大小：443 K

摘要

Background

Serial transverse enteroplasty (STEP) is an established procedure for intestinal lengthening and tapering. A gastrointestinal linear stapler is used to taper the bowel sequentially. We report preliminary experience with tissue fusion technology to perform STEP in a porcine model.

Methods

Four weaned male pigs (mean age, 4 卤 0 weeks; mean weight, 6.8 卤 0.1 kg) first underwent a 60-cm reversed intestinal segment followed by a STEP 4 to 6 weeks later. The LigaSure Impact (Covidien, Valleylab, Tyco Healthcare Group LP, Boulder, CO) was used to perform the procedure. Animals were fed on postoperative day 2 and terminated 1 week later. Morphometric data were collected, and intestinal tissue was obtained for histology.

Results

Mean bowel caliber of 5.1 卤 0.5 cm was tapered to 1.8 卤 0.3 cm post-STEP with a mean increase in the length of the dilated segment of 82%卤 20%. All animals tolerated enteral feeding, and all survived to termination on day 7. Histologic evaluation revealed the zone of tissue fusion to be 7.1 卤 1.5 mm. Masson trichrome, hematoxylin and eosin, and polarized picrosirius red stains demonstrated that the fusion zone was well healed with overlying granulation tissue.

Conclusion

This is the first report of the successful application of radiofrequency energy to perform the STEP procedure in animals. Although further evaluation is required, tissue fusion devices may eventually provide an alternative to the linear stapler for the STEP procedure.