A novel role of brain-type ACS4 isotype in neuronal differentiation

详细信息	查看全文 | 推荐本文 |

• 作者：Yong-Yeon Cho ; ^{yongyeon@catholic.ac.kr}
• 关键词：ACS4 ; Brain-type ACS4 isotype ; Neuronal differentiation ; In vitro differentiation ; ACS4 knockout embryonic stem cell
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：16 March, 2012
• 卷：419
• 期：3
• 页码：505-510
• 文件大小：1320 K

摘要

Acyl-CoA synthetase (ACS4) is an arachidonate preferring acyl-CoA synthetase and has been shown to play an important role in lipid metabolism. Although arachidonate and decosahexanoate play a key role in lipid metabolism and in the brain, the mechanisms of its action are still not clearly understood. In this study, we identified brain-type ACS4 mRNA by 5鈥?RACE. We also confirmed that the mRNA in TT2 ES cells encoded a novel ACS4 protein, which contains 41 amino acids at its N-terminus. Furthermore, we found that ACS4 proteins were up-regulated in PC-12 cell by stimulation with nerve growth factor (NGF). Importantly, knockout of ACS4 in ES cells markedly attenuated neuronal differentiation induced by NGF and all-trans retinoic acids (RA). These results indicate that the ACS4 protein specifically expressed in brain plays an important role in arachidonate metabolism and neuronal differentiation in the brain.