ECM spreading behaviour on micropatterned TiO₂ nanotube surfaces

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• 作者：Andreas Pittrof^a ; ¹ ; Jung Park^b ; ^c ; ¹ ; Sebastian Bauer^a ; Patrik Schmuki^a ; ^{schmuki@ww.uni-erlangen.de}
• 关键词：TiO2 nanotubes ; Mesenchymal stem cells ; ECM ; Micropatterning ; Photolithography
• 刊名：Acta Biomaterialia
• 出版年：2012
• 期刊代码：1_17427061
• 类别：ce
• 出版时间：July, 2012
• 卷：8
• 期：7
• 页码：2639-2647
• 文件大小：2307 K

摘要

By electrochemical anodization, highly ordered nanotubular TiO₂ structures were formed on titanium surfaces with diameters of 15 and 100 nm. In previous work we showed that 15 nm tubes strongly enhanced adhesion and vitality of many cell types, whereas on 100 nm diameter tubes the induction of apoptosis was observed. In the present work we produce mixed (15 nm contrasted with 100 nm) nanotube microstructures that combine highly defined micro- and nanostructures using a photolithographic approach to achieve a direct comparison of adhesion and spreading of mesenchymal stem cells on different diameter nanotubes present on a single surface. On these coupled different nanoscale surfaces mesenchymal stem cell adhesion is initially favoured on 15 nm tube areas but, with time, a gradient in cell number and shape to the 鈥渦nfavourable鈥?regions of the substrate (100 nm tubes) can be observed. This can be explained by cells on the 鈥渇avourable鈥?15 nm regions that strongly produce and shed extracellular matrix onto the 鈥渦nfavourable鈥?locations. These findings contribute to the design of cell guiding surfaces, but also demonstrate the need for a long-range defined homogeneous order when studying cell behaviour on nanostructured surfaces.