Bovine herpesvirus type 1 (BHV-1) mutant lacking U_L49.5 luminal domain residues 30-32 and cytoplasmic tail residues 80-96 induces more rapid onset of virus neutralizing antibody and cellular immune responses in calves than the wild-type strain

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• 作者：Huiyong Wei ; Junyun He ; Daniel B. Paulsen ; Shafiqul I. Chowdhury ; ^{chowdh@lsu.edu}
• 关键词：BHV-1 ; Bovine herpesvirus type 1 ; TAP ; transporter associated with antigen processing ; MHC-I ; major histocompatibility complex class I molecules ; CT ; cytoplasmic tail ; MDBK cells ; Madin-Darby bovine kidney cells ; VN Ab ; virus neutralization antibody
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：2012
• 期刊代码：105_01652427
• 类别：abs
• 出版时间：30 June, 2012
• 卷：147
• 期：3-4
• 页码：223-229
• 文件大小：508 K

摘要

Bovine herpesvirus type 1 (BHV-1) envelope protein U_L49.5 inhibits transporter associated with antigen processing (TAP) and down-regulates cell-surface expression of major histocompatibility complex (MHC) class I molecules to promote immune evasion. Earlier, we have constructed a BHV-1U_L49.5螖30-32 CT-null virus and determined that in the infected cells, TAP inhibition and MHC-I down regulation properties of the virus are abolished. In this study, we compared the pathogenicity and immune responses in calves infected with BHV-1U_L49.5螖30-32 CT-null and BHV-1 wt viruses. Following primary infection, both BHV-1 wt and BHV-1U_L49.5螖30-32 CT-null virus replicated in the nasal epithelium with very similar yields. BHV-1 antigen-specific CD8⁺ T cell proliferation as well as CD8⁺ T cell cytotoxicity in calves infected with the BHV-1U_L49.5螖30-32 CT-null virus peaked by 7 dpi (P < 0.05) which is 7 days earlier than that of BHV-1 wt-infected calves. Further, virus neutralizing antibody (VN Ab) titers and IFN-纬 producing peripheral blood mononuclear cells (PBMCs) in the U_L49.5 mutant virus-infected calves, also peaked 7 days (IFN-纬; P < 0.05) and 14 days (VN Ab; P < 0.05) earlier, respectively. Therefore, relative to wt in the BHV-1U_L49.5 mutant virus-infected calves, primary neutralizing antibody and cellular immune responses were induced significantly more rapidly.