Chaeoglobosin Fex inhibits poly(I:C)-induced activation of bone marrow-derived dendritic cells

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• 作者：Lin Sun^a ; Chunyan Hua^a ; Yonghong Yang^a ; Huan Dou^a ; Erguang Li^a ; Renxiang Tan^b ; ^{yayihou@nju.edu.cn} ; Yayi Hou^a ; ^{rxtan@nju.edu.cn}
• 关键词：DCs ; dendritic cells ; Cha Fex ; Chaeoglobosin Fex ; BMDCs ; bone marrow-derived dendritic cells ; APCs ; antigen presenting cells ; IL-6 ; interleukin-6 ; IL-12 ; interleukin-12 ; TNF-伪 ; tumor necrosis factor-伪 ; MCP-1 ; monocyte chemoattractant protein-1 ; Cha K ; C
• 刊名：Molecular Immunology
• 出版年：2012
• 期刊代码：112_01615890
• 类别：bio
• 出版时间：June, 2012
• 卷：51
• 期：2
• 页码：150-158
• 文件大小：1222 K

摘要

Dendritic cells (DCs) are implicated in the induction of autoimmune diseases and exist in lesions associated with several autoimmune inflammatory diseases. Chaeoglobosin Fex (Cha Fex), a cytochalasan-based alkaloid, was isolated from marine-derived endophytic fungus Chaetomium globosum QEN-14. In the present study, we evaluated the effect of Cha Fex on poly(I:C)-induced bone marrow-derived DCs. The results showed that Cha Fex attenuated the production of IFN-尾 both at the mRNA and protein level in poly(I:C)-induced DCs. Cha Fex markedly inhibited the maturation and function of the DCs with a reduced capacity to uptake antigens and low level of expression of costimulatory molecules. Moreover, Cha Fex abrogated the ability of poly(I:C)-induced DCs to promotion of T cell proliferation, Furthermore, Cha Fex inhibited the phosphorylation of I魏B-伪 and IRF-3 in poly(I:C)-induced DCs. Cha Fex also reduced the phosphorylation of p38 and JNK, without affecting ERK1/2. These data demonstrate that that Cha Fex can exhibit an immunosuppressive effect on mouse bone marrow-derived DCs (BMDCs) via TLR3 signaling, which suggests potential application of Cha Fex in the treatment of autoimmune inflammatory diseases.